Restriction map for pF1KB7540
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DpnII       DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       FbaI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        MvnI        NdeI        NdeII       
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tru9I       Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          342  GTAC                 GT^AC
AocI          172  CCTCAGG              CC^T[ACGT]AGG
BalI          371  TGGCCA               TGG^CCA
BbeI          157  GGCGCC               GGCGC^C
BglI          131  GCCTCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI          463  CTGCAC               GTGCAG(16/14)
BspMI         303  GCAGGT               ACCTGC(4/8)
BsrBI         358  GAGCGG               CCGCTC(-3/-3)
BsrFI         698  GCCGGC               [GA]^CCGG[CT]
BssSI          23  CTCGTG               CACGAG(-5/-1)
Bsu36I        172  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        698  GCCGGC               [GA]^CCGG[CT]
DraIII         20  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          363  GAAGAG               CTCTTC(1/4)
Eco81I        172  CCTCAGG              CC^T[ACGT]AGG
EcoNI         688  CCTACCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       767  ATGCAT               ATGCA^T
FseI          697  GGCCGGCC             GGCCGG^CC
HaeII         157  GGCGCC               [GA]GCGC^[CT]
HinfI         420  GAATC                G^A[ACGT]TC
HphI          293  TCACC                GGTGA(8/7)
KasI          157  GGCGCC               G^GCGCC
Ksp632I       363  GAAGAG               CTCTTC(1/4)
MaeII         514  ACGT                 A^CGT
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
NaeI          698  GCCGGC               GCC^GGC
NarI          157  GGCGCC               GG^CGCC
NgoMIV        698  GCCGGC               G^CCGGC
NsiI          767  ATGCAT               ATGCA^T
PflMI          19  CCACCTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          538  CTGCAG               CTGCA^G
RsaI          342  GTAC                 GT^AC
SexAI         409  ACCTGGT              A^CC[AT]GGT
SfcI          538  CTGCAG               C^T[GA][CT]AG
SfoI          157  GGCGCC               GGC^GCC
StuI          106  AGGCCT               AGG^CCT
TfiI          420  GAATC                G^A[AT]TC
Van91I         19  CCACCTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BanI          157  GGCGCC               G^G[CT][GA]CC
              558  GGCACC              
BbsI          325  GTCTTC               GAAGAC(2/6)
              516  GTCTTC              
BpmI           42  CTCCAG               CTGGAG(16/14)
              355  CTGGAG              
BpuAI         325  GTCTTC               GAAGAC(2/6)
              516  GTCTTC              
BsaI          482  GGTCTC               GGTCTC(1/5)
              610  GGTCTC              
BseRI           9  CTCCTC               GAGGAG(10/8)
               12  CTCCTC              
BsmAI         483  GTCTC                GTCTC(1/5)
              611  GTCTC               
BsmFI         711  GGGAC                GGGAC(10/14)
              678  GTCCC               
BsrI           62  ACTGG                ACTGG(1/-1)
              339  CCAGT               
BstF5I        152  CATCC                GGATG(2/0)
              765  GGATG               
EaeI          236  TGGCCG               [CT]^GGCC[GA]
              371  TGGCCA              
FokI          152  CATCC                GGATG(9/13)
              765  GGATG               
HgaI          323  GCGTC                GACGC(5/10)
              676  GCGTC               
NcoI          233  CCATGG               C^CATGG
              296  CCATGG              
PpuMI         202  GGGTCCT              [GA]G^G[AT]CC[CT]
              452  AGGACCC             


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcyI        AluI        AlwNI       ApaI        
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsoBI       Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HpaII       ItaI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PvuII       Sau96I      ScrFI       SfaNI       
SmaI        StyI        TseI        XmaCI       XmaI        
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