Restriction map for pF1KB7562
 (Linear) (Six-base) MAPSORT of: KIBB7562.seq  Check: 6995  from: 1  to: 822



KIBB7562.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     822 
   Size:         85     737
 
AleI CACnn'nnGTG

Cuts at:      0       8     822 
   Size:          8     814
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      40     740     822 
   Size:         40     700      82

  Fragments arranged by size: 

                 700      82      40
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     485     517     822 
   Size:        485      32     305

  Fragments arranged by size: 

                 485     305      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     195     822 
   Size:        195     627
 
ApaI G_GGCC'C

Cuts at:      0     325     822 
   Size:        325     497
 
AvaI C'yCGr_G

Cuts at:      0     378     625     822 
   Size:        378     247     197

  Fragments arranged by size: 

                 378     247     197
 
BamHI G'GATC_C

Cuts at:      0     762     822 
   Size:        762      60
 
BanII G_rGCy'C

Cuts at:      0     287     317     325     822 
   Size:        287      30       8     497

  Fragments arranged by size: 

                 497     287      30       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     554     822 
   Size:        554     268
 
BbvCI CC'TCA_GC

Cuts at:      0     308     822 
   Size:        308     514
 
BglI GCCn_nnn'nGGC

Cuts at:      0     208     822 
   Size:        208     614
 
BlpI GC'TnA_GC

Cuts at:      0     273     398     822 
   Size:        273     125     424

  Fragments arranged by size: 

                 424     273     125
 
Bme1580I G_kGCm'C

Cuts at:      0     253     325     454     582     822 
   Size:        253      72     129     128     240

  Fragments arranged by size: 

                 253     240     129     128      72
 
BmgBI CAC'GTC

Cuts at:      0     700     822 
   Size:        700     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     382     395     430     568     652     822 
   Size:        263     119      13      35     138      84     170

  Fragments arranged by size: 

                 263     170     138     119      84      35      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     308     822 
   Size:        308     514
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794     822 
   Size:        794      28
 
BsaAI yAC'GTr

Cuts at:      0     234     822 
   Size:        234     588
 
BsaHI Gr'CG_yC

Cuts at:      0     128     822 
   Size:        128     694
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205     597     627     822 
   Size:        175      30     392      30     195

  Fragments arranged by size: 

                 392     195     175      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433     792     822 
   Size:        433     359      30

  Fragments arranged by size: 

                 433     359      30
 
BseYI C'CCAG_C

Cuts at:      0      92     296     393     436     514     589     654     766 
   Size:         92     204      97      43      78      75      65     112

Cuts at:    766     822 
   Size:         56

  Fragments arranged by size: 

                 204     112      97      92      78      75      65      56
                  43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     214     822 
   Size:        118      96     608

  Fragments arranged by size: 

                 608     118      96
 
BsiHKAI G_wGCw'C

Cuts at:      0     719     822 
   Size:        719     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     287     317     325     454     582     719     822 
   Size:        253      34      30       8     129     128     137     103

  Fragments arranged by size: 

                 253     137     129     128     103      34      30       8
            
 
BspHI T'CATG_A

Cuts at:      0     426     822 
   Size:        426     396
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     732     822 
   Size:        732      90
 
BsrBI CCG'CTC

Cuts at:      0     405     822 
   Size:        405     417
 
BsrDI GCAATG_nn'

Cuts at:      0      24     822 
   Size:         24     798
 
BsrFI r'CCGG_y

Cuts at:      0     101     822 
   Size:        101     721
 
BssSI C'ACGA_G

Cuts at:      0     145     822 
   Size:        145     677
 
BstYI r'GATC_y

Cuts at:      0     185     762     822 
   Size:        185     577      60

  Fragments arranged by size: 

                 577     185      60
 
BtgI C'CryG_G

Cuts at:      0     254     822 
   Size:        254     568
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     263     382     395     430     568     652     822 
   Size:         85     178     119      13      35     138      84     170

  Fragments arranged by size: 

                 178     170     138     119      85      84      35      13
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178     822 
   Size:        178     644
 
EcoO109I rG'GnC_Cy

Cuts at:      0     322     659     822 
   Size:        322     337     163

  Fragments arranged by size: 

                 337     322     163
 
HaeII r_GCGC'y

Cuts at:      0     246     822 
   Size:        246     576
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207     250     282     822 
   Size:        175      32      43      32     540

  Fragments arranged by size: 

                 540     175      43      32      32
 
MslI CAynn'nnrTG

Cuts at:      0       8     236     822 
   Size:          8     228     586

  Fragments arranged by size: 

                 586     228       8
 
MspA1I CmG'CkG

Cuts at:      0      63     360     481     507     822 
   Size:         63     297     121      26     315

  Fragments arranged by size: 

                 315     297     121      63      26
 
NaeI GCC'GGC

Cuts at:      0     103     822 
   Size:        103     719
 
NcoI C'CATG_G

Cuts at:      0     254     822 
   Size:        254     568
 
NgoMIV G'CCGG_C

Cuts at:      0     101     822 
   Size:        101     721
 
PasI CC'CwG_GG

Cuts at:      0     687     822 
   Size:        687     135
 
PmlI CAC'GTG

Cuts at:      0     234     822 
   Size:        234     588
 
PpuMI rG'GwC_Cy

Cuts at:      0     659     822 
   Size:        659     163
 
PspOMI G'GGCC_C

Cuts at:      0     321     822 
   Size:        321     501
 
PspXI vC'TCGA_Gb

Cuts at:      0     625     822 
   Size:        625     197
 
PstI C_TGCA'G

Cuts at:      0     200     557     707     822 
   Size:        200     357     150     115

  Fragments arranged by size: 

                 357     200     150     115
 
PvuII CAG'CTG

Cuts at:      0      63     360     507     822 
   Size:         63     297     147     315

  Fragments arranged by size: 

                 315     297     147      63
 
SbfI CC_TGCA'GG

Cuts at:      0     200     557     822 
   Size:        200     357     265

  Fragments arranged by size: 

                 357     265     200
 
SfcI C'TryA_G

Cuts at:      0     196     553     703     822 
   Size:        196     357     150     119

  Fragments arranged by size: 

                 357     196     150     119
 
SmaI CCC'GGG

Cuts at:      0     380     822 
   Size:        380     442
 
SmlI C'TyrA_G

Cuts at:      0     625     809     822 
   Size:        625     184      13

  Fragments arranged by size: 

                 625     184      13
 
StyI C'CwwG_G

Cuts at:      0      44     254     471     822 
   Size:         44     210     217     351

  Fragments arranged by size: 

                 351     217     210      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     529     561     822 
   Size:        529      32     261

  Fragments arranged by size: 

                 529     261      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     651     822 
   Size:        651     171
 
XhoI C'TCGA_G

Cuts at:      0     625     822 
   Size:        625     197
 
XmaI C'CCGG_G

Cuts at:      0     378     822 
   Size:        378     444
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     AloI    AlwNI     ApaI     AvaI    BamHI
    BanII     BbsI    BbvCI     BglI     BlpI Bme1580I    BmgBI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
    BstYI     BtgI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MslI
   MspA1I     NaeI     NcoI   NgoMIV     PasI     PmlI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SbfI     SfcI     SmaI     SmlI     StyI
     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BmrI     BmtI     BplI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XmnI     ZraI
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