Restriction map for pF1KB7440
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HpaI        KpnI        KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluNI       MroI        
MscI        MseI        MunI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        Sau3AI      
SbfI        SexAI       SfaNI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AcyI          142  GGCGCC               G[GA]^CG[CT]C
AflIII        368  ACGCGT               A^C[GA][CT]GT
AspHI         385  GAGCTC               G[AT]GC[AT]^C
AvaI          146  CCCGAG               C^[CT]CG[GA]G
BanI          142  GGCGCC               G^G[CT][GA]CC
BanII         385  GAGCTC               G[GA]GC[CT]^C
BbeI          142  GGCGCC               GGCGC^C
BbrPI         440  CACGTG               CAC^GTG
BcnI           35  CCCGG                CC^[GC]GG
BfaI          376  CTAG                 C^TAG
BpmI          244  CTGGAG               CTGGAG(16/14)
BsaAI         440  CACGTG               [CT]AC^GT[GA]
BsaHI         142  GGCGCC               G[GA]^CG[CT]C
BsaJI         197  CCATGG               C^C[ACGT][ACGT]GG
BsiHKAI       385  GAGCTC               G[AT]GC[AT]^C
BsiWI          68  CGTACG               C^GTACG
BsmAI         351  GAGAC                GTCTC(1/5)
BsoBI         146  CCCGAG               C^[CT]CG[GA]G
BsrFI         139  GCCGGC               [GA]^CCGG[CT]
BsrI          432  CCAGT                ACTGG(1/-1)
BstF5I        396  CATCC                GGATG(2/0)
BstXI         427  CCAGACCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        139  GCCGGC               [GA]^CCGG[CT]
DraII         239  GGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          197  CCATGG               C^C[GA][CT]GG
EarI          382  GAAGAG               CTCTTC(1/4)
EcoNI          74  CCTTAGTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      239  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       197  CCATGG               C^C[AT][AT]GG
FokI          396  CATCC                GGATG(9/13)
Hin1I         142  GGCGCC               G[GA]^CG[CT]C
HincII         63  GTTGAC               GT[CT]^[GA]AC
HindII         63  GTTGAC               GT[CT]^[GA]AC
HinfI         305  GAGTC                G^A[ACGT]TC
HphI          363  GGTGA                GGTGA(8/7)
KasI          142  GGCGCC               G^GCGCC
Ksp632I       382  GAAGAG               CTCTTC(1/4)
MaeI          376  CTAG                 C^TAG
MboII         382  GAAGA                GAAGA(8/7)
MluI          368  ACGCGT               A^CGCGT
MslI          394  CACATCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          139  GCCGGC               GCC^GGC
NarI          142  GGCGCC               GG^CGCC
NciI           35  CCCGG                CC^[GC]GG
NcoI          197  CCATGG               C^CATGG
NgoMIV        139  GCCGGC               G^CCGGC
NspI          255  GCATGT               [GA]CATG^[CT]
PleI          305  GAGTC                GAGTC(4/5)
PmaCI         440  CACGTG               CAC^GTG
PmlI          440  CACGTG               CAC^GTG
PpuMI         239  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          232  CTGCAG               CTGCA^G
PvuII         183  CAGCTG               CAG^CTG
SacI          385  GAGCTC               GAGCT^C
SapI          382  GAAGAGC              GCTCTTC(1/4)
ScaI           55  AGTACT               AGT^ACT
SfoI          142  GGCGCC               GGC^GCC
SmlI           43  CTTGAG               C^T[CT][GA]AG
StyI          197  CCATGG               C^C[AT][AT]GG
TaqI          329  TCGA                 T^CGA
TspRI         433  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       329  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI           56  GTAC                 GT^AC
               69  GTAC                
AvaII          21  GGACC                G^G[AT]CC
              240  GGACC               
BmyI          385  GAGCTC               G[AGT]GC[ACT]^C
              437  GGGCAC              
BsmFI         239  GGGAC                GGGAC(10/14)
              162  GTCCC               
Bsp1286I      385  GAGCTC               G[AGT]GC[ACT]^C
              437  GGGCAC              
Cfr13I         21  GGACC                G^G[ACGT]CC
              240  GGACC               
DdeI           75  CTTAG                C^T[ACGT]AG
              205  CTCAG               
EaeI          137  TGGCCG               [CT]^GGCC[GA]
              320  CGGCCA              
FauI          390  CCCGC                CCCGC(4/6)
              237  GCGGG               
HaeII         142  GGCGCC               [GA]GCGC^[CT]
              208  AGCGCC              
HaeIII        138  GGCC                 GG^CC
              321  GGCC                
HapII          36  CCGG                 C^CGG
              140  CCGG                
HpaII          36  CCGG                 C^CGG
              140  CCGG                
MaeII          67  ACGT                 A^CGT
              441  ACGT                
MspI           36  CCGG                 C^CGG
              140  CCGG                
RsaI           56  GTAC                 GT^AC
               69  GTAC                
Sau96I         21  GGACC                G^G[ACGT]CC
              240  GGACC               
SfcI          232  CTGCAG               C^T[GA][CT]AG
              294  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BsiYI       
BslI        BssKI       BstNI       BstUI       Cac8I       
CfoI        EcoRII      Fnu4HI      HhaI        HinP1I      
ItaI        MnlI        MspA1I      MvaI        MvnI        
MwoI        NlaIII      NlaIV       ScrFI       TseI        
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