Restriction map for pF1KB7524
 (Circular) (Six-base) MAPSORT of: pF1KB7524.seq  Check: 5118  from: 1  to: 3860



pF1KB7524  3860 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    898     898 
   Size:       3860
 
Acc65I G'GTAC_C

Cuts at:    876     876 
   Size:       3860
 
AclI AA'CG_TT

Cuts at:   3700    3700 
   Size:       3860
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    241    1685    2117    2864    3132     241 
   Size:       1444     432     747     268     969

  Fragments arranged by size: 

                1444     969     747     432     268
 
AflIII A'CryG_T

Cuts at:   1212    2316    3293    1212 
   Size:       1104     977    1779

  Fragments arranged by size: 

                1779    1104     977
 
AgeI A'CCGG_T

Cuts at:   2248    2248 
   Size:       3860
 
AlwNI CAG_nnn'CTG

Cuts at:   1165    2732    1165 
   Size:       1567    2293
 
ApaLI G'TGCA_C

Cuts at:   2630    2630 
   Size:       3860
 
ApoI r'AATT_y

Cuts at:    337     864    3213    3469     337 
   Size:        527    2349     256     728

  Fragments arranged by size: 

                2349     728     527     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3860
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3860
 
AvaI C'yCGr_G

Cuts at:    806     880     806 
   Size:         74    3786
 
BamHI G'GATC_C

Cuts at:    681     885     681 
   Size:        204    3656
 
BanI G'GyrC_C

Cuts at:    876    1290    1538    1573     876 
   Size:        414     248      35    3163

  Fragments arranged by size: 

                3163     414     248      35
 
BanII G_rGCy'C

Cuts at:    280     874    1904     280 
   Size:        594    1030    2236

  Fragments arranged by size: 

                2236    1030     594
 
BbeI G_GCGC'C

Cuts at:   1294    1542    1294 
   Size:        248    3612
 
BciVI GTATCCnnnnn_n'

Cuts at:   1758    2519    3860    1758 
   Size:        761    1341    1758

  Fragments arranged by size: 

                1758    1341     761
 
BclI T'GATC_A

Cuts at:   1380    1380 
   Size:       3860
 
BglII A'GATC_T

Cuts at:   1375    3412    1375 
   Size:       2037    1823
 
BlpI GC'TnA_GC

Cuts at:    972     972 
   Size:       3860
 
Bme1580I G_kGCm'C

Cuts at:    267    1485    1578    2634     267 
   Size:       1218      93    1056    1493

  Fragments arranged by size: 

                1493    1218    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1072    1153    1488    3632    1072 
   Size:         81     335    2144    1300

  Fragments arranged by size: 

                2144    1300     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2227    2227 
   Size:       3860
 
Bpu10I CC'TnA_GC

Cuts at:    274     274 
   Size:       3860
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    424    1036    1915    2407    2705     424 
   Size:        612     879     492     298    1579

  Fragments arranged by size: 

                1579     879     612     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    723    3564     723 
   Size:       2841    1019
 
BsaAI yAC'GTr

Cuts at:   1213    1213 
   Size:       3860
 
BsaBI GATnn'nnATC

Cuts at:      6    1374    3411       6 
   Size:       1368    2037     455

  Fragments arranged by size: 

                2037    1368     455
 
BsaHI Gr'CG_yC

Cuts at:   1291    1539    2241    3737    1291 
   Size:        248     702    1496    1414

  Fragments arranged by size: 

                1496    1414     702     248
 
BsaWI w'CCGG_w

Cuts at:   1049    1570    2248    2522    2669    3080    1049 
   Size:        521     678     274     147     411    1829

  Fragments arranged by size: 

                1829     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    703     733     703 
   Size:         30    3830
 
BseYI C'CCAG_C

Cuts at:   1285    2043    2620    3116    1285 
   Size:        758     577     496    2029

  Fragments arranged by size: 

                2029     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    376     376 
   Size:       3860
 
BsiEI CG_ry'CG

Cuts at:    104    1402    1448    2656     104 
   Size:       1298      46    1208    1308

  Fragments arranged by size: 

                1308    1298    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    280     537     874    1652    1842    2634     280 
   Size:        257     337     778     190     792    1506

  Fragments arranged by size: 

                1506     792     778     337     257     190
 
Bsp1286I G_dGCh'C

Cuts at:    267     280     537     874    1485    1578    1652    1842    1904 
   Size:         13     257     337     611      93      74     190      62

Cuts at:   1904    2634     267 
   Size:        730    1493

  Fragments arranged by size: 

                1493     730     611     337     257     190      93      74
                  62      13
 
BspEI T'CCGG_A

Cuts at:   3080    3080 
   Size:       3860
 
BspHI T'CATG_A

Cuts at:    188    3036     188 
   Size:       2848    1012
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    910    1124    1426    1807     910 
   Size:        214     302     381    2963

  Fragments arranged by size: 

                2963     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2152    2206    3690    3855      50 
   Size:       2102      54    1484     165      55

  Fragments arranged by size: 

                2102    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1772    1772 
   Size:       3860
 
BsrFI r'CCGG_y

Cuts at:   1858    2039    2248    1858 
   Size:        181     209    3470

  Fragments arranged by size: 

                3470     209     181
 
BssSI C'ACGA_G

Cuts at:    530     598    2131    2489     530 
   Size:         68    1533     358    1901

  Fragments arranged by size: 

                1901    1533     358      68
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1237    1237 
   Size:       3860
 
BstBI TT'CG_AA

Cuts at:   2221    2221 
   Size:       3860
 
BstYI r'GATC_y

Cuts at:      1     308     681     885    1352    1375    1710    1956    2968 
   Size:        307     373     204     467      23     335     246    1012

Cuts at:   2968    3054    3066    3412       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     373     346     335     307     246
                 204      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3860
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1810    1991    2154    1810 
   Size:        181     163    3516

  Fragments arranged by size: 

                3516     181     163
 
BtsI GCAGTG_nn'

Cuts at:    651    1103     651 
   Size:        452    3408
 
DraI TTT'AAA

Cuts at:    859     859 
   Size:       3860
 
DrdI GACnn_nn'nnGTC

Cuts at:   1566    2424    1566 
   Size:        858    3002
 
EaeI y'GGCC_r

Cuts at:    242    1445    1619    2010    2037    3382     242 
   Size:       1203     174     391      27    1345     720

  Fragments arranged by size: 

                1345    1203     720     391     174      27
 
EagI C'GGCC_G

Cuts at:   1445    1445 
   Size:       3860
 
EarI CTCTTCn'nnn_

Cuts at:    483    1883    2093     483 
   Size:       1400     210    2250

  Fragments arranged by size: 

                2250    1400     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2068    2378    2524    3670    2068 
   Size:        310     146    1146    2258

  Fragments arranged by size: 

                2258    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    278     872     278 
   Size:        594    3266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    241    1685    2117    2864    3132     241 
   Size:       1444     432     747     268     969

  Fragments arranged by size: 

                1444     969     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    195     216     999     195 
   Size:         21     783    3056

  Fragments arranged by size: 

                3056     783      21
 
EcoRI G'AATT_C

Cuts at:    864     864 
   Size:       3860
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1879    1879 
   Size:       3860
 
FspI TGC'GCA

Cuts at:    604    1641     604 
   Size:       1037    2823
 
FspAI rTGC'GCAy

Cuts at:    604     604 
   Size:       3860
 
HaeII r_GCGC'y

Cuts at:   1294    1542    2564    3543    1294 
   Size:        248    1022     979    1611

  Fragments arranged by size: 

                1611    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1702    1732    1734    1764    1702 
   Size:         30       2      30    3798

  Fragments arranged by size: 

                3798      30      30       2
 
HincII GTy'rAC

Cuts at:    899     899 
   Size:       3860
 
KasI G'GCGC_C

Cuts at:   1290    1538    1290 
   Size:        248    3612
 
KpnI G_GTAC'C

Cuts at:    880     880 
   Size:       3860
 
MluI A'CGCG_T

Cuts at:   3293    3293 
   Size:       3860
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    647    2531    2715     647 
   Size:       1884     184    1792

  Fragments arranged by size: 

                1884    1792     184
 
MscI TGG'CCA

Cuts at:    244    1621     244 
   Size:       1377    2483
 
MslI CAynn'nnrTG

Cuts at:   1849    1976    1849 
   Size:        127    3733
 
MspA1I CmG'CkG

Cuts at:    971    1285    1645    2658    2903     971 
   Size:        314     360    1013     245    1928

  Fragments arranged by size: 

                1928    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2041    2041 
   Size:       3860
 
NarI GG'CG_CC

Cuts at:   1291    1539    1291 
   Size:        248    3612
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3860
 
NgoMIV G'CCGG_C

Cuts at:   2039    2039 
   Size:       3860
 
NspI r_CATG'y

Cuts at:    913    1413    2320     913 
   Size:        500     907    2453

  Fragments arranged by size: 

                2453     907     500
 
PciI A'CATG_T

Cuts at:   2316    2316 
   Size:       3860
 
PflMI CCAn_nnn'nTGG

Cuts at:    767     767 
   Size:       3860
 
PfoI T'CCnGG_A

Cuts at:    311     311 
   Size:       3860
 
PmeI GTTT'AAAC

Cuts at:    859     859 
   Size:       3860
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2999    3031    2999 
   Size:         32    3828
 
PpuMI rG'GwC_Cy

Cuts at:    195     216     195 
   Size:         21    3839
 
PstI C_TGCA'G

Cuts at:    907    1592     907 
   Size:        685    3175
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3860
 
PvuII CAG'CTG

Cuts at:   1285    1645    1285 
   Size:        360    3500
 
RsrII CG'GwC_CG

Cuts at:   2055    2055 
   Size:       3860
 
SacI G_AGCT'C

Cuts at:    280     874     280 
   Size:        594    3266
 
SalI G'TCGA_C

Cuts at:    897     897 
   Size:       3860
 
SapI GCTCTTCn'nnn_

Cuts at:    483    1883    2093     483 
   Size:       1400     210    2250

  Fragments arranged by size: 

                2250    1400     210
 
SbfI CC_TGCA'GG

Cuts at:    907     907 
   Size:       3860
 
ScaI AGT'ACT

Cuts at:   3377    3377 
   Size:       3860
 
SexAI A'CCwGG_T

Cuts at:    713     713 
   Size:       3860
 
SfcI C'TryA_G

Cuts at:     33     903    1588    2581    2772      33 
   Size:        870     685     993     191    1121

  Fragments arranged by size: 

                1121     993     870     685     191
 
SfoI GGC'GCC

Cuts at:   1292    1540    1292 
   Size:        248    3612
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3860
 
SmaI CCC'GGG

Cuts at:    882     882 
   Size:       3860
 
SmlI C'TyrA_G

Cuts at:    439    1015    1930    2422    2684     439 
   Size:        576     915     492     262    1615

  Fragments arranged by size: 

                1615     915     576     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3860
 
SphI G_CATG'C

Cuts at:    913    1413     913 
   Size:        500    3360
 
StyI C'CwwG_G

Cuts at:    107     994    1348     107 
   Size:        887     354    2619

  Fragments arranged by size: 

                2619     887     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1443    1576    2035    2244    1443 
   Size:        133     459     209    3059

  Fragments arranged by size: 

                3059     459     209     133
 
TatI w'GTAC_w

Cuts at:   3375    3375 
   Size:       3860
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    703     735    3377    3409     703 
   Size:         32    2642      32    1154

  Fragments arranged by size: 

                2642    1154      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1657    1657 
   Size:       3860
 
XbaI T'CTAG_A

Cuts at:    891     891 
   Size:       3860
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    319     721    1355     319 
   Size:        402     634    2824

  Fragments arranged by size: 

                2824     634     402
 
XmaI C'CCGG_G

Cuts at:    880     880 
   Size:       3860
 
XmnI GAAnn'nnTTC

Cuts at:    155     155 
   Size:       3860
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI    BseRI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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