Restriction map for pF1KB7516
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HaeIII      HgaI        
Hin1I       HindIII     ItaI        KasI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI           80  GTCGAC               GT^[AC][GT]AC
AclI          487  AACGTT               AA^CGTT
Alw44I         63  GTGCAC               G^TGCAC
AlwI          684  GGATC                GGATC(4/5)
ApaLI          63  GTGCAC               G^TGCAC
AspHI          63  GTGCAC               G[AT]GC[AT]^C
BbsI          546  GAAGAC               GAAGAC(2/6)
BbvCI         727  GCTGAGG              CCTCAGC(-5/-2)
BpmI           50  CTGGAG               CTGGAG(16/14)
BpuAI         546  GAAGAC               GAAGAC(2/6)
BseRI         730  GAGGAG               GAGGAG(10/8)
BsiHKAI        63  GTGCAC               G[AT]GC[AT]^C
BspMI         321  GCAGGT               ACCTGC(4/8)
BsrFI          67  ACCGGC               [GA]^CCGG[CT]
BsrI          563  ACTGG                ACTGG(1/-1)
BssKI          39  CCTGG                ^CC[ACGT]GG
BstNI          39  CCTGG                CC^[AT]GG
BstYI         684  GGATCT               [GA]^GATC[CT]
CfoI           87  GCGC                 GCG^C
Cfr10I         67  ACCGGC               [GA]^CCGG[CT]
DpnII         685  GATC                 ^GATC
EarI          334  GAAGAG               CTCTTC(1/4)
EcoRII         39  CCTGG                ^CC[AT]GG
HapII          68  CCGG                 C^CGG
HhaI           87  GCGC                 GCG^C
HinP1I         87  GCGC                 G^CGC
HpaI          444  GTTAAC               GTT^AAC
HpaII          68  CCGG                 C^CGG
Ksp632I       334  GAAGAG               CTCTTC(1/4)
MboI          685  GATC                 ^GATC
MflI          684  GGATCT               [GA]^GATC[CT]
MspI           68  CCGG                 C^CGG
MvaI           39  CCTGG                CC^[AT]GG
NdeII         685  GATC                 ^GATC
PleI          580  GACTC                GAGTC(4/5)
Psp1406I      487  AACGTT               AA^CGTT
SalI           80  GTCGAC               G^TCGAC
Sau3AI        685  GATC                 ^GATC
ScrFI          39  CCTGG                CC^[ACGT]GG
SfcI          230  CTACAG               C^T[GA][CT]AG
SphI          606  GCATGC               GCATG^C
TspRI         449  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         684  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI          532  CCGC                 CCGC(-3/-1)
              313  GCGG                
BmyI           35  GTGCCC               G[AGT]GC[ACT]^C
               63  GTGCAC              
BsiYI          38  CCCTGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              254  CCCAGCGTTGG         
BslI           38  CCCTGGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              254  CCCAGCGTTGG         
Bsp1286I       35  GTGCCC               G[AGT]GC[ACT]^C
               63  GTGCAC              
Cac8I          26  GCGTGC               GC[ACGT]^[ACGT]GC
              606  GCATGC              
HincII         80  GTCGAC               GT[CT]^[GA]AC
              444  GTTAAC              
HindII         80  GTCGAC               GT[CT]^[GA]AC
              444  GTTAAC              
HphI          523  GGTGA                GGTGA(8/7)
              529  TCACC               
MaeII         488  ACGT                 A^CGT
              693  ACGT                
NspI           17  GCATGT               [GA]CATG^[CT]
              606  GCATGC              
SfaNI         220  GATGC                GCATC(5/9)
              301  GATGC               
TaqI           81  TCGA                 T^CGA
              174  TCGA                
TthHB8I        81  TCGA                 T^CGA
              174  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        BsaJI       DdeI        DraI        HinfI       
MboII       MnlI        MseI        MwoI        NlaIII      
TfiI        Tru9I       Tsp509I     
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