Restriction map for pF1KB7429
 (Linear) (Six-base) MAPSORT of: KIBB7429.seq  Check: 2954  from: 1  to: 897



KIBB7429.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     425     897 
   Size:        425     472
 
AfeI AGC'GCT

Cuts at:      0     772     897 
   Size:        772     125
 
AleI CACnn'nnGTG

Cuts at:      0     541     897 
   Size:        541     356
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     838     897 
   Size:        838      59
 
AlwNI CAG_nnn'CTG

Cuts at:      0     196     897 
   Size:        196     701
 
ApaI G_GGCC'C

Cuts at:      0     595     625     897 
   Size:        595      30     272

  Fragments arranged by size: 

                 595     272      30
 
BanI G'GyrC_C

Cuts at:      0      33     413     762     794     874     897 
   Size:         33     380     349      32      80      23

  Fragments arranged by size: 

                 380     349      80      33      32      23
 
BanII G_rGCy'C

Cuts at:      0     595     625     897 
   Size:        595      30     272

  Fragments arranged by size: 

                 595     272      30
 
BbeI G_GCGC'C

Cuts at:      0      37     766     798     897 
   Size:         37     729      32      99

  Fragments arranged by size: 

                 729      99      37      32
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     859     897 
   Size:        859      38
 
BlpI GC'TnA_GC

Cuts at:      0     106     897 
   Size:        106     791
 
Bme1580I G_kGCm'C

Cuts at:      0     411     595     625     827     877     897 
   Size:        411     184      30     202      50      20

  Fragments arranged by size: 

                 411     202     184      50      30      20
 
BmgBI CAC'GTC

Cuts at:      0     744     834     897 
   Size:        744      90      63

  Fragments arranged by size: 

                 744      90      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     489     598     897 
   Size:        489     109     299

  Fragments arranged by size: 

                 489     299     109
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     126     897 
   Size:        126     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     265     897 
   Size:        250      15     632

  Fragments arranged by size: 

                 632     250      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     523     897 
   Size:        523     374
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     897 
   Size:        634     263
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     700     897 
   Size:        700     197
 
BsaBI GATnn'nnATC

Cuts at:      0     511     897 
   Size:        511     386
 
BsaHI Gr'CG_yC

Cuts at:      0      34     763     795     897 
   Size:         34     729      32     102

  Fragments arranged by size: 

                 729     102      34      32
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153     897 
   Size:        153     744
 
BseYI C'CCAG_C

Cuts at:      0     695     897 
   Size:        695     202
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     612     897 
   Size:        565      47     285

  Fragments arranged by size: 

                 565     285      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     211     897 
   Size:        211     686
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     411     595     625     827     877     897 
   Size:        211     200     184      30     202      50      20

  Fragments arranged by size: 

                 211     202     200     184      50      30      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     425     479     539     806     897 
   Size:        425      54      60     267      91

  Fragments arranged by size: 

                 425     267      91      60      54
 
BsrBI CCG'CTC

Cuts at:      0      45     100     234     334     897 
   Size:         45      55     134     100     563

  Fragments arranged by size: 

                 563     134     100      55      45
 
BsrFI r'CCGG_y

Cuts at:      0      13     709     811     897 
   Size:         13     696     102      86

  Fragments arranged by size: 

                 696     102      86      13
 
BssHII G'CGCG_C

Cuts at:      0     635     897 
   Size:        635     262
 
BssSI C'ACGA_G

Cuts at:      0     274     670     897 
   Size:        274     396     227

  Fragments arranged by size: 

                 396     274     227
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     881     897 
   Size:        881      16
 
Bsu36I CC'TnA_GG

Cuts at:      0      55     690     897 
   Size:         55     635     207

  Fragments arranged by size: 

                 635     207      55
 
BtgI C'CryG_G

Cuts at:      0     212     302     897 
   Size:        212      90     595

  Fragments arranged by size: 

                 595     212      90
 
BtsI GCAGTG_nn'

Cuts at:      0     398     897 
   Size:        398     499
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     111     146     897 
   Size:        111      35     751

  Fragments arranged by size: 

                 751     111      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     497     807     897 
   Size:        497     310      90

  Fragments arranged by size: 

                 497     310      90
 
EaeI y'GGCC_r

Cuts at:      0     641     780     809     897 
   Size:        641     139      29      88

  Fragments arranged by size: 

                 641     139      88      29
 
EarI CTCTTCn'nnn_

Cuts at:      0      35      90     897 
   Size:         35      55     807

  Fragments arranged by size: 

                 807      55      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     265     897 
   Size:        250      15     632

  Fragments arranged by size: 

                 632     250      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     156     897 
   Size:        156     741
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59     193     483     592     621     622     897 
   Size:         59     134     290     109      29       1     275

  Fragments arranged by size: 

                 290     275     134     109      59      29       1
 
HaeII r_GCGC'y

Cuts at:      0      37     766     774     798     897 
   Size:         37     729       8      24      99

  Fragments arranged by size: 

                 729      99      37      24       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     673     705     897 
   Size:        673      32     192

  Fragments arranged by size: 

                 673     192      32
 
KasI G'GCGC_C

Cuts at:      0      33     762     794     897 
   Size:         33     729      32     103

  Fragments arranged by size: 

                 729     103      33      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       8     897 
   Size:          8     889
 
MscI TGG'CCA

Cuts at:      0     643     897 
   Size:        643     254
 
MslI CAynn'nnrTG

Cuts at:      0     296     541     897 
   Size:        296     245     356

  Fragments arranged by size: 

                 356     296     245
 
MspA1I CmG'CkG

Cuts at:      0     214     897 
   Size:        214     683
 
NaeI GCC'GGC

Cuts at:      0      15     813     897 
   Size:         15     798      84

  Fragments arranged by size: 

                 798      84      15
 
NarI GG'CG_CC

Cuts at:      0      34     763     795     897 
   Size:         34     729      32     102

  Fragments arranged by size: 

                 729     102      34      32
 
NgoMIV G'CCGG_C

Cuts at:      0      13     811     897 
   Size:         13     798      86

  Fragments arranged by size: 

                 798      86      13
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     807     897 
   Size:        807      90
 
PfoI T'CCnGG_A

Cuts at:      0     340     897 
   Size:        340     557
 
PpuMI rG'GwC_Cy

Cuts at:      0     483     897 
   Size:        483     414
 
PspOMI G'GGCC_C

Cuts at:      0     591     621     897 
   Size:        591      30     276

  Fragments arranged by size: 

                 591     276      30
 
PstI C_TGCA'G

Cuts at:      0      74     897 
   Size:         74     823
 
SacII CC_GC'GG

Cuts at:      0     215     897 
   Size:        215     682
 
SapI GCTCTTCn'nnn_

Cuts at:      0      35      90     897 
   Size:         35      55     807

  Fragments arranged by size: 

                 807      55      35
 
SfcI C'TryA_G

Cuts at:      0      70     897 
   Size:         70     827
 
SfoI GGC'GCC

Cuts at:      0      35     764     796     897 
   Size:         35     729      32     101

  Fragments arranged by size: 

                 729     101      35      32
 
SmlI C'TyrA_G

Cuts at:      0     649     897 
   Size:        649     248
 
StuI AGG'CCT

Cuts at:      0     151     897 
   Size:        151     746
 
StyI C'CwwG_G

Cuts at:      0      62     314     897 
   Size:         62     252     583

  Fragments arranged by size: 

                 583     252      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     508     897 
   Size:        508     389
 
Tth111I GACn'n_nGTC

Cuts at:      0     883     897 
   Size:        883      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     462     897 
   Size:        462     435
 

 Enzymes that do cut:

     AarI     AfeI     AleI     AlfI    AlwNI     ApaI     BanI    BanII
     BbeI    BciVI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV    PflMI     PfoI    PpuMI   PspOMI
     PstI    SacII     SapI     SfcI     SfoI     SmlI     StuI     StyI
    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BmtI    BsaAI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     ClaI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI     XbaI     XhoI     XmaI     XmnI
     ZraI
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