Restriction map for pF1KB7409
 (Linear) (Six-base) MAPSORT of: KIBB7409.seq  Check: 5008  from: 1  to: 570



KIBB7409.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     346     570 
   Size:        346     224
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     570 
   Size:        389     181
 
AhdI GACnn_n'nnGTC

Cuts at:      0     342     570 
   Size:        342     228
 
AvaI C'yCGr_G

Cuts at:      0      58     570 
   Size:         58     512
 
AvrII C'CTAG_G

Cuts at:      0     178     570 
   Size:        178     392
 
BamHI G'GATC_C

Cuts at:      0     125     570 
   Size:        125     445
 
BanII G_rGCy'C

Cuts at:      0       8      27     106     570 
   Size:          8      19      79     464

  Fragments arranged by size: 

                 464      79      19       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0      31     570 
   Size:         31     539
 
BlpI GC'TnA_GC

Cuts at:      0     146     570 
   Size:        146     424
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     304     516     570 
   Size:        304     212      54

  Fragments arranged by size: 

                 304     212      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     158     570 
   Size:         93      65     412

  Fragments arranged by size: 

                 412      93      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512     525     570 
   Size:        512      13      45

  Fragments arranged by size: 

                 512      45      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     452     570 
   Size:        452     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     528     549     570 
   Size:        528      21      21

  Fragments arranged by size: 

                 528      21      21
 
BsiEI CG_ry'CG

Cuts at:      0      20     570 
   Size:         20     550
 
BsiHKAI G_wGCw'C

Cuts at:      0     559     570 
   Size:        559      11
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      27     106     559     570 
   Size:          8      19      79     453      11

  Fragments arranged by size: 

                 453      79      19      11       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     281     570 
   Size:        281     289
 
BsrBI CCG'CTC

Cuts at:      0     522     570 
   Size:        522      48
 
BstEII G'GTnAC_C

Cuts at:      0     499     570 
   Size:        499      71
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     136     570 
   Size:        136     434
 
BstYI r'GATC_y

Cuts at:      0     125     570 
   Size:        125     445
 
Bsu36I CC'TnA_GG

Cuts at:      0     489     570 
   Size:        489      81
 
BtgI C'CryG_G

Cuts at:      0     112     570 
   Size:        112     458
 
BtsI GCAGTG_nn'

Cuts at:      0      51     570 
   Size:         51     519
 
EaeI y'GGCC_r

Cuts at:      0      17     299     570 
   Size:         17     282     271

  Fragments arranged by size: 

                 282     271      17
 
EagI C'GGCC_G

Cuts at:      0      17     570 
   Size:         17     553
 
EarI CTCTTCn'nnn_

Cuts at:      0     365     570 
   Size:        365     205
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      71     570 
   Size:         71     499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     158     389     570 
   Size:         93      65     231     181

  Fragments arranged by size: 

                 231     181      93      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     385     570 
   Size:        385     185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     231     570 
   Size:        231     339
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     328     360     570 
   Size:        110      32     186      32     210

  Fragments arranged by size: 

                 210     186     110      32      32
 
MscI TGG'CCA

Cuts at:      0     301     570 
   Size:        301     269
 
PasI CC'CwG_GG

Cuts at:      0     442     570 
   Size:        442     128
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     417     570 
   Size:        417     153
 
PpuMI rG'GwC_Cy

Cuts at:      0     385     570 
   Size:        385     185
 
SapI GCTCTTCn'nnn_

Cuts at:      0     365     570 
   Size:        365     205
 
ScaI AGT'ACT

Cuts at:      0     296     570 
   Size:        296     274
 
SmaI CCC'GGG

Cuts at:      0      60     570 
   Size:         60     510
 
SmlI C'TyrA_G

Cuts at:      0     527     540     570 
   Size:        527      13      30

  Fragments arranged by size: 

                 527      30      13
 
StuI AGG'CCT

Cuts at:      0      78     570 
   Size:         78     492
 
StyI C'CwwG_G

Cuts at:      0     121     178     474     570 
   Size:        121      57     296      96

  Fragments arranged by size: 

                 296     121      96      57
 
TatI w'GTAC_w

Cuts at:      0     294     570 
   Size:        294     276
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     196     570 
   Size:        196     374
 
XmaI C'CCGG_G

Cuts at:      0      58     570 
   Size:         58     512
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AvaI    AvrII    BamHI    BanII     BglI
     BlpI     BmrI     BpmI    BpuEI     BsaI    BseRI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I     FalI
    Hin4I     MscI     PasI    PflMI    PpuMI     SapI     ScaI     SmaI
     SmlI     StuI     StyI     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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