Restriction map for pF1KB7409
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eco47III    EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MslI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          345  GTCTAC               GT^[AC][GT]AC
AfaI          295  GTAC                 GT^AC
AhdI          337  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          488  CCTCAGG              CC^T[ACGT]AGG
AspEI         337  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         555  GTGCTC               G[AT]GC[AT]^C
AvaI           58  CCCGGG               C^[CT]CG[GA]G
AvrII         178  CCTAGG               C^CTAGG
BalI          299  TGGCCA               TGG^CCA
BamHI         125  GGATCC               G^GATCC
BbvI          480  GCAGC                GCAGC(8/12)
BfaI          179  CTAG                 C^TAG
BglI           25  GCCCGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          178  CCTAGG               C^CTAGG
BlpI          145  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      145  GCTGAGC              GC^T[ACGT]AGC
BsaI          458  GAGACC               GGTCTC(1/5)
BsiEI          17  CGGCCG               CG[GA][CT]^CG
BsiHKAI       555  GTGCTC               G[AT]GC[AT]^C
BsmFI          62  GGGAC                GGGAC(10/14)
BsoBI          58  CCCGGG               C^[CT]CG[GA]G
BspMI         272  ACCTGC               ACCTGC(4/8)
BsrBI         520  CCGCTC               CCGCTC(-3/-3)
BstEII        499  GGTGACC              G^GT[ACGT]ACC
BstPI         499  GGTGACC              G^GT[ACGT]ACC
BstXI         129  CCAGACACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         125  GGATCC               [GA]^GATC[CT]
Bsu36I        488  CCTCAGG              CC^T[ACGT]AGG
CelII         145  GCTGAGC              GC^T[ACGT]AGC
DraII         384  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          112  CCGTGG               C^C[GA][CT]GG
EagI           17  CGGCCG               C^GGCCG
Eam1105I      337  GACGATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          370  GAAGAG               CTCTTC(1/4)
EclXI          17  CGGCCG               C^GGCCG
Eco52I         17  CGGCCG               C^GGCCG
Eco81I        488  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      384  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       499  GGTGACC              G^GT[ACGT]ACC
HphI          499  GGTGA                GGTGA(8/7)
Ksp632I       370  GAAGAG               CTCTTC(1/4)
MaeI          179  CTAG                 C^TAG
MflI          125  GGATCC               [GA]^GATC[CT]
MluNI         299  TGGCCA               TGG^CCA
MscI          299  TGGCCA               TGG^CCA
NlaIII        252  CATG                 CATG^
PflMI         411  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         384  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI          295  GTAC                 GT^AC
SapI          370  GAAGAGC              GCTCTTC(1/4)
ScaI          294  AGTACT               AGT^ACT
SmaI           58  CCCGGG               CCC^GGG
StuI           76  AGGCCT               AGG^CCT
TseI          480  GCAGC                G^C[AT]GC
Van91I        411  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          189  CCAACACACTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         125  GGATCC               [GA]^GATC[CT]
XmaCI          58  CCCGGG               C^CCGGG
XmaI           58  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          149  AGCT                 AG^CT
              366  AGCT                
AvaII         385  GGACC                G^G[AT]CC
              517  GGTCC               
BpmI           72  CTGGAG               CTGGAG(16/14)
              137  CTGGAG              
BseRI         537  CTCCTC               GAGGAG(10/8)
              558  CTCCTC              
BsmAI         458  GAGAC                GTCTC(1/5)
              535  GTCTC               
BstF5I        229  CATCC                GGATG(2/0)
              563  CATCC               
Cac8I          92  GCGGGC               GC[ACGT]^[ACGT]GC
              315  GCAAGC              
EaeI           17  CGGCCG               [CT]^GGCC[GA]
              299  TGGCCA              
FokI          229  CATCC                GGATG(9/13)
              563  CATCC               
HgaI           64  GACGC                GACGC(5/10)
              261  GCGTC               
MaeIII         50  GTCAC                ^GT[ACGT]AC
              500  GTGAC               
MboII         370  GAAGA                GAAGA(8/7)
              402  TCTTC               
SmlI          527  CTCAAG               C^T[CT][GA]AG
              540  CTCAAG              
Tsp45I         50  GTCAC                ^GT[GC]AC
              500  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AlwI        BanII       BcnI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstNI       BstUI       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MboI        
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TspRI       
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