Restriction map for pF1KB7449
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        Tru9I       TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI          421  CCGC                 CCGC(-3/-1)
AcsI          121  AAATTC               [GA]^AATT[CT]
AcyI           13  GACGCC               G[GA]^CG[CT]C
AocI          547  CCTGAGG              CC^T[ACGT]AGG
ApoI          121  AAATTC               [GA]^AATT[CT]
Asp700I       359  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          337  CCCGAG               C^[CT]CG[GA]G
BbvI          516  GCAGC                GCAGC(8/12)
BcnI          424  CCGGG                CC^[GC]GG
BfaI          181  CTAG                 C^TAG
BglII         460  AGATCT               A^GATCT
BmyI          417  GTGCCC               G[AGT]GC[ACT]^C
BpmI          310  CTCCAG               CTGGAG(16/14)
BsaHI          13  GACGCC               G[GA]^CG[CT]C
BsaI           27  GAGACC               GGTCTC(1/5)
BsmBI           8  GAGACG               CGTCTC(1/5)
BsoBI         337  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      417  GTGCCC               G[AGT]GC[ACT]^C
BssSI         454  CACGAG               CACGAG(-5/-1)
BstBI         366  TTCGAA               TT^CGAA
BstYI         460  AGATCT               [GA]^GATC[CT]
Bsu36I        547  CCTGAGG              CC^T[ACGT]AGG
DsaI          267  CCGTGG               C^C[GA][CT]GG
Eco81I        547  CCTGAGG              CC^T[ACGT]AGG
FauI          420  CCCGC                CCCGC(4/6)
Fnu4HI        516  GCAGC                GC^[ACGT]GC
HapII         424  CCGG                 C^CGG
HgaI           13  GACGC                GACGC(5/10)
Hin1I          13  GACGCC               G[GA]^CG[CT]C
HpaII         424  CCGG                 C^CGG
ItaI          516  GCAGC                GC^[ACGT]GC
MaeI          181  CTAG                 C^TAG
MflI          460  AGATCT               [GA]^GATC[CT]
MspI          424  CCGG                 C^CGG
NciI          424  CCGGG                CC^[GC]GG
NdeI          148  CATATG               CA^TATG
NspI          483  ACATGC               [GA]CATG^[CT]
NspV          366  TTCGAA               TT^CGAA
PleI          232  GACTC                GAGTC(4/5)
SfuI          366  TTCGAA               TT^CGAA
StuI           68  AGGCCT               AGG^CCT
TseI          516  GCAGC                G^C[AT]GC
Tsp45I        193  GTCAC                ^GT[GC]AC
Tsp509I       122  AATT                 ^AATT
XhoII         460  AGATCT               [GA]^GATC[CT]
XmnI          359  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaII         466  GGACC                G^G[AT]CC
              491  GGACC               
BsiYI         469  CCCAAAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              470  CCAAAAGGGGG         
BslI          469  CCCAAAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              470  CCAAAAGGGGG         
BsmAI           8  GAGAC                GTCTC(1/5)
               27  GAGAC               
BstF5I        321  CATCC                GGATG(2/0)
               36  GGATG               
BstNI          32  CCAGG                CC^[AT]GG
              488  CCTGG               
EcoRII         32  CCAGG                ^CC[AT]GG
              488  CCTGG               
FokI          321  CATCC                GGATG(9/13)
               36  GGATG               
HphI          522  GGTGA                GGTGA(8/7)
              194  TCACC               
MaeII         191  ACGT                 A^CGT
              285  ACGT                
MaeIII        193  GTCAC                ^GT[ACGT]AC
              263  GTAAC               
MspA1I        138  CAGCTG               C[AC]G^C[GT]G
              517  CAGCTG              
MvaI           32  CCAGG                CC^[AT]GG
              488  CCTGG               
MwoI          336  GCCCGAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              414  GCTGTGCCCGC         
PvuII         138  CAGCTG               CAG^CTG
              517  CAGCTG              
TaqI           74  TCGA                 T^CGA
              367  TCGA                
TthHB8I        74  TCGA                 T^CGA
              367  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BcgI        BsaJI       BssKI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        HaeIII      
HinfI       Ksp632I     MboI        MboII       MnlI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        
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