Restriction map for pF1KB7719
 (Linear) (Six-base) MAPSORT of: KIBB7719.seq  Check: 9503  from: 1  to: 1281



KIBB7719.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     184    1139    1281 
   Size:        184     955     142

  Fragments arranged by size: 

                 955     184     142
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     343     563     808     979    1281 
   Size:        126     217     220     245     171     302

  Fragments arranged by size: 

                 302     245     220     217     171     126
 
AflIII A'CryG_T

Cuts at:      0     848    1281 
   Size:        848     433
 
AhdI GACnn_n'nnGTC

Cuts at:      0     849    1074    1281 
   Size:        849     225     207

  Fragments arranged by size: 

                 849     225     207
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      43    1147    1281 
   Size:         43    1104     134

  Fragments arranged by size: 

                1104     134      43
 
AvaI C'yCGr_G

Cuts at:      0    1119    1281 
   Size:       1119     162
 
BanII G_rGCy'C

Cuts at:      0     935    1281 
   Size:        935     346
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     206     240    1281 
   Size:        206      34    1041

  Fragments arranged by size: 

                1041     206      34
 
BglII A'GATC_T

Cuts at:      0    1274    1281 
   Size:       1274       7
 
Bme1580I G_kGCm'C

Cuts at:      0      62    1281 
   Size:         62    1219
 
BmgBI CAC'GTC

Cuts at:      0     897    1281 
   Size:        897     384
 
BmtI G_CTAG'C

Cuts at:      0    1162    1281 
   Size:       1162     119
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1206    1281 
   Size:       1206      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     449     498     940    1281 
   Size:         59     390      49     442     341

  Fragments arranged by size: 

                 442     390     341      59      49
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77     749    1281 
   Size:         77     672     532

  Fragments arranged by size: 

                 672     532      77
 
BsaHI Gr'CG_yC

Cuts at:      0     219     410    1043    1281 
   Size:        219     191     633     238

  Fragments arranged by size: 

                 633     238     219     191
 
BsaWI w'CCGG_w

Cuts at:      0      50    1281 
   Size:         50    1231
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     532     562    1281 
   Size:        532      30     719

  Fragments arranged by size: 

                 719     532      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     349     352     391     534     537     540     994     997 
   Size:        349       3      39     143       3       3     454       3

Cuts at:    997    1198    1281 
   Size:        201      83

  Fragments arranged by size: 

                 454     349     201     143      83      39       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     526     665    1281 
   Size:        526     139     616

  Fragments arranged by size: 

                 616     526     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319    1057    1248    1281 
   Size:        319     738     191      33

  Fragments arranged by size: 

                 738     319     191      33
 
BsiHKAI G_wGCw'C

Cuts at:      0    1190    1256    1281 
   Size:       1190      66      25

  Fragments arranged by size: 

                1190      66      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1020    1281 
   Size:       1020     261
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     935    1190    1256    1281 
   Size:         62     873     255      66      25

  Fragments arranged by size: 

                 873     255      66      62      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184    1139    1175    1281 
   Size:        184     955      36     106

  Fragments arranged by size: 

                 955     184     106      36
 
BsrDI GCAATG_nn'

Cuts at:      0      14     392    1275    1281 
   Size:         14     378     883       6

  Fragments arranged by size: 

                 883     378      14       6
 
BsrFI r'CCGG_y

Cuts at:      0     236    1281 
   Size:        236    1045
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     993    1281 
   Size:        993     288
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     405    1281 
   Size:        405     876
 
BstYI r'GATC_y

Cuts at:      0      65     615    1274    1281 
   Size:         65     550     659       7

  Fragments arranged by size: 

                 659     550      65       7
 
BtgI C'CryG_G

Cuts at:      0     839    1281 
   Size:        839     442
 
BtsI GCAGTG_nn'

Cuts at:      0     222    1084    1281 
   Size:        222     862     197

  Fragments arranged by size: 

                 862     222     197
 
EaeI y'GGCC_r

Cuts at:      0      14    1001    1281 
   Size:         14     987     280

  Fragments arranged by size: 

                 987     280      14
 
EarI CTCTTCn'nnn_

Cuts at:      0     582    1281 
   Size:        582     699
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     126     343     449     498     563     808     940 
   Size:         59      67     217     106      49      65     245     132

Cuts at:    940     979    1281 
   Size:         39     302

  Fragments arranged by size: 

                 302     245     217     132     106      67      65      59
                  49      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1180    1281 
   Size:       1180     101
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     987    1281 
   Size:        987     294
 
FspI TGC'GCA

Cuts at:      0    1171    1281 
   Size:       1171     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564     748     780     835     867    1060    1092 
   Size:        532      32     184      32      55      32     193      32

Cuts at:   1092    1281 
   Size:        189

  Fragments arranged by size: 

                 532     193     189     184      55      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1103    1281 
   Size:        467     636     178

  Fragments arranged by size: 

                 636     467     178
 
MscI TGG'CCA

Cuts at:      0    1003    1281 
   Size:       1003     278
 
MslI CAynn'nnrTG

Cuts at:      0     107     262    1281 
   Size:        107     155    1019

  Fragments arranged by size: 

                1019     155     107
 
MspA1I CmG'CkG

Cuts at:      0    1103    1205    1281 
   Size:       1103     102      76

  Fragments arranged by size: 

                1103     102      76
 
NaeI GCC'GGC

Cuts at:      0     238    1281 
   Size:        238    1043
 
NcoI C'CATG_G

Cuts at:      0     839    1281 
   Size:        839     442
 
NgoMIV G'CCGG_C

Cuts at:      0     236    1281 
   Size:        236    1045
 
NheI G'CTAG_C

Cuts at:      0    1158    1281 
   Size:       1158     123
 
NspI r_CATG'y

Cuts at:      0     852     991    1281 
   Size:        852     139     290

  Fragments arranged by size: 

                 852     290     139
 
PciI A'CATG_T

Cuts at:      0     848    1281 
   Size:        848     433
 
PfoI T'CCnGG_A

Cuts at:      0     853    1064    1281 
   Size:        853     211     217

  Fragments arranged by size: 

                 853     217     211
 
PstI C_TGCA'G

Cuts at:      0    1091    1281 
   Size:       1091     190
 
SexAI A'CCwGG_T

Cuts at:      0     695    1281 
   Size:        695     586
 
SfcI C'TryA_G

Cuts at:      0    1087    1281 
   Size:       1087     194
 
SmaI CCC'GGG

Cuts at:      0    1121    1281 
   Size:       1121     160
 
StuI AGG'CCT

Cuts at:      0     232     343    1281 
   Size:        232     111     938

  Fragments arranged by size: 

                 938     232     111
 
StyI C'CwwG_G

Cuts at:      0     206     839    1281 
   Size:        206     633     442

  Fragments arranged by size: 

                 633     442     206
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     101    1281 
   Size:        101    1180
 
Tth111I GACn'n_nGTC

Cuts at:      0     697    1281 
   Size:        697     584
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     721    1281 
   Size:        721     560
 
XmaI C'CCGG_G

Cuts at:      0    1119    1281 
   Size:       1119     162
 
XmnI GAAnn'nnTTC

Cuts at:      0     274    1281 
   Size:        274    1007
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AlfI     AvaI    BanII     BcgI
    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI     BtsI
     EaeI     EarI  Eco57MI    EcoNI     FalI     FspI    Hin4I     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI
     PciI     PfoI     PstI    SexAI     SfcI     SmaI     StuI     StyI
    TaqII  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com