Restriction map for pF1KB7573
 (Linear) (Six-base) MAPSORT of: KIBB7573.seq  Check: 640  from: 1  to: 636



KIBB7573.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     603     636 
   Size:        603      33
 
AccI GT'mk_AC

Cuts at:      0     548     636 
   Size:        548      88
 
Acc65I G'GTAC_C

Cuts at:      0     347     636 
   Size:        347     289
 
ApaI G_GGCC'C

Cuts at:      0     398     636 
   Size:        398     238
 
AvaI C'yCGr_G

Cuts at:      0     335     404     472     636 
   Size:        335      69      68     164

  Fragments arranged by size: 

                 335     164      69      68
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     554     587     636 
   Size:        554      33      49

  Fragments arranged by size: 

                 554      49      33
 
BanI G'GyrC_C

Cuts at:      0     340     347     615     636 
   Size:        340       7     268      21

  Fragments arranged by size: 

                 340     268      21       7
 
BanII G_rGCy'C

Cuts at:      0      68     398     516     636 
   Size:         68     330     118     120

  Fragments arranged by size: 

                 330     120     118      68
 
Bme1580I G_kGCm'C

Cuts at:      0     343     382     398     405     636 
   Size:        343      39      16       7     231

  Fragments arranged by size: 

                 343     231      39      16       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     392     636 
   Size:        392     244
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     636 
   Size:        480     156
 
Bpu10I CC'TnA_GC

Cuts at:      0     314     636 
   Size:        314     322
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     636 
   Size:        277     359
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325     636 
   Size:        325     311
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     412     442     636 
   Size:        412      30     194

  Fragments arranged by size: 

                 412     194      30
 
BseYI C'CCAG_C

Cuts at:      0     555     625     636 
   Size:        555      70      11

  Fragments arranged by size: 

                 555      70      11
 
BsiEI CG_ry'CG

Cuts at:      0     146     636 
   Size:        146     490
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     636 
   Size:         68     568
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     343     382     398     405     516     636 
   Size:         68     275      39      16       7     111     120

  Fragments arranged by size: 

                 275     120     111      68      39      16       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     603     636 
   Size:        603      33
 
BsrFI r'CCGG_y

Cuts at:      0     618     636 
   Size:        618      18
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     615     636 
   Size:        615      21
 
BstYI r'GATC_y

Cuts at:      0     596     636 
   Size:        596      40
 
BtgI C'CryG_G

Cuts at:      0     566     636 
   Size:        566      70
 
BtsI GCAGTG_nn'

Cuts at:      0     527     636 
   Size:        527     109
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      15     636 
   Size:         15     621
 
EaeI y'GGCC_r

Cuts at:      0      32     143     636 
   Size:         32     111     493

  Fragments arranged by size: 

                 493     111      32
 
EagI C'GGCC_G

Cuts at:      0     143     636 
   Size:        143     493
 
EarI CTCTTCn'nnn_

Cuts at:      0     592     636 
   Size:        592      44
 
EcoICRI GAG'CTC

Cuts at:      0      66     636 
   Size:         66     570
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     636 
   Size:        480     156
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     370     636 
   Size:        370     266
 
EcoO109I rG'GnC_Cy

Cuts at:      0     394     636 
   Size:        394     242
 
KpnI G_GTAC'C

Cuts at:      0     351     636 
   Size:        351     285
 
MslI CAynn'nnrTG

Cuts at:      0     415     636 
   Size:        415     221
 
NcoI C'CATG_G

Cuts at:      0     566     636 
   Size:        566      70
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     579     636 
   Size:        579      57
 
PspOMI G'GGCC_C

Cuts at:      0     394     636 
   Size:        394     242
 
PspXI vC'TCGA_Gb

Cuts at:      0     472     636 
   Size:        472     164
 
SacI G_AGCT'C

Cuts at:      0      68     636 
   Size:         68     568
 
ScaI AGT'ACT

Cuts at:      0     355     636 
   Size:        355     281
 
SexAI A'CCwGG_T

Cuts at:      0     343     636 
   Size:        343     293
 
SfcI C'TryA_G

Cuts at:      0     481     636 
   Size:        481     155
 
SmaI CCC'GGG

Cuts at:      0     337     406     636 
   Size:        337      69     230

  Fragments arranged by size: 

                 337     230      69
 
SmlI C'TyrA_G

Cuts at:      0     292     472     636 
   Size:        292     180     164

  Fragments arranged by size: 

                 292     180     164
 
StyI C'CwwG_G

Cuts at:      0     156     165     566     636 
   Size:        156       9     401      70

  Fragments arranged by size: 

                 401     156      70       9
 
TatI w'GTAC_w

Cuts at:      0     353     575     636 
   Size:        353     222      61

  Fragments arranged by size: 

                 353     222      61
 
XhoI C'TCGA_G

Cuts at:      0     472     636 
   Size:        472     164
 
XmaI C'CCGG_G

Cuts at:      0     335     404     636 
   Size:        335      69     232

  Fragments arranged by size: 

                 335     232      69
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     ApaI     AvaI     BaeI     BanI    BanII
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI    BstYI     BtgI
     BtsI     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     KpnI     MslI     NcoI    PflMI   PspOMI    PspXI     SacI
     ScaI    SexAI     SfcI     SmaI     SmlI     StyI     TatI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XmnI     ZraI
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