Restriction map for pF1KB7539
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmFI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MflI        MluI        MroI        
MseI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          370  GAATTC               [GA]^AATT[CT]
ApaI           44  GGGCCC               GGGCC^C
ApoI          370  GAATTC               [GA]^AATT[CT]
AvaI           47  CCCGGG               C^[CT]CG[GA]G
BanI          494  GGCACC               G^G[CT][GA]CC
BcgI          280  CGAGAGCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI         196  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          393  CTGCAC               GTGCAG(16/14)
BsmAI         144  GTCTC                GTCTC(1/5)
BsmBI         143  CGTCTC               CGTCTC(1/5)
BsmI          508  GAATGC               GAATGC(1/-1)
BsoBI          47  CCCGGG               C^[CT]CG[GA]G
BssSI         323  CTCGTG               CACGAG(-5/-1)
BstAPI          8  GCAATGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        213  GGATG                GGATG(2/0)
BstNI         310  CCAGG                CC^[AT]GG
Cac8I         217  GCCTGC               GC[ACGT]^[ACGT]GC
EarI          146  CTCTTC               CTCTTC(1/4)
EcoRI         370  GAATTC               G^AATTC
EcoRII        310  CCAGG                ^CC[AT]GG
FokI          213  GGATG                GGATG(9/13)
HapII          48  CCGG                 C^CGG
HincII        466  GTCAAC               GT[CT]^[GA]AC
HindII        466  GTCAAC               GT[CT]^[GA]AC
HpaII          48  CCGG                 C^CGG
Ksp632I       146  CTCTTC               CTCTTC(1/4)
MamI          196  GATTGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          515  CAATTG               C^AATTG
MspA1I        477  CCGCTG               C[AC]G^C[GT]G
MspI           48  CCGG                 C^CGG
MunI          515  CAATTG               C^AATTG
MvaI          310  CCAGG                CC^[AT]GG
PstI          447  CTGCAG               CTGCA^G
SfaNI         214  GATGC                GCATC(5/9)
SmaI           47  CCCGGG               CCC^GGG
Tsp45I        326  GTGAC                ^GT[GC]AC
TspRI          40  CACTG                CA[GC]TG[ACGT][ACGT]^
XmaCI          47  CCCGGG               C^CCGGG
XmaI           47  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          477  CCGC                 CCGC(-3/-1)
              230  GCGG                
BalI           36  TGGCCA               TGG^CCA
              240  TGGCCA              
BanII          44  GGGCCC               G[GA]GC[CT]^C
              265  GAGCCC              
BcnI           47  CCCGG                CC^[GC]GG
               48  CCGGG               
BsiYI          58  CCTACTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              498  CCTTGCACGGG         
BslI           58  CCTACTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              498  CCTTGCACGGG         
Cfr13I         44  GGGCC                G^G[ACGT]CC
               45  GGCCC               
DpnII          32  GATC                 ^GATC
              202  GATC                
DsaI           72  CCGTGG               C^C[GA][CT]GG
               93  CCGTGG              
EaeI           36  TGGCCA               [CT]^GGCC[GA]
              240  TGGCCA              
EcoT14I       191  CCAAGG               C^C[AT][AT]GG
              251  CCAAGG              
HinfI         175  GAGTC                G^A[ACGT]TC
              487  GAGTC               
HphI          172  GGTGA                GGTGA(8/7)
              421  GGTGA               
MaeIII        326  GTGAC                ^GT[ACGT]AC
              473  GTTAC               
MboI           32  GATC                 ^GATC
              202  GATC                
MboII         147  TCTTC                GAAGA(8/7)
              204  TCTTC               
MluNI          36  TGGCCA               TGG^CCA
              240  TGGCCA              
MscI           36  TGGCCA               TGG^CCA
              240  TGGCCA              
NciI           47  CCCGG                CC^[GC]GG
               48  CCGGG               
NdeII          32  GATC                 ^GATC
              202  GATC                
NlaIII        337  CATG                 CATG^
              433  CATG                
PleI          175  GAGTC                GAGTC(4/5)
              487  GAGTC               
Sau3AI         32  GATC                 ^GATC
              202  GATC                
Sau96I         44  GGGCC                G^G[ACGT]CC
               45  GGCCC               
StyI          191  CCAAGG               C^C[AT][AT]GG
              251  CCAAGG              
Tsp509I       371  AATT                 ^AATT
              516  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

BbvI        BmyI        BsaJI       Bsp1286I    BsrDI       
BsrI        BssKI       Fnu4HI      HaeIII      ItaI        
MnlI        MslI        MwoI        NlaIV       ScrFI       
SfcI        TseI        
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