Restriction map for pF1KB7419
 (Linear) (Six-base) MAPSORT of: KIBB7419.seq  Check: 8275  from: 1  to: 954



KIBB7419.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

Acc65I G'GTAC_C

Cuts at:      0     757     954 
   Size:        757     197
 
ApoI r'AATT_y

Cuts at:      0      31     954 
   Size:         31     923
 
AseI AT'TA_AT

Cuts at:      0     521     954 
   Size:        521     433
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     422     455     954 
   Size:        422      33     499

  Fragments arranged by size: 

                 499     422      33
 
BanI G'GyrC_C

Cuts at:      0     757     954 
   Size:        757     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154     705     954 
   Size:        154     551     249

  Fragments arranged by size: 

                 551     249     154
 
BclI T'GATC_A

Cuts at:      0     134     743     954 
   Size:        134     609     211

  Fragments arranged by size: 

                 609     211     134
 
BglII A'GATC_T

Cuts at:      0     539     954 
   Size:        539     415
 
BlpI GC'TnA_GC

Cuts at:      0     723     931     954 
   Size:        723     208      23

  Fragments arranged by size: 

                 723     208      23
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     912     954 
   Size:         88     824      42

  Fragments arranged by size: 

                 824      88      42
 
BsaAI yAC'GTr

Cuts at:      0     105     954 
   Size:        105     849
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     216     954 
   Size:        216     738
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     316     954 
   Size:        311       5     638

  Fragments arranged by size: 

                 638     311       5
 
BspHI T'CATG_A

Cuts at:      0     404     954 
   Size:        404     550
 
BsrDI GCAATG_nn'

Cuts at:      0     129     323     954 
   Size:        129     194     631

  Fragments arranged by size: 

                 631     194     129
 
BssSI C'ACGA_G

Cuts at:      0     634     954 
   Size:        634     320
 
BstEII G'GTnAC_C

Cuts at:      0      55     954 
   Size:         55     899
 
BstYI r'GATC_y

Cuts at:      0     539     954 
   Size:        539     415
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     507     954 
   Size:        507     447
 
BtsI GCAGTG_nn'

Cuts at:      0     299     605     954 
   Size:        299     306     349

  Fragments arranged by size: 

                 349     306     299
 
EaeI y'GGCC_r

Cuts at:      0     371     689     954 
   Size:        371     318     265

  Fragments arranged by size: 

                 371     318     265
 
EcoO109I rG'GnC_Cy

Cuts at:      0     795     954 
   Size:        795     159
 
EcoRI G'AATT_C

Cuts at:      0      31     954 
   Size:         31     923
 
EcoRV GAT'ATC

Cuts at:      0     210     954 
   Size:        210     744
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     383     415     954 
   Size:        383      32     539

  Fragments arranged by size: 

                 539     383      32
 
HincII GTy'rAC

Cuts at:      0     326     954 
   Size:        326     628
 
KpnI G_GTAC'C

Cuts at:      0     761     954 
   Size:        761     193
 
MscI TGG'CCA

Cuts at:      0     373     954 
   Size:        373     581
 
MslI CAynn'nnrTG

Cuts at:      0     172     403     954 
   Size:        172     231     551

  Fragments arranged by size: 

                 551     231     172
 
NspI r_CATG'y

Cuts at:      0     171     722     954 
   Size:        171     551     232

  Fragments arranged by size: 

                 551     232     171
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     494     954 
   Size:        494     460
 
PfoI T'CCnGG_A

Cuts at:      0     203     954 
   Size:        203     751
 
PpuMI rG'GwC_Cy

Cuts at:      0     795     954 
   Size:        795     159
 
SfcI C'TryA_G

Cuts at:      0     824     885     954 
   Size:        824      61      69

  Fragments arranged by size: 

                 824      69      61
 
SmlI C'TyrA_G

Cuts at:      0      67     891     954 
   Size:         67     824      63

  Fragments arranged by size: 

                 824      67      63
 
SnaBI TAC'GTA

Cuts at:      0     105     954 
   Size:        105     849
 
SspI AAT'ATT

Cuts at:      0     516     585     609     925     954 
   Size:        516      69      24     316      29

  Fragments arranged by size: 

                 516     316      69      29      24
 
StyI C'CwwG_G

Cuts at:      0     342     801     954 
   Size:        342     459     153

  Fragments arranged by size: 

                 459     342     153
 
TatI w'GTAC_w

Cuts at:      0     176     423     954 
   Size:        176     247     531

  Fragments arranged by size: 

                 531     247     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     278     310     954 
   Size:        278      32     644

  Fragments arranged by size: 

                 644     278      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     799     954 
   Size:        799     155
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     273     462     954 
   Size:         35     238     189     492

  Fragments arranged by size: 

                 492     238     189      35
 

 Enzymes that do cut:

   Acc65I     ApoI     AseI     BaeI     BanI     BbsI     BclI    BglII
     BlpI    BpuEI    BsaAI    BseRI     BsgI    BspHI    BsrDI    BssSI
   BstEII    BstYI    BtgZI     BtsI     EaeI EcoO109I    EcoRI    EcoRV
    Hin4I   HincII     KpnI     MscI     MslI     NspI    PflMI     PfoI
    PpuMI     SfcI     SmlI    SnaBI     SspI     StyI     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI
     XmaI     ZraI
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