Restriction map for pF1KB7441
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         595  GACGTC               GACGT^C
AccI          172  GTAGAC               GT^[AC][GT]AC
AcyI          595  GACGTC               G[GA]^CG[CT]C
AfaI          149  GTAC                 GT^AC
AflIII         54  ACATGT               A^C[GA][CT]GT
AlwI          387  GGATC                GGATC(4/5)
AseI          613  ATTAAT               AT^TAAT
AsnI          613  ATTAAT               AT^TAAT
AvaII         558  GGTCC                G^G[AT]CC
BclI          546  TGATCA               T^GATCA
BfaI          604  CTAG                 C^TAG
BglII          28  AGATCT               A^GATCT
BpmI          553  CTGGAG               CTGGAG(16/14)
BsaAI         367  TACGTG               [CT]AC^GT[GA]
BsaHI         595  GACGTC               G[GA]^CG[CT]C
BsaI          184  GGTCTC               GGTCTC(1/5)
BsiYI         258  CCAAGCTAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          258  CCAAGCTAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI         624  TCATGA               T^CATGA
BspLU11I       54  ACATGT               A^CATGT
BsrI           85  CCAGT                ACTGG(1/-1)
BstXI          85  CCAGTGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          28  AGATCT               [GA]^GATC[CT]
Cac8I         500  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr13I        558  GGTCC                G^G[ACGT]CC
EarI          226  GAAGAG               CTCTTC(1/4)
FbaI          546  TGATCA               T^GATCA
Hin1I         595  GACGTC               G[GA]^CG[CT]C
Ksp632I       226  GAAGAG               CTCTTC(1/4)
MaeI          604  CTAG                 C^TAG
MboII         226  GAAGA                GAAGA(8/7)
MflI           28  AGATCT               [GA]^GATC[CT]
MspA1I        550  CAGCTG               C[AC]G^C[GT]G
PshBI         613  ATTAAT               AT^TAAT
PvuII         550  CAGCTG               CAG^CTG
RcaI          624  TCATGA               T^CATGA
RsaI          149  GTAC                 GT^AC
SacI          589  GAGCTC               GAGCT^C
Sau96I        558  GGTCC                G^G[ACGT]CC
SmlI          414  CTTGAG               C^T[CT][GA]AG
TaqI          593  TCGA                 T^CGA
TfiI          621  GAATC                G^A[AT]TC
TthHB8I       593  TCGA                 T^CGA
XhoII          28  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI           22  CCGC                 CCGC(-3/-1)
              468  GCGG                
Alw44I          3  GTGCAC               G^TGCAC
               58  GTGCAC              
ApaLI           3  GTGCAC               G^TGCAC
               58  GTGCAC              
BanII          96  GGGCTC               G[GA]GC[CT]^C
              589  GAGCTC              
BsmAI         304  GAGAC                GTCTC(1/5)
              185  GTCTC               
BsrDI         432  CATTGC               GCAATG(2/0)
              471  GCAATG              
BstF5I        255  CATCC                GGATG(2/0)
              531  CATCC               
DsaI          106  CCACGG               C^C[GA][CT]GG
              180  CCACGG              
FokI          255  CATCC                GGATG(9/13)
              531  CATCC               
MaeII         368  ACGT                 A^CGT
              596  ACGT                
MwoI          253  GCCATCCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              511  GCCACCAATGC         
NlaIV         140  GGCTCC               GG[ACGT]^[ACGT]CC
              396  GGAGCC              
PleI          306  GACTC                GAGTC(4/5)
              527  GAGTC               
PstI          310  CTGCAG               CTGCA^G
              498  CTGCAG              
SfcI          310  CTGCAG               C^T[GA][CT]AG
              498  CTGCAG              
XcmI          347  CCAAGAACCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              572  CCATTAAATCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        AspHI       BbvI        BmyI        BsaJI       
BsiHKAI     Bsp1286I    BssKI       BstNI       DdeI        
DpnII       EcoRII      Fnu4HI      HinfI       ItaI        
MaeIII      MboI        MnlI        MseI        MslI        
MvaI        NdeII       NlaIII      NspI        Sau3AI      
ScrFI       Tru9I       TseI        Tsp45I      TspRI       
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