Restriction map for pF1KB7407
 (Linear) (Six-base) MAPSORT of: KIBB7407.seq  Check: 5434  from: 1  to: 639



KIBB7407.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     116     639 
   Size:        116     523
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     639 
   Size:        517     122
 
AfeI AGC'GCT

Cuts at:      0     331     639 
   Size:        331     308
 
AvaI C'yCGr_G

Cuts at:      0     358     579     639 
   Size:        358     221      60

  Fragments arranged by size: 

                 358     221      60
 
BanI G'GyrC_C

Cuts at:      0       3     231     354     639 
   Size:          3     228     123     285

  Fragments arranged by size: 

                 285     228     123       3
 
BanII G_rGCy'C

Cuts at:      0     548     639 
   Size:        548      91
 
BbeI G_GCGC'C

Cuts at:      0       7     235     358     639 
   Size:          7     228     123     281

  Fragments arranged by size: 

                 281     228     123       7
 
BbvCI CC'TCA_GC

Cuts at:      0     601     639 
   Size:        601      38
 
BglI GCCn_nnn'nGGC

Cuts at:      0     320     363     584     639 
   Size:        320      43     221      55

  Fragments arranged by size: 

                 320     221      55      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     542     601     639 
   Size:        542      59      38

  Fragments arranged by size: 

                 542      59      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     639 
   Size:        355     284
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517     639 
   Size:        517     122
 
BsaAI yAC'GTr

Cuts at:      0     267     507     639 
   Size:        267     240     132

  Fragments arranged by size: 

                 267     240     132
 
BsaHI Gr'CG_yC

Cuts at:      0       4      83     232     355     639 
   Size:          4      79     149     123     284

  Fragments arranged by size: 

                 284     149     123      79       4
 
BseYI C'CCAG_C

Cuts at:      0     332     639 
   Size:        332     307
 
BsiEI CG_ry'CG

Cuts at:      0     248     514     639 
   Size:        248     266     125

  Fragments arranged by size: 

                 266     248     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     263     620     639 
   Size:        263     357      19

  Fragments arranged by size: 

                 357     263      19
 
Bsp1286I G_dGCh'C

Cuts at:      0     263     548     620     639 
   Size:        263     285      72      19

  Fragments arranged by size: 

                 285     263      72      19
 
BsrBI CCG'CTC

Cuts at:      0     369     639 
   Size:        369     270
 
BsrFI r'CCGG_y

Cuts at:      0     243     344     479     639 
   Size:        243     101     135     160

  Fragments arranged by size: 

                 243     160     135     101
 
BsrGI T'GTAC_A

Cuts at:      0     272     639 
   Size:        272     367
 
BssHII G'CGCG_C

Cuts at:      0      70      72     639 
   Size:         70       2     567

  Fragments arranged by size: 

                 567      70       2
 
BssSI C'ACGA_G

Cuts at:      0     436     567     639 
   Size:        436     131      72

  Fragments arranged by size: 

                 436     131      72
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     267     639 
   Size:        267     372
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     474     639 
   Size:        474     165
 
BtgI C'CryG_G

Cuts at:      0     350     639 
   Size:        350     289
 
DraIII CAC_nnn'GTG

Cuts at:      0     267     639 
   Size:        267     372
 
EaeI y'GGCC_r

Cuts at:      0     245     432     511     639 
   Size:        245     187      79     128

  Fragments arranged by size: 

                 245     187     128      79
 
EagI C'GGCC_G

Cuts at:      0     245     511     639 
   Size:        245     266     128

  Fragments arranged by size: 

                 266     245     128
 
EarI CTCTTCn'nnn_

Cuts at:      0     400     601     639 
   Size:        400     201      38

  Fragments arranged by size: 

                 400     201      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     639 
   Size:        517     122
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     532     639 
   Size:        532     107
 
HaeII r_GCGC'y

Cuts at:      0       7      18     235     333     358     639 
   Size:          7      11     217      98      25     281

  Fragments arranged by size: 

                 281     217      98      25      11       7
 
HincII GTy'rAC

Cuts at:      0     282     639 
   Size:        282     357
 
KasI G'GCGC_C

Cuts at:      0       3     231     354     639 
   Size:          3     228     123     285

  Fragments arranged by size: 

                 285     228     123       3
 
MslI CAynn'nnrTG

Cuts at:      0     613     639 
   Size:        613      26
 
MspA1I CmG'CkG

Cuts at:      0      21      30      51     237     352     639 
   Size:         21       9      21     186     115     287

  Fragments arranged by size: 

                 287     186     115      21      21       9
 
NaeI GCC'GGC

Cuts at:      0     245     639 
   Size:        245     394
 
NarI GG'CG_CC

Cuts at:      0       4     232     355     639 
   Size:          4     228     123     284

  Fragments arranged by size: 

                 284     228     123       4
 
NgoMIV G'CCGG_C

Cuts at:      0     243     639 
   Size:        243     396
 
PasI CC'CwG_GG

Cuts at:      0     411     639 
   Size:        411     228
 
RsrII CG'GwC_CG

Cuts at:      0     102     639 
   Size:        102     537
 
SacII CC_GC'GG

Cuts at:      0     353     639 
   Size:        353     286
 
SapI GCTCTTCn'nnn_

Cuts at:      0     400     639 
   Size:        400     239
 
SfoI GGC'GCC

Cuts at:      0       5     233     356     639 
   Size:          5     228     123     283

  Fragments arranged by size: 

                 283     228     123       5
 
SmaI CCC'GGG

Cuts at:      0     360     581     639 
   Size:        360     221      58

  Fragments arranged by size: 

                 360     221      58
 
SmlI C'TyrA_G

Cuts at:      0     370     639 
   Size:        370     269
 
SrfI GCCC'GGGC

Cuts at:      0     581     639 
   Size:        581      58
 
TatI w'GTAC_w

Cuts at:      0     272     639 
   Size:        272     367
 
XmaI C'CCGG_G

Cuts at:      0     358     579     639 
   Size:        358     221      60

  Fragments arranged by size: 

                 358     221      60
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AvaI     BanI    BanII     BbeI    BbvCI
     BglI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstXI     BtgI   DraIII     EaeI     EagI     EarI  Eco57MI    EcoNI
    HaeII   HincII     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV
     PasI    RsrII    SacII     SapI     SfoI     SmaI     SmlI     SrfI
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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