Restriction map for pF1KB7419
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlnI        BmyI        BpmI        BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        757  GGTACC               G^GTACC
AcsI           31  GAATTC               [GA]^AATT[CT]
ApoI           31  GAATTC               [GA]^AATT[CT]
AseI          520  ATTAAT               AT^TAAT
AsnI          520  ATTAAT               AT^TAAT
Asp718I       757  GGTACC               G^GTACC
AvaII         795  GGACC                G^G[AT]CC
BalI          371  TGGCCA               TGG^CCA
BanI          757  GGTACC               G^G[CT][GA]CC
BbvI          913  GCTGC                GCAGC(8/12)
BglII         539  AGATCT               A^GATCT
BsaAI         103  TACGTA               [CT]AC^GT[GA]
BseRI         225  CTCCTC               GAGGAG(10/8)
BspHI         404  TCATGA               T^CATGA
BsrI          243  ACTGG                ACTGG(1/-1)
BssSI         634  CTCGTG               CACGAG(-5/-1)
BstEII         55  GGTTACC              G^GT[ACGT]ACC
BstPI          55  GGTTACC              G^GT[ACGT]ACC
BstYI         539  AGATCT               [GA]^GATC[CT]
Cfr13I        795  GGACC                G^G[ACGT]CC
DraII         794  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      794  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        55  GGTTACC              G^GT[ACGT]ACC
EcoRI          31  GAATTC               G^AATTC
EcoRV         208  GATATC               GAT^ATC
Fnu4HI        913  GCTGC                GC^[ACGT]GC
HincII        324  GTCAAC               GT[CT]^[GA]AC
HindII        324  GTCAAC               GT[CT]^[GA]AC
ItaI          913  GCTGC                GC^[ACGT]GC
KpnI          757  GGTACC               GGTAC^C
MaeII         104  ACGT                 A^CGT
MflI          539  AGATCT               [GA]^GATC[CT]
MluNI         371  TGGCCA               TGG^CCA
MscI          371  TGGCCA               TGG^CCA
NlaIV         757  GGTACC               GG[ACGT]^[ACGT]CC
PflMI         488  CCATGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           47  GACTC                GAGTC(4/5)
PpuMI         794  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         520  ATTAAT               AT^TAAT
RcaI          404  TCATGA               T^CATGA
Sau96I        795  GGACC                G^G[ACGT]CC
SfaNI         146  GCATC                GCATC(5/9)
SnaBI         103  TACGTA               TAC^GTA
TseI          913  GCTGC                G^C[AT]GC
Tsp45I        859  GTGAC                ^GT[GC]AC
Van91I        488  CCATGCGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          792  CCAGGACCTCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         539  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BbsI          698  GAAGAC               GAAGAC(2/6)
              161  GTCTTC              
BclI          134  TGATCA               T^GATCA
              743  TGATCA              
BlpI          722  GCTCAGC              GC^T[ACGT]AGC
              930  GCTGAGC             
Bpu1102I      722  GCTCAGC              GC^T[ACGT]AGC
              930  GCTGAGC             
BpuAI         698  GAAGAC               GAAGAC(2/6)
              161  GTCTTC              
BsgI          290  GTGCAG               GTGCAG(16/14)
              295  GTGCAG              
BsmFI          45  GGGAC                GGGAC(10/14)
              789  GTCCC               
BsrDI         122  GCAATG               GCAATG(2/0)
              316  GCAATG              
BstF5I        660  CATCC                GGATG(2/0)
              286  GGATG               
Cac8I         142  GCAAGC               GC[ACGT]^[ACGT]GC
              819  GCAAGC              
CelII         722  GCTCAGC              GC^T[ACGT]AGC
              930  GCTGAGC             
EaeI          371  TGGCCA               [CT]^GGCC[GA]
              689  CGGCCA              
EcoT14I       342  CCTTGG               C^C[AT][AT]GG
              801  CCTTGG              
FbaI          134  TGATCA               T^GATCA
              743  TGATCA              
FokI          660  CATCC                GGATG(9/13)
              286  GGATG               
MslI          168  CATGCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              399  CATCATCATG          
NspI          167  ACATGC               [GA]CATG^[CT]
              718  ACATGC              
SfcI          824  CTACAG               C^T[GA][CT]AG
              885  CTATAG              
SmlI           67  CTTGAG               C^T[CT][GA]AG
              891  CTTGAG              
StyI          342  CCTTGG               C^C[AT][AT]GG
              801  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AfaI        AluI        Asp700I     BfaI        BsaJI       
BsiYI       BslI        BssKI       BstNI       DdeI        
DpnII       EcoRII      HaeIII      HinfI       HphI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      RsaI        
Sau3AI      ScrFI       SspI        TfiI        Tru9I       
Tsp509I     TspRI       XmnI        
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