Restriction map for pF1KB7542
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AfaI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BbrPI       BbsI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaBI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        MamI        MfeI        MluI        
MluNI       MscI        MunI        NaeI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccIII        173  TCCGGA               T^CCGGA
AcsI          145  GAATTT               [GA]^AATT[CT]
AcyI           25  GGCGCC               G[GA]^CG[CT]C
ApoI          145  GAATTT               [GA]^AATT[CT]
AspHI         523  GAGCTC               G[AT]GC[AT]^C
AvaII          90  GGACC                G^G[AT]CC
BamHI         231  GGATCC               G^GATCC
BanI           25  GGCGCC               G^G[CT][GA]CC
BanII         523  GAGCTC               G[GA]GC[CT]^C
BbeI           25  GGCGCC               GGCGC^C
BcgI          464  GCACTGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          431  TGATCA               T^GATCA
BlpI          520  GCTGAGC              GC^T[ACGT]AGC
BmyI          523  GAGCTC               G[AGT]GC[ACT]^C
BpmI          139  CTGGAG               CTGGAG(16/14)
Bpu1102I      520  GCTGAGC              GC^T[ACGT]AGC
BsaAI         574  TACGTG               [CT]AC^GT[GA]
BsaHI          25  GGCGCC               G[GA]^CG[CT]C
BsaI          613  GAGACC               GGTCTC(1/5)
BsaWI         173  TCCGGA               [AT]^CCGG[AT]
BseAI         173  TCCGGA               T^CCGGA
BseRI         751  GAGGAG               GAGGAG(10/8)
BsgI          196  GTGCAG               GTGCAG(16/14)
BsiHKAI       523  GAGCTC               G[AT]GC[AT]^C
BsmAI         613  GAGAC                GTCTC(1/5)
Bsp1286I      523  GAGCTC               G[AGT]GC[ACT]^C
BspEI         173  TCCGGA               T^CCGGA
BspMI         644  ACCTGC               ACCTGC(4/8)
BstXI         221  CCATTGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         520  GCTGAGC              GC^T[ACGT]AGC
DraII         680  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        538  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          715  CTCTTC               CTCTTC(1/4)
EcoNI         645  CCTGCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      680  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI           11  GCGGG                CCCGC(4/6)
FbaI          431  TGATCA               T^GATCA
HapII         174  CCGG                 C^CGG
Hin1I          25  GGCGCC               G[GA]^CG[CT]C
HinfI         180  GAATC                G^A[ACGT]TC
HpaII         174  CCGG                 C^CGG
KasI           25  GGCGCC               G^GCGCC
Ksp632I       715  CTCTTC               CTCTTC(1/4)
KspI            9  CCGCGG               CCGC^GG
MaeII         575  ACGT                 A^CGT
MaeIII        577  GTGAC                ^GT[ACGT]AC
MroI          173  TCCGGA               T^CCGGA
MseI          605  TTAA                 T^TAA
MspI          174  CCGG                 C^CGG
NarI           25  GGCGCC               GG^CGCC
NheI           96  GCTAGC               G^CTAGC
PstI          550  CTGCAG               CTGCA^G
SacI          523  GAGCTC               GAGCT^C
SacII           9  CCGCGG               CCGC^GG
SfcI          550  CTGCAG               C^T[GA][CT]AG
SfoI           25  GGCGCC               GGC^GCC
TaqI          314  TCGA                 T^CGA
TfiI          180  GAATC                G^A[AT]TC
Tru9I         605  TTAA                 T^TAA
Tsp45I        577  GTGAC                ^GT[GC]AC
TthHB8I       314  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
              626  CGCG                
AflIII        122  ACATGT               A^C[GA][CT]GT
              209  ACATGT              
BbvCI          85  GCTGAGG              CCTCAGC(-5/-2)
              748  GCTGAGG             
BfaI           97  CTAG                 C^TAG
              298  CTAG                
BsmFI          73  GGGAC                GGGAC(10/14)
              281  GGGAC               
BspLU11I      122  ACATGT               A^CATGT
              209  ACATGT              
BstF5I        480  CATCC                GGATG(2/0)
              411  GGATG               
BstUI          10  CGCG                 CG^CG
              626  CGCG                
BstYI         170  AGATCC               [GA]^GATC[CT]
              231  GGATCC              
DsaI            9  CCGCGG               C^C[GA][CT]GG
              542  CCGTGG              
FokI          480  CATCC                GGATG(9/13)
              411  GGATG               
HaeII          25  GGCGCC               [GA]GCGC^[CT]
              327  GGCGCT              
MaeI           97  CTAG                 C^TAG
              298  CTAG                
MflI          170  AGATCC               [GA]^GATC[CT]
              231  GGATCC              
MvnI           10  CGCG                 CG^CG
              626  CGCG                
NlaIII        123  CATG                 CATG^
              210  CATG                
NspI          122  ACATGT               [GA]CATG^[CT]
              209  ACATGT              
Tsp509I       146  AATT                 ^AATT
              309  AATT                
XhoII         170  AGATCC               [GA]^GATC[CT]
              231  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      HhaI        HinP1I      
ItaI        MboI        MboII       MnlI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIV       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
TseI        TspRI       
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