Restriction map for pF1KB7405
 (Linear) (Six-base) MAPSORT of: KIBB7405.seq  Check: 1515  from: 1  to: 1455



KIBB7405.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     628    1455 
   Size:        628     827
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     719     820    1455 
   Size:        186     533     101     635

  Fragments arranged by size: 

                 635     533     186     101
 
AleI CACnn'nnGTG

Cuts at:      0    1295    1455 
   Size:       1295     160
 
AlwNI CAG_nnn'CTG

Cuts at:      0     471    1455 
   Size:        471     984
 
ApaI G_GGCC'C

Cuts at:      0    1304    1455 
   Size:       1304     151
 
ApaLI G'TGCA_C

Cuts at:      0    1003    1455 
   Size:       1003     452
 
ApoI r'AATT_y

Cuts at:      0    1094    1455 
   Size:       1094     361
 
AvaI C'yCGr_G

Cuts at:      0     194     319     337    1249    1455 
   Size:        194     125      18     912     206

  Fragments arranged by size: 

                 912     206     194     125      18
 
BanI G'GyrC_C

Cuts at:      0     185    1455 
   Size:        185    1270
 
BanII G_rGCy'C

Cuts at:      0    1304    1455 
   Size:       1304     151
 
BbeI G_GCGC'C

Cuts at:      0     189    1455 
   Size:        189    1266
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     252     677    1455 
   Size:        252     425     778

  Fragments arranged by size: 

                 778     425     252
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84     118    1455 
   Size:         84      34    1337

  Fragments arranged by size: 

                1337      84      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1067    1455 
   Size:       1067     388
 
BlpI GC'TnA_GC

Cuts at:      0     247    1229    1286    1455 
   Size:        247     982      57     169

  Fragments arranged by size: 

                 982     247     169      57
 
Bme1580I G_kGCm'C

Cuts at:      0    1007    1257    1304    1455 
   Size:       1007     250      47     151

  Fragments arranged by size: 

                1007     250     151      47
 
BmgBI CAC'GTC

Cuts at:      0      23    1272    1455 
   Size:         23    1249     183

  Fragments arranged by size: 

                1249     183      23
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      96    1455 
   Size:         96    1359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1192    1455 
   Size:       1192     263
 
Bpu10I CC'TnA_GC

Cuts at:      0     163    1455 
   Size:        163    1292
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      84     895    1455 
   Size:         84     811     560

  Fragments arranged by size: 

                 811     560      84
 
BsaHI Gr'CG_yC

Cuts at:      0     186     359    1455 
   Size:        186     173    1096

  Fragments arranged by size: 

                1096     186     173
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     118     148    1455 
   Size:        118      30    1307

  Fragments arranged by size: 

                1307     118      30
 
BseYI C'CCAG_C

Cuts at:      0     841    1455 
   Size:        841     614
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1030    1455 
   Size:       1030     425
 
BsiEI CG_ry'CG

Cuts at:      0     569     620    1455 
   Size:        569      51     835

  Fragments arranged by size: 

                 835     569      51
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     350    1007    1292    1455 
   Size:         50     300     657     285     163

  Fragments arranged by size: 

                 657     300     285     163      50
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1337    1455 
   Size:       1337     118
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     350    1007    1257    1292    1304    1455 
   Size:         50     300     657     250      35      12     151

  Fragments arranged by size: 

                 657     300     250     151      50      35      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     628    1455 
   Size:        628     827
 
BsrFI r'CCGG_y

Cuts at:      0      26     169    1455 
   Size:         26     143    1286

  Fragments arranged by size: 

                1286     143      26
 
BsrGI T'GTAC_A

Cuts at:      0     993    1455 
   Size:        993     462
 
BssHII G'CGCG_C

Cuts at:      0     242     531    1455 
   Size:        242     289     924

  Fragments arranged by size: 

                 924     289     242
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      23    1455 
   Size:         23    1432
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     291    1455 
   Size:        291    1164
 
Bsu36I CC'TnA_GG

Cuts at:      0     509    1061    1455 
   Size:        509     552     394

  Fragments arranged by size: 

                 552     509     394
 
BtgI C'CryG_G

Cuts at:      0     526     755    1395    1455 
   Size:        526     229     640      60

  Fragments arranged by size: 

                 640     526     229      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      87     162    1455 
   Size:         87      75    1293

  Fragments arranged by size: 

                1293      87      75
 
BtsI GCAGTG_nn'

Cuts at:      0     832     996    1255    1455 
   Size:        832     164     259     200

  Fragments arranged by size: 

                 832     259     200     164
 
ClaI AT'CG_AT

Cuts at:      0     329    1455 
   Size:        329    1126
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1337    1372    1455 
   Size:       1337      35      83

  Fragments arranged by size: 

                1337      83      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1131    1455 
   Size:       1131     324
 
EaeI y'GGCC_r

Cuts at:      0     566     773    1455 
   Size:        566     207     682

  Fragments arranged by size: 

                 682     566     207
 
EagI C'GGCC_G

Cuts at:      0     566    1455 
   Size:        566     889
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     796     944    1335    1455 
   Size:         40     756     148     391     120

  Fragments arranged by size: 

                 756     391     148     120      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85     490     553    1455 
   Size:         85     405      63     902

  Fragments arranged by size: 

                 902     405      85      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     719     820    1192    1455 
   Size:        186     533     101     372     263

  Fragments arranged by size: 

                 533     372     263     186     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0     505     522     584     810    1301    1455 
   Size:        505      17      62     226     491     154

  Fragments arranged by size: 

                 505     491     226     154      62      17
 
EcoRV GAT'ATC

Cuts at:      0    1075    1455 
   Size:       1075     380
 
HaeII r_GCGC'y

Cuts at:      0     170     189     209     497    1455 
   Size:        170      19      20     288     958

  Fragments arranged by size: 

                 958     288     170      20      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     679     711    1455 
   Size:        130      32     517      32     744

  Fragments arranged by size: 

                 744     517     130      32      32
 
HincII GTy'rAC

Cuts at:      0     378     942    1440    1455 
   Size:        378     564     498      15

  Fragments arranged by size: 

                 564     498     378      15
 
KasI G'GCGC_C

Cuts at:      0     185    1455 
   Size:        185    1270
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     161    1455 
   Size:        140      21    1294

  Fragments arranged by size: 

                1294     140      21
 
MslI CAynn'nnrTG

Cuts at:      0      43     289    1274    1295    1455 
   Size:         43     246     985      21     160

  Fragments arranged by size: 

                 985     246     160      43      21
 
MspA1I CmG'CkG

Cuts at:      0     462     471     528     907    1236    1455 
   Size:        462       9      57     379     329     219

  Fragments arranged by size: 

                 462     379     329     219      57       9
 
NaeI GCC'GGC

Cuts at:      0      28     171    1455 
   Size:         28     143    1284

  Fragments arranged by size: 

                1284     143      28
 
NarI GG'CG_CC

Cuts at:      0     186    1455 
   Size:        186    1269
 
NgoMIV G'CCGG_C

Cuts at:      0      26     169    1455 
   Size:         26     143    1286

  Fragments arranged by size: 

                1286     143      26
 
NspI r_CATG'y

Cuts at:      0     132     733    1455 
   Size:        132     601     722

  Fragments arranged by size: 

                 722     601     132
 
PasI CC'CwG_GG

Cuts at:      0     747    1455 
   Size:        747     708
 
PfoI T'CCnGG_A

Cuts at:      0     401    1455 
   Size:        401    1054
 
PpuMI rG'GwC_Cy

Cuts at:      0     522     584     810    1455 
   Size:        522      62     226     645

  Fragments arranged by size: 

                 645     522     226      62
 
PspOMI G'GGCC_C

Cuts at:      0    1300    1455 
   Size:       1300     155
 
PstI C_TGCA'G

Cuts at:      0     458    1455 
   Size:        458     997
 
PvuII CAG'CTG

Cuts at:      0     471    1236    1455 
   Size:        471     765     219

  Fragments arranged by size: 

                 765     471     219
 
SacII CC_GC'GG

Cuts at:      0     529    1455 
   Size:        529     926
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40     944    1455 
   Size:         40     904     511

  Fragments arranged by size: 

                 904     511      40
 
SfcI C'TryA_G

Cuts at:      0     454     919    1455 
   Size:        454     465     536

  Fragments arranged by size: 

                 536     465     454
 
SfoI GGC'GCC

Cuts at:      0     187    1455 
   Size:        187    1268
 
SmaI CCC'GGG

Cuts at:      0     196    1251    1455 
   Size:        196    1055     204

  Fragments arranged by size: 

                1055     204     196
 
StyI C'CwwG_G

Cuts at:      0    1217    1455 
   Size:       1217     238
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     670    1455 
   Size:        670     785
 
TatI w'GTAC_w

Cuts at:      0     720     993    1455 
   Size:        720     273     462

  Fragments arranged by size: 

                 720     462     273
 
Tth111I GACn'n_nGTC

Cuts at:      0     443    1403    1455 
   Size:        443     960      52

  Fragments arranged by size: 

                 960     443      52
 
XbaI T'CTAG_A

Cuts at:      0    1308    1455 
   Size:       1308     147
 
XmaI C'CCGG_G

Cuts at:      0     194    1249    1455 
   Size:        194    1055     206

  Fragments arranged by size: 

                1055     206     194
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BlpI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI    BsrGI
   BssHII   BstAPI    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRV    HaeII    Hin4I   HincII     KasI     MmeI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PasI     PfoI    PpuMI   PspOMI
     PstI    PvuII    SacII     SapI     SfcI     SfoI     SmaI     StyI
    TaqII     TatI  Tth111I     XbaI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BclI    BfrBI     BglI    BglII     BmrI     BmtI
    BpuEI    BsaAI    BsaBI    BsaWI    BseRI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrDI    BssSI    BstBI   BstEII    BstYI  BstZ17I
     DraI     DrdI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI     XcmI     XhoI     XmnI     ZraI
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