Restriction map for pF1KB7514
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvrII       BalI        
BamHI       BanI        BbeI        BcgI        BciVI       
BclI        BfrI        BlnI        BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AfaI          257  GTAC                 GT^AC
AocI          185  CCTCAGG              CC^T[ACGT]AGG
Asp700I       294  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          371  CTCGGG               C^[CT]CG[GA]G
AvaII         216  GGACC                G^G[AT]CC
AviII         524  TGCGCA               TGC^GCA
BbrPI         504  CACGTG               CAC^GTG
BbsI          647  GTCTTC               GAAGAC(2/6)
BbvCI           7  GCTGAGG              CCTCAGC(-5/-2)
BcnI          169  CCCGG                CC^[GC]GG
BfaI          199  CTAG                 C^TAG
BglI          463  GCCAGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           78  GCTGAGC              GC^T[ACGT]AGC
BpmI          289  CTGGAG               CTGGAG(16/14)
Bpu1102I       78  GCTGAGC              GC^T[ACGT]AGC
BpuAI         647  GTCTTC               GAAGAC(2/6)
BsaAI         504  CACGTG               [CT]AC^GT[GA]
BsgI          376  GTGCAG               GTGCAG(16/14)
BsmFI         640  GTCCC                GGGAC(10/14)
BsoBI         371  CTCGGG               C^[CT]CG[GA]G
BsrI          464  CCAGT                ACTGG(1/-1)
BssSI         543  CACGAG               CACGAG(-5/-1)
BstXI         479  CCACAAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        185  CCTCAGG              CC^T[ACGT]AGG
CelII          78  GCTGAGC              GC^T[ACGT]AGC
DsaI          622  CCATGG               C^C[GA][CT]GG
Eco81I        185  CCTCAGG              CC^T[ACGT]AGG
EcoNI         637  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       622  CCATGG               C^C[AT][AT]GG
EcoT22I       681  ATGCAT               ATGCA^T
FspI          524  TGCGCA               TGC^GCA
HapII         170  CCGG                 C^CGG
HinfI         393  GAATC                G^A[ACGT]TC
HpaII         170  CCGG                 C^CGG
MaeI          199  CTAG                 C^TAG
MslI          669  CATCCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          170  CCGG                 C^CGG
NciI          169  CCCGG                CC^[GC]GG
NcoI          622  CCATGG               C^CATGG
NsiI          681  ATGCAT               ATGCA^T
PmaCI         504  CACGTG               CAC^GTG
PmlI          504  CACGTG               CAC^GTG
PstI           27  CTGCAG               CTGCA^G
RsaI          257  GTAC                 GT^AC
SapI          237  GAAGAGC              GCTCTTC(1/4)
SfcI           27  CTGCAG               C^T[GA][CT]AG
SmlI          563  CTCAAG               C^T[CT][GA]AG
StuI          115  AGGCCT               AGG^CCT
StyI          622  CCATGG               C^C[AT][AT]GG
TfiI          393  GAATC                G^A[AT]TC
XmnI          294  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BglII         296  AGATCT               A^GATCT
              380  AGATCT              
BstYI         296  AGATCT               [GA]^GATC[CT]
              380  AGATCT              
CfoI          525  GCGC                 GCG^C
              616  GCGC                
EarI          160  GAAGAG               CTCTTC(1/4)
              237  GAAGAG              
HhaI          525  GCGC                 GCG^C
              616  GCGC                
HinP1I        525  GCGC                 G^CGC
              616  GCGC                
Ksp632I       160  GAAGAG               CTCTTC(1/4)
              237  GAAGAG              
MaeII         323  ACGT                 A^CGT
              505  ACGT                
MflI          296  AGATCT               [GA]^GATC[CT]
              380  AGATCT              
NlaIII        283  CATG                 CATG^
              623  CATG                
XcmI          613  CCAGCGCACCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              697  CCACACCCCAAATGG     
XhoII         296  AGATCT               [GA]^GATC[CT]
              380  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
BanII       BbvI        BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HphI        ItaI        MboI        MboII       
MnlI        MspA1I      MvaI        MwoI        NdeII       
NlaIV       PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        TspRI       
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