Restriction map for pF1KB7524
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MroI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacII       SalI        SbfI        ScaI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          229  GAATTT               [GA]^AATT[CT]
ApoI          229  GAATTT               [GA]^AATT[CT]
Asp700I        43  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          698  CTCGGG               C^[CT]CG[GA]G
AviII         494  TGCGCA               TGC^GCA
BalI          134  TGGCCA               TGG^CCA
BamHI         573  GGATCC               G^GATCC
BanII         168  GAGCTC               G[GA]GC[CT]^C
BfaI           91  CTAG                 C^TAG
BsaI          609  GGTCTC               GGTCTC(1/5)
BsgI          247  GTGCAG               GTGCAG(16/14)
BsmAI         610  GTCTC                GTCTC(1/5)
BsmFI          86  GGGAC                GGGAC(10/14)
BsoBI         698  CTCGGG               C^[CT]CG[GA]G
BspHI          80  TCATGA               T^CATGA
CfoI          495  GCGC                 GCG^C
EaeI          134  TGGCCA               [CT]^GGCC[GA]
EarI          369  CTCTTC               CTCTTC(1/4)
FspI          494  TGCGCA               TGC^GCA
HapII         396  CCGG                 C^CGG
HhaI          495  GCGC                 GCG^C
HinP1I        495  GCGC                 G^CGC
HpaII         396  CCGG                 C^CGG
Ksp632I       369  CTCTTC               CTCTTC(1/4)
MaeI           91  CTAG                 C^TAG
MluNI         134  TGGCCA               TGG^CCA
MscI          134  TGGCCA               TGG^CCA
MseI          484  TTAA                 T^TAA
MspI          396  CCGG                 C^CGG
PflMI         653  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI           80  TCATGA               T^CATGA
SacI          168  GAGCTC               GAGCT^C
SapI          368  GCTCTTC              GCTCTTC(1/4)
SexAI         605  ACCAGGT              A^CC[AT]GGT
SfaNI         516  GATGC                GCATC(5/9)
SmlI          331  CTCAAG               C^T[CT][GA]AG
Tru9I         484  TTAA                 T^TAA
Van91I        653  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           43  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspHI         168  GAGCTC               G[AT]GC[AT]^C
              425  GTGCTC              
AvaII          87  GGACC                G^G[AT]CC
              108  GGACC               
BsaJI         173  CCGAGG               C^C[ACGT][ACGT]GG
              697  CCTCGG              
BsiHKAI       168  GAGCTC               G[AT]GC[AT]^C
              425  GTGCTC              
BssSI         422  CTCGTG               CACGAG(-5/-1)
              490  CTCGTG              
BstYI         200  AGATCC               [GA]^GATC[CT]
              573  GGATCC              
Cac8I         157  GCCCGC               GC[ACGT]^[ACGT]GC
              252  GCTTGC              
DraII          86  GGGACCT              [GA]G^G[ACGT]CC[CT]
              107  AGGACCT             
EcoO109I       86  GGGACCT              [GA]G^G[ACGT]CC[CT]
              107  AGGACCT             
FauI          158  CCCGC                CCCGC(4/6)
                7  GCGGG               
HinfI         349  GAGTC                G^A[ACGT]TC
              399  GACTC               
HphI          235  GGTGA                GGTGA(8/7)
              603  TCACC               
MflI          200  AGATCC               [GA]^GATC[CT]
              573  GGATCC              
NlaIII         81  CATG                 CATG^
              258  CATG                
PleI          399  GACTC                GAGTC(4/5)
              349  GAGTC               
PpuMI          86  GGGACCT              [GA]G^G[AT]CC[CT]
              107  AGGACCT             
XcmI          204  CCAGGAAGCCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              606  CCAGGTCTCCTATGG     
XhoII         200  AGATCC               [GA]^GATC[CT]
              573  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BmyI        
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      Tsp509I     TspRI       
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