Restriction map for pF1KB7405
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AlwI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BbrPI       BbvCI       BclI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BseRI       BsiWI       BsmI        
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     CpoI        DraI        DrdI        
Eam1105I    Eco47III    EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Van91I      XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI         1094  AAATTC               [GA]^AATT[CT]
Alw44I       1003  GTGCAC               G^TGCAC
AlwNI         466  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1300  GGGCCC               GGGCC^C
ApaLI        1003  GTGCAC               G^TGCAC
ApoI         1094  AAATTC               [GA]^AATT[CT]
BanI          185  GGCGCC               G^G[CT][GA]CC
BanII        1300  GGGCCC               G[GA]GC[CT]^C
BbeI          185  GGCGCC               GGCGC^C
BcgI           95  GCAACTCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1056  GTATCC               GTATCC(6/5)
BfaI         1309  CTAG                 C^TAG
BpmI         1171  CTGGAG               CTGGAG(16/14)
BsgI         1045  CTGCAC               GTGCAG(16/14)
BsmBI        1343  GAGACG               CGTCTC(1/5)
Bsp1407I      993  TGTACA               T^GTACA
BspDI         328  ATCGAT               AT^CGAT
BspMI         637  GCAGGT               ACCTGC(4/8)
BsrGI         993  TGTACA               T^GTACA
BstAPI         17  GCAAGACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1137  CATCC                GGATG(2/0)
BstXI         284  CCATCTTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          328  ATCGAT               AT^CGAT
DpnII         144  GATC                 ^GATC
DraIII       1126  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          566  CGGCCG               C^GGCCG
EclXI         566  CGGCCG               C^GGCCG
Eco52I        566  CGGCCG               C^GGCCG
EcoRV        1073  GATATC               GAT^ATC
EcoT14I      1217  CCAAGG               C^C[AT][AT]GG
FokI         1137  CATCC                GGATG(9/13)
KasI          185  GGCGCC               G^GCGCC
KspI          526  CCGCGG               CCGC^GG
MaeI         1309  CTAG                 C^TAG
MboI          144  GATC                 ^GATC
MseI         1409  TTAA                 T^TAA
NarI          185  GGCGCC               GG^CGCC
NdeII         144  GATC                 ^GATC
PstI          454  CTGCAG               CTGCA^G
SacII         526  CCGCGG               CCGC^GG
Sau3AI        144  GATC                 ^GATC
SfoI          185  GGCGCC               GGC^GCC
SspBI         993  TGTACA               T^GTACA
StyI         1217  CCAAGG               C^C[AT][AT]GG
Tru9I        1409  TTAA                 T^TAA
XbaI         1308  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          185  GGCGCC               G[GA]^CG[CT]C
              358  GGCGTC              
AfaI          721  GTAC                 GT^AC
              994  GTAC                
AocI          508  CCTCAGG              CC^T[ACGT]AGG
             1060  CCTAAGG             
AspI          440  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1400  GACAAAGTC           
BbsI          670  GAAGAC               GAAGAC(2/6)
              259  GTCTTC              
BpuAI         670  GAAGAC               GAAGAC(2/6)
              259  GTCTTC              
BsaHI         185  GGCGCC               G[GA]^CG[CT]C
              358  GGCGTC              
BsaI          901  GAGACC               GGTCTC(1/5)
               78  GGTCTC              
BsiEI         566  CGGCCG               CG[GA][CT]^CG
              617  CGACCG              
BsrFI          26  GCCGGC               [GA]^CCGG[CT]
              169  GCCGGC              
BsrI          821  ACTGG                ACTGG(1/-1)
             1140  CCAGT               
BssHII        242  GCGCGC               G^CGCGC
              531  GCGCGC              
Bsu36I        508  CCTCAGG              CC^T[ACGT]AGG
             1060  CCTAAGG             
Cfr10I         26  GCCGGC               [GA]^CCGG[CT]
              169  GCCGGC              
EaeI          566  CGGCCG               [CT]^GGCC[GA]
              773  CGGCCA              
Eco81I        508  CCTCAGG              CC^T[ACGT]AGG
             1060  CCTAAGG             
Hin1I         185  GGCGCC               G[GA]^CG[CT]C
              358  GGCGTC              
NaeI           26  GCCGGC               GCC^GGC
              169  GCCGGC              
NgoMIV         26  GCCGGC               G^CCGGC
              169  GCCGGC              
NspI          128  ACATGC               [GA]CATG^[CT]
              729  GCATGT              
PflFI         440  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1400  GACAAAGTC           
PvuII         469  CAGCTG               CAG^CTG
             1234  CAGCTG              
RsaI          721  GTAC                 GT^AC
              994  GTAC                
SapI          949  GAAGAGC              GCTCTTC(1/4)
               33  GCTCTTC             
SfcI          454  CTGCAG               C^T[GA][CT]AG
              919  CTACAG              
SmaI          194  CCCGGG               CCC^GGG
             1249  CCCGGG              
TaqI          329  TCGA                 T^CGA
             1098  TCGA                
TfiI          643  GATTC                G^A[AT]TC
             1121  GAATC               
Tsp45I       1132  GTGAC                ^GT[GC]AC
             1391  GTGAC               
Tsp509I       984  AATT                 ^AATT
             1095  AATT                
Tth111I       440  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1400  GACAAAGTC           
TthHB8I       329  TCGA                 T^CGA
             1098  TCGA                
XmaCI         194  CCCGGG               C^CCGGG
             1249  CCCGGG              
XmaI          194  CCCGGG               C^CCGGG
             1249  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
AvaII       BbvI        BcnI        BlpI        BmyI        
Bpu1102I    BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DraII       DsaI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
PleI        PpuMI       Sau96I      ScrFI       SfaNI       
TseI        TspRI       
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