Restriction map for pF1KB7571
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfcI        SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tru9I       Tsp509I     Van91I      
XbaI        XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          319  GGCGCC               G[GA]^CG[CT]C
AfaI           21  GTAC                 GT^AC
AlwI           47  GGATC                GGATC(4/5)
ApaI          570  GGGCCC               GGGCC^C
AspHI         127  GAGCTC               G[AT]GC[AT]^C
AspI          168  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         252  GGACC                G^G[AT]CC
BbeI          319  GGCGCC               GGCGC^C
BbvCI         575  CCTCAGC              CCTCAGC(-5/-2)
BfaI          136  CTAG                 C^TAG
BpmI          226  CTGGAG               CTGGAG(16/14)
BsaHI         319  GGCGCC               G[GA]^CG[CT]C
BseRI         457  GAGGAG               GAGGAG(10/8)
BsiEI         351  CGGCCG               CG[GA][CT]^CG
BsiHKAI       127  GAGCTC               G[AT]GC[AT]^C
BsmAI         460  GAGAC                GTCTC(1/5)
BsrFI          39  GCCGGC               [GA]^CCGG[CT]
BsrI          171  CCAGT                ACTGG(1/-1)
BstEII        165  GGTGACC              G^GT[ACGT]ACC
BstPI         165  GGTGACC              G^GT[ACGT]ACC
BstXI         300  CCACATGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         39  GCCGGC               [GA]^CCGG[CT]
EaeI          351  CGGCCG               [CT]^GGCC[GA]
EagI          351  CGGCCG               C^GGCCG
EclXI         351  CGGCCG               C^GGCCG
Eco52I        351  CGGCCG               C^GGCCG
EcoO65I       165  GGTGACC              G^GT[ACGT]ACC
Hin1I         319  GGCGCC               G[GA]^CG[CT]C
HphI          165  GGTGA                GGTGA(8/7)
KasI          319  GGCGCC               G^GCGCC
MaeI          136  CTAG                 C^TAG
MaeIII        166  GTGAC                ^GT[ACGT]AC
MslI          301  CACATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        345  CAGCGG               C[AC]G^C[GT]G
NaeI           39  GCCGGC               GCC^GGC
NarI          319  GGCGCC               GG^CGCC
NgoMIV         39  GCCGGC               G^CCGGC
NspI          275  ACATGC               [GA]CATG^[CT]
PaeR7I        124  CTCGAG               C^TCGAG
PflFI         168  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         251  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI           21  GTAC                 GT^AC
SacI          127  GAGCTC               GAGCT^C
SapI          129  GCTCTTC              GCTCTTC(1/4)
SexAI         254  ACCTGGT              A^CC[AT]GGT
SfoI          319  GGCGCC               GGC^GCC
SgrAI          38  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI          124  CTCGAG               C^T[CT][GA]AG
StuI          155  AGGCCT               AGG^CCT
TfiI          427  GAATC                G^A[AT]TC
Tsp45I        166  GTGAC                ^GT[GC]AC
TspRI         214  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       168  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          124  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         440  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
              578  CAGCACCTG           
BanI           99  GGTGCC               G^G[CT][GA]CC
              319  GGCGCC              
BbsI          186  GAAGAC               GAAGAC(2/6)
              292  GAAGAC              
BbvI          231  GCAGC                GCAGC(8/12)
              344  GCAGC               
BpuAI         186  GAAGAC               GAAGAC(2/6)
              292  GAAGAC              
DdeI          431  CTGAG                C^T[ACGT]AG
              576  CTCAG               
DpnII          48  GATC                 ^GATC
               57  GATC                
DraII         251  AGGACCT              [GA]G^G[ACGT]CC[CT]
              570  GGGCCCC             
EcoO109I      251  AGGACCT              [GA]G^G[ACGT]CC[CT]
              570  GGGCCCC             
FauI           36  CCCGC                CCCGC(4/6)
              323  CCCGC               
MboI           48  GATC                 ^GATC
               57  GATC                
NdeII          48  GATC                 ^GATC
               57  GATC                
Sau3AI         48  GATC                 ^GATC
               57  GATC                
SfaNI         218  GCATC                GCATC(5/9)
              262  GCATC               
SmaI          116  CCCGGG               CCC^GGG
              567  CCCGGG              
TaqI          125  TCGA                 T^CGA
              221  TCGA                
TseI          231  GCAGC                G^C[AT]GC
              344  GCAGC               
TthHB8I       125  TCGA                 T^CGA
              221  TCGA                
XmaCI         116  CCCGGG               C^CCGGG
              567  CCCGGG              
XmaI          116  CCCGGG               C^CCGGG
              567  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BanII       
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
EarI        EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       PleI        Sau96I      ScrFI       
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