Restriction map for pF1KB7540
 (Linear) (Six-base) MAPSORT of: KIBB7540.seq  Check: 9092  from: 1  to: 774



KIBB7540.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     345     546     582     672     774 
   Size:        345     201      36      90     102

  Fragments arranged by size: 

                 345     201     102      90      36
 
ApaI G_GGCC'C

Cuts at:      0     273     575     734     774 
   Size:        273     302     159      40

  Fragments arranged by size: 

                 302     273     159      40
 
AvaI C'yCGr_G

Cuts at:      0     123     141     161     477     734     774 
   Size:        123      18      20     316     257      40

  Fragments arranged by size: 

                 316     257     123      40      20      18
 
BanI G'GyrC_C

Cuts at:      0     157     558     774 
   Size:        157     401     216

  Fragments arranged by size: 

                 401     216     157
 
BanII G_rGCy'C

Cuts at:      0     170     273     352     575     734     774 
   Size:        170     103      79     223     159      40

  Fragments arranged by size: 

                 223     170     159     103      79      40
 
BbeI G_GCGC'C

Cuts at:      0     161     774 
   Size:        161     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318     509     774 
   Size:        318     191     265

  Fragments arranged by size: 

                 318     265     191
 
BfrBI ATG'CAT

Cuts at:      0     769     774 
   Size:        769       5
 
BglI GCCn_nnn'nGGC

Cuts at:      0     137     774 
   Size:        137     637
 
Bme1580I G_kGCm'C

Cuts at:      0     273     553     561     575     734     774 
   Size:        273     280       8      14     159      40

  Fragments arranged by size: 

                 280     273     159      40      14       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     376     774 
   Size:         27     349     398

  Fragments arranged by size: 

                 398     349      27
 
Bpu10I CC'TnA_GC

Cuts at:      0      84     346     774 
   Size:         84     262     428

  Fragments arranged by size: 

                 428     262      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     488     616     774 
   Size:        488     128     158

  Fragments arranged by size: 

                 488     158     128
 
BsaHI Gr'CG_yC

Cuts at:      0     158     323     676     774 
   Size:        158     165     353      98

  Fragments arranged by size: 

                 353     165     158      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      52      82     774 
   Size:         52      30     692

  Fragments arranged by size: 

                 692      52      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       3     774 
   Size:          3     771
 
BseYI C'CCAG_C

Cuts at:      0     458     498     708     774 
   Size:        458      40     210      66

  Fragments arranged by size: 

                 458     210      66      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     774 
   Size:        448     326
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     273     352     553     561     575     734     774 
   Size:        170     103      79     201       8      14     159      40

  Fragments arranged by size: 

                 201     170     159     103      79      40      14       8
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     294     774 
   Size:        294     480
 
BsrBI CCG'CTC

Cuts at:      0     360     774 
   Size:        360     414
 
BsrFI r'CCGG_y

Cuts at:      0     698     774 
   Size:        698      76
 
BssSI C'ACGA_G

Cuts at:      0      23     774 
   Size:         23     751
 
Bsu36I CC'TnA_GG

Cuts at:      0     173     774 
   Size:        173     601
 
BtgI C'CryG_G

Cuts at:      0      56     233     296     725     774 
   Size:         56     177      63     429      49

  Fragments arranged by size: 

                 429     177      63      56      49
 
DraIII CAC_nnn'GTG

Cuts at:      0      25     774 
   Size:         25     749
 
EaeI y'GGCC_r

Cuts at:      0     236     371     774 
   Size:        236     135     403

  Fragments arranged by size: 

                 403     236     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     358     774 
   Size:        358     416
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     376     774 
   Size:         27     349     398

  Fragments arranged by size: 

                 398     349      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     692     774 
   Size:        692      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     270     453     572     633     730     731     738 
   Size:        203      67     183     119      61      97       1       7

Cuts at:    738     774 
   Size:         36

  Fragments arranged by size: 

                 203     183     119      97      67      61      36       7
                   1
 
FseI GG_CCGG'CC

Cuts at:      0     702     774 
   Size:        702      72
 
HaeII r_GCGC'y

Cuts at:      0     161     774 
   Size:        161     613
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      84     774 
   Size:         52      32     690

  Fragments arranged by size: 

                 690      52      32
 
KasI G'GCGC_C

Cuts at:      0     157     774 
   Size:        157     617
 
MscI TGG'CCA

Cuts at:      0     373     774 
   Size:        373     401
 
MspA1I CmG'CkG

Cuts at:      0      80     462     543     774 
   Size:         80     382      81     231

  Fragments arranged by size: 

                 382     231      81      80
 
NaeI GCC'GGC

Cuts at:      0     700     774 
   Size:        700      74
 
NarI GG'CG_CC

Cuts at:      0     158     774 
   Size:        158     616
 
NcoI C'CATG_G

Cuts at:      0     233     296     774 
   Size:        233      63     478

  Fragments arranged by size: 

                 478     233      63
 
NgoMIV G'CCGG_C

Cuts at:      0     698     774 
   Size:        698      76
 
NsiI A_TGCA'T

Cuts at:      0     771     774 
   Size:        771       3
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      25     774 
   Size:         25     749
 
PpuMI rG'GwC_Cy

Cuts at:      0     203     453     774 
   Size:        203     250     321

  Fragments arranged by size: 

                 321     250     203
 
PspOMI G'GGCC_C

Cuts at:      0     269     571     730     774 
   Size:        269     302     159      44

  Fragments arranged by size: 

                 302     269     159      44
 
PstI C_TGCA'G

Cuts at:      0     542     774 
   Size:        542     232
 
PvuII CAG'CTG

Cuts at:      0      80     462     543     774 
   Size:         80     382      81     231

  Fragments arranged by size: 

                 382     231      81      80
 
SexAI A'CCwGG_T

Cuts at:      0     409     774 
   Size:        409     365
 
SfcI C'TryA_G

Cuts at:      0     538     774 
   Size:        538     236
 
SfoI GGC'GCC

Cuts at:      0     159     774 
   Size:        159     615
 
SmaI CCC'GGG

Cuts at:      0     125     143     163     736     774 
   Size:        125      18      20     573      38

  Fragments arranged by size: 

                 573     125      38      20      18
 
SrfI GCCC'GGGC

Cuts at:      0     143     774 
   Size:        143     631
 
StuI AGG'CCT

Cuts at:      0     108     774 
   Size:        108     666
 
StyI C'CwwG_G

Cuts at:      0     233     296     314     553     774 
   Size:        233      63      18     239     221

  Fragments arranged by size: 

                 239     233     221      63      18
 
XmaI C'CCGG_G

Cuts at:      0     123     141     161     734     774 
   Size:        123      18      20     573      40

  Fragments arranged by size: 

                 573     123      40      20      18
 

 Enzymes that do cut:

    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BbsI    BfrBI
     BglI Bme1580I     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   Bsu36I
     BtgI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FseI
    HaeII    Hin4I     KasI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI    PflMI    PpuMI   PspOMI     PstI    PvuII    SexAI
     SfcI     SfoI     SmaI     SrfI     StuI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MslI     NdeI     NheI     NotI     NruI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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