Restriction map for pF1KB7552
 (Linear) (Six-base) MAPSORT of: KIBB7552.seq  Check: 2132  from: 1  to: 804



KIBB7552.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     804 
   Size:        541     263
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     470     804 
   Size:        470     334
 
AlwNI CAG_nnn'CTG

Cuts at:      0     590     804 
   Size:        590     214
 
BanI G'GyrC_C

Cuts at:      0     514     804 
   Size:        514     290
 
BanII G_rGCy'C

Cuts at:      0     690     804 
   Size:        690     114
 
BbeI G_GCGC'C

Cuts at:      0     518     804 
   Size:        518     286
 
BbvCI CC'TCA_GC

Cuts at:      0     142     804 
   Size:        142     662
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     226     804 
   Size:        226     578
 
BfrBI ATG'CAT

Cuts at:      0     528     804 
   Size:        528     276
 
BglII A'GATC_T

Cuts at:      0     563     804 
   Size:        563     241
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     453     804 
   Size:        453     351
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     126     281     804 
   Size:        126     155     523

  Fragments arranged by size: 

                 523     155     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735     804 
   Size:        735      69
 
Bpu10I CC'TnA_GC

Cuts at:      0     142     147     804 
   Size:        142       5     657

  Fragments arranged by size: 

                 657     142       5
 
BsaHI Gr'CG_yC

Cuts at:      0     515     804 
   Size:        515     289
 
BsaWI w'CCGG_w

Cuts at:      0     229     804 
   Size:        229     575
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     171     201     268     298     804 
   Size:        171      30      67      30     506

  Fragments arranged by size: 

                 506     171      67      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     212     228     257     273     804 
   Size:        212      16      29      16     531

  Fragments arranged by size: 

                 531     212      29      16      16
 
BseYI C'CCAG_C

Cuts at:      0     200     804 
   Size:        200     604
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     751     804 
   Size:        430     321      53

  Fragments arranged by size: 

                 430     321      53
 
Bsp1286I G_dGCh'C

Cuts at:      0     690     804 
   Size:        690     114
 
BspEI T'CCGG_A

Cuts at:      0     229     804 
   Size:        229     575
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     395     566     734     804 
   Size:        395     171     168      70

  Fragments arranged by size: 

                 395     171     168      70
 
BstYI r'GATC_y

Cuts at:      0     137     563     804 
   Size:        137     426     241

  Fragments arranged by size: 

                 426     241     137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     467     804 
   Size:        467     337
 
BtsI GCAGTG_nn'

Cuts at:      0     447     648     804 
   Size:        447     201     156

  Fragments arranged by size: 

                 447     201     156
 
DraIII CAC_nnn'GTG

Cuts at:      0     173     804 
   Size:        173     631
 
EaeI y'GGCC_r

Cuts at:      0     428     804 
   Size:        428     376
 
EarI CTCTTCn'nnn_

Cuts at:      0     320     804 
   Size:        320     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     735     804 
   Size:        541     194      69

  Fragments arranged by size: 

                 541     194      69
 
HaeII r_GCGC'y

Cuts at:      0     518     804 
   Size:        518     286
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     445     477     675     707     804 
   Size:        268      32     145      32     198      32      97

  Fragments arranged by size: 

                 268     198     145      97      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     346     804 
   Size:        346     458
 
KasI G'GCGC_C

Cuts at:      0     514     804 
   Size:        514     290
 
MscI TGG'CCA

Cuts at:      0     430     804 
   Size:        430     374
 
MslI CAynn'nnrTG

Cuts at:      0     169     452     707     773     804 
   Size:        169     283     255      66      31

  Fragments arranged by size: 

                 283     255     169      66      31
 
MspA1I CmG'CkG

Cuts at:      0     184     444     479     804 
   Size:        184     260      35     325

  Fragments arranged by size: 

                 325     260     184      35
 
NarI GG'CG_CC

Cuts at:      0     515     804 
   Size:        515     289
 
NsiI A_TGCA'T

Cuts at:      0     530     804 
   Size:        530     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     590     804 
   Size:        590     214
 
PshAI GACnn'nnGTC

Cuts at:      0     339     804 
   Size:        339     465
 
PstI C_TGCA'G

Cuts at:      0     698     804 
   Size:        698     106
 
PvuII CAG'CTG

Cuts at:      0     444     479     804 
   Size:        444      35     325

  Fragments arranged by size: 

                 444     325      35
 
SfcI C'TryA_G

Cuts at:      0      54     337     694     804 
   Size:         54     283     357     110

  Fragments arranged by size: 

                 357     283     110      54
 
SfoI GGC'GCC

Cuts at:      0     516     804 
   Size:        516     288
 
StyI C'CwwG_G

Cuts at:      0     287     804 
   Size:        287     517
 
XbaI T'CTAG_A

Cuts at:      0     463     804 
   Size:        463     341
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     208     804 
   Size:        208     596
 
XmnI GAAnn'nnTTC

Cuts at:      0      28     380     804 
   Size:         28     352     424

  Fragments arranged by size: 

                 424     352      28
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     BanI    BanII     BbeI    BbvCI    BciVI
    BfrBI    BglII     BmrI     BplI     BpmI   Bpu10I    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspEI   BstAPI    BstYI
    BtgZI     BtsI   DraIII     EaeI     EarI  Eco57MI    HaeII    Hin4I
  HindIII     KasI     MscI     MslI   MspA1I     NarI     NsiI    PflMI
    PshAI     PstI    PvuII     SfcI     SfoI     StyI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI     BcgI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI
     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XhoI     XmaI     ZraI
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