Restriction map for pF1KB7573
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        347  GGTACC               G^GTACC
AccI          547  GTCTAC               GT^[AC][GT]AC
AlwI          597  GATCC                GGATC(4/5)
ApaI          394  GGGCCC               GGGCC^C
Asp718I       347  GGTACC               G^GTACC
AspHI          64  GAGCTC               G[AT]GC[AT]^C
AvaII         570  GGTCC                G^G[AT]CC
BpmI          459  CTGGAG               CTGGAG(16/14)
BsaI          331  GAGACC               GGTCTC(1/5)
BsiEI         143  CGGCCG               CG[GA][CT]^CG
BsiHKAI        64  GAGCTC               G[AT]GC[AT]^C
BsmFI          18  GTCCC                GGGAC(10/14)
BspMI         612  GCAGGT               ACCTGC(4/8)
BsrFI         618  GCCGGT               [GA]^CCGG[CT]
BstAPI        609  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         596  AGATCC               [GA]^GATC[CT]
Cfr10I        618  GCCGGT               [GA]^CCGG[CT]
DpnII         597  GATC                 ^GATC
DraII         393  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI            9  GACTTGTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          566  CCATGG               C^C[GA][CT]GG
EagI          143  CGGCCG               C^GGCCG
EarI          586  CTCTTC               CTCTTC(1/4)
EclXI         143  CGGCCG               C^GGCCG
Eco52I        143  CGGCCG               C^GGCCG
EcoNI         366  CCTGGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      393  GGGGCCC              [GA]G^G[ACGT]CC[CT]
FauI          517  CCCGC                CCCGC(4/6)
KpnI          347  GGTACC               GGTAC^C
Ksp632I       586  CTCTTC               CTCTTC(1/4)
MboI          597  GATC                 ^GATC
MboII         587  TCTTC                GAAGA(8/7)
MflI          596  AGATCC               [GA]^GATC[CT]
MslI          411  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          609  GCAGCAGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          566  CCATGG               C^CATGG
NdeII         597  GATC                 ^GATC
PaeR7I        472  CTCGAG               C^TCGAG
PflMI         573  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          462  GAGTC                GAGTC(4/5)
SacI           64  GAGCTC               GAGCT^C
Sau3AI        597  GATC                 ^GATC
ScaI          353  AGTACT               AGT^ACT
SexAI         343  ACCAGGT              A^CC[AT]GGT
SfcI          481  CTACAG               C^T[GA][CT]AG
TaqI          473  TCGA                 T^CGA
TfiI          630  GATTC                G^A[AT]TC
Tsp509I        80  AATT                 ^AATT
TthHB8I       473  TCGA                 T^CGA
Van91I        573  CCAGTACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          472  CTCGAG               C^TCGAG
XhoII         596  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BfaI          449  CTAG                 C^TAG
              493  CTAG                
BsmAI         130  GAGAC                GTCTC(1/5)
              331  GAGAC               
Cac8I         319  GCCAGC               GC[ACGT]^[ACGT]GC
              602  GCAAGC              
DdeI          204  CTCAG                C^T[ACGT]AG
              314  CTAAG               
EaeI           32  TGGCCG               [CT]^GGCC[GA]
              143  CGGCCG              
HinfI         462  GAGTC                G^A[ACGT]TC
              630  GATTC               
HphI          285  GGTGA                GGTGA(8/7)
              369  GGTGA               
MaeI          449  CTAG                 C^TAG
              493  CTAG                
SmaI          335  CCCGGG               CCC^GGG
              404  CCCGGG              
SmlI          292  CTCAAG               C^T[CT][GA]AG
              472  CTCGAG              
TspRI         399  CAGTG                CA[GC]TG[ACGT][ACGT]^
              521  CAGTG               
XmaCI         335  CCCGGG               C^CCGGG
              404  CCCGGG              
XmaI          335  CCCGGG               C^CCGGG
              404  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        BanI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BsrI        
BssKI       BstNI       Cfr13I      EcoRII      EcoT14I     
Fnu4HI      HaeIII      HapII       HpaII       ItaI        
MaeIII      MnlI        MspI        MvaI        NciI        
NlaIII      NlaIV       RsaI        Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp45I      
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