Restriction map for pF1KB7730
 (Linear) (Six-base) MAPSORT of: KIBB7730.seq  Check: 9429  from: 1  to: 1440



KIBB7730.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AatII G_ACGT'C

Cuts at:      0     140    1440 
   Size:        140    1300
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1266    1320    1440 
   Size:       1266      54     120

  Fragments arranged by size: 

                1266     120      54
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     700    1440 
   Size:        700     740
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     391     430    1440 
   Size:        117     274      39    1010

  Fragments arranged by size: 

                1010     274     117      39
 
AvaI C'yCGr_G

Cuts at:      0      13      78     276     608    1440 
   Size:         13      65     198     332     832

  Fragments arranged by size: 

                 832     332     198      65      13
 
BamHI G'GATC_C

Cuts at:      0    1175    1440 
   Size:       1175     265
 
BanI G'GyrC_C

Cuts at:      0       9     709     928    1036    1079    1311    1440 
   Size:          9     700     219     108      43     232     129

  Fragments arranged by size: 

                 700     232     219     129     108      43       9
 
BanII G_rGCy'C

Cuts at:      0     358     463    1440 
   Size:        358     105     977

  Fragments arranged by size: 

                 977     358     105
 
BbeI G_GCGC'C

Cuts at:      0      13    1083    1440 
   Size:         13    1070     357

  Fragments arranged by size: 

                1070     357      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     132    1017    1440 
   Size:        132     885     423

  Fragments arranged by size: 

                 885     423     132
 
BbvCI CC'TCA_GC

Cuts at:      0     384    1440 
   Size:        384    1056
 
BglI GCCn_nnn'nGGC

Cuts at:      0     325     404    1440 
   Size:        325      79    1036

  Fragments arranged by size: 

                1036     325      79
 
Bme1580I G_kGCm'C

Cuts at:      0     712    1440 
   Size:        712     728
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     427    1440 
   Size:        427    1013
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     413     422     560     810    1371    1440 
   Size:        107     306       9     138     250     561      69

  Fragments arranged by size: 

                 561     306     250     138     107      69       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     384     839    1440 
   Size:        384     455     601

  Fragments arranged by size: 

                 601     455     384
 
BsaHI Gr'CG_yC

Cuts at:      0      10     137     370     629    1080    1369    1440 
   Size:         10     127     233     259     451     289      71

  Fragments arranged by size: 

                 451     289     259     233     127      71      10
 
BsaWI w'CCGG_w

Cuts at:      0     238    1190    1345    1440 
   Size:        238     952     155      95

  Fragments arranged by size: 

                 952     238     155      95
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     484     514    1369    1399    1440 
   Size:        484      30     855      30      41

  Fragments arranged by size: 

                 855     484      41      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     626    1258    1374    1440 
   Size:        626     632     116      66

  Fragments arranged by size: 

                 632     626     116      66
 
BseYI C'CCAG_C

Cuts at:      0     430     713    1440 
   Size:        430     283     727

  Fragments arranged by size: 

                 727     430     283
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     623    1120    1307    1440 
   Size:        361     262     497     187     133

  Fragments arranged by size: 

                 497     361     262     187     133
 
BsiEI CG_ry'CG

Cuts at:      0      44    1022    1440 
   Size:         44     978     418

  Fragments arranged by size: 

                 978     418      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     463    1440 
   Size:        463     977
 
BsmI GAATG_Cn'

Cuts at:      0      57    1440 
   Size:         57    1383
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     213     578    1440 
   Size:        213     365     862

  Fragments arranged by size: 

                 862     365     213
 
Bsp1286I G_dGCh'C

Cuts at:      0     358     463     712    1440 
   Size:        358     105     249     728

  Fragments arranged by size: 

                 728     358     249     105
 
BspEI T'CCGG_A

Cuts at:      0    1345    1440 
   Size:       1345      95
 
BsrBI CCG'CTC

Cuts at:      0     190     443    1440 
   Size:        190     253     997

  Fragments arranged by size: 

                 997     253     190
 
BsrDI GCAATG_nn'

Cuts at:      0     954    1440 
   Size:        954     486
 
BsrFI r'CCGG_y

Cuts at:      0     759     819     931    1033    1440 
   Size:        759      60     112     102     407

  Fragments arranged by size: 

                 759     407     112     102      60
 
BssHII G'CGCG_C

Cuts at:      0     202    1440 
   Size:        202    1238
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     117    1440 
   Size:        117    1323
 
BstEII G'GTnAC_C

Cuts at:      0    1425    1440 
   Size:       1425      15
 
BstYI r'GATC_y

Cuts at:      0    1175    1440 
   Size:       1175     265
 
BtgI C'CryG_G

Cuts at:      0     560     590    1433    1440 
   Size:        560      30     843       7

  Fragments arranged by size: 

                 843     560      30       7
 
BtsI GCAGTG_nn'

Cuts at:      0     373    1440 
   Size:        373    1067
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     248     283     936     971    1098    1133    1440 
   Size:        248      35     653      35     127      35     307

  Fragments arranged by size: 

                 653     307     248     127      35      35      35
 
EaeI y'GGCC_r

Cuts at:      0      41     933    1019    1401    1440 
   Size:         41     892      86     382      39

  Fragments arranged by size: 

                 892     382      86      41      39
 
EagI C'GGCC_G

Cuts at:      0      41    1019    1440 
   Size:         41     978     421

  Fragments arranged by size: 

                 978     421      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     796    1023    1210    1440 
   Size:        796     227     187     230

  Fragments arranged by size: 

                 796     230     227     187
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1246    1440 
   Size:       1246     194
 
EcoICRI GAG'CTC

Cuts at:      0     461    1440 
   Size:        461     979
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     413     422     560     810    1266    1320    1371 
   Size:        107     306       9     138     250     456      54      51

Cuts at:   1371    1440 
   Size:         69

  Fragments arranged by size: 

                 456     306     250     138     107      69      54      51
                   9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     397    1098    1440 
   Size:        397     701     342

  Fragments arranged by size: 

                 701     397     342
 
FspI TGC'GCA

Cuts at:      0      56    1440 
   Size:         56    1384
 
HaeII r_GCGC'y

Cuts at:      0      13    1083    1440 
   Size:         13    1070     357

  Fragments arranged by size: 

                1070     357      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     980    1012    1167    1199    1234    1258    1266    1290 
   Size:        980      32     155      32      35      24       8      24

Cuts at:   1290    1440 
   Size:        150

  Fragments arranged by size: 

                 980     155     150      35      32      32      24      24
                   8
 
HincII GTy'rAC

Cuts at:      0     888    1146    1440 
   Size:        888     258     294

  Fragments arranged by size: 

                 888     294     258
 
HpaI GTT'AAC

Cuts at:      0    1146    1440 
   Size:       1146     294
 
KasI G'GCGC_C

Cuts at:      0       9    1079    1440 
   Size:          9    1070     361

  Fragments arranged by size: 

                1070     361       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1226    1390    1440 
   Size:       1226     164      50

  Fragments arranged by size: 

                1226     164      50
 
MslI CAynn'nnrTG

Cuts at:      0     664    1440 
   Size:        664     776
 
MspA1I CmG'CkG

Cuts at:      0     114     322     388     455     592     785    1291    1440 
   Size:        114     208      66      67     137     193     506     149

  Fragments arranged by size: 

                 506     208     193     149     137     114      67      66
            
 
NaeI GCC'GGC

Cuts at:      0     761    1035    1440 
   Size:        761     274     405

  Fragments arranged by size: 

                 761     405     274
 
NarI GG'CG_CC

Cuts at:      0      10    1080    1440 
   Size:         10    1070     360

  Fragments arranged by size: 

                1070     360      10
 
NcoI C'CATG_G

Cuts at:      0    1433    1440 
   Size:       1433       7
 
NgoMIV G'CCGG_C

Cuts at:      0     759    1033    1440 
   Size:        759     274     407

  Fragments arranged by size: 

                 759     407     274
 
NspI r_CATG'y

Cuts at:      0    1263    1440 
   Size:       1263     177
 
PasI CC'CwG_GG

Cuts at:      0     424    1440 
   Size:        424    1016
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     430    1076    1440 
   Size:        430     646     364

  Fragments arranged by size: 

                 646     430     364
 
PpuMI rG'GwC_Cy

Cuts at:      0    1098    1440 
   Size:       1098     342
 
PvuII CAG'CTG

Cuts at:      0     114     388    1291    1440 
   Size:        114     274     903     149

  Fragments arranged by size: 

                 903     274     149     114
 
SacI G_AGCT'C

Cuts at:      0     463    1440 
   Size:        463     977
 
SacII CC_GC'GG

Cuts at:      0     593    1440 
   Size:        593     847
 
SanDI GG'GwC_CC

Cuts at:      0    1098    1440 
   Size:       1098     342
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1023    1210    1440 
   Size:       1023     187     230

  Fragments arranged by size: 

                1023     230     187
 
SexAI A'CCwGG_T

Cuts at:      0     638    1440 
   Size:        638     802
 
SfcI C'TryA_G

Cuts at:      0     213     482     777    1440 
   Size:        213     269     295     663

  Fragments arranged by size: 

                 663     295     269     213
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     404    1440 
   Size:        404    1036
 
SfoI GGC'GCC

Cuts at:      0      11    1081    1440 
   Size:         11    1070     359

  Fragments arranged by size: 

                1070     359      11
 
SmaI CCC'GGG

Cuts at:      0      80     278    1440 
   Size:         80     198    1162

  Fragments arranged by size: 

                1162     198      80
 
SphI G_CATG'C

Cuts at:      0    1263    1440 
   Size:       1263     177
 
StuI AGG'CCT

Cuts at:      0     695    1440 
   Size:        695     745
 
StyI C'CwwG_G

Cuts at:      0    1433    1440 
   Size:       1433       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     537     546     727    1440 
   Size:        537       9     181     713

  Fragments arranged by size: 

                 713     537     181       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     484     516    1369    1401    1440 
   Size:        484      32     853      32      39

  Fragments arranged by size: 

                 853     484      39      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     628    1102    1440 
   Size:        628     474     338

  Fragments arranged by size: 

                 628     474     338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     973    1440 
   Size:        973     467
 
XmaI C'CCGG_G

Cuts at:      0      78     276    1440 
   Size:         78     198    1164

  Fragments arranged by size: 

                1164     198      78
 
ZraI GAC'GTC

Cuts at:      0     138    1440 
   Size:        138    1302
 

 Enzymes that do cut:

    AatII     AcuI     AlfI    AlwNI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI Bme1580I     BplI     BpmI   Bpu10I
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI   BstEII    BstYI     BtgI     BtsI    CspCI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
   HincII     HpaI     KasI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI    PflMI    PpuMI    PvuII     SacI
    SacII    SanDI     SapI    SexAI     SfcI     SfiI     SfoI     SmaI
     SphI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI
    BsiWI    BspHI    BspMI    BsrGI    BssSI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     KpnI     MfeI     MluI
     MscI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI     SbfI     ScaI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI
     XbaI     XhoI     XmnI
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