################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI CelII ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI HphI KasI KpnI KspI MaeII MluI MroI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 521 CGCG CG^CG AlwNI 957 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 472 CCTAAGG CC^T[ACGT]AGG Asp700I 202 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 665 TGGCCA TGG^CCA BamHI 577 GGATCC G^GATCC BanI 853 GGCACC G^G[CT][GA]CC BbsI 821 GTCTTC GAAGAC(2/6) BcnI 1449 CCCGG CC^[GC]GG BmyI 455 GGGCAC G[AGT]GC[ACT]^C BpuAI 821 GTCTTC GAAGAC(2/6) BsaBI 578 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 305 GGGAC GGGAC(10/14) Bsp1286I 455 GGGCAC G[AGT]GC[ACT]^C BspHI 234 TCATGA T^CATGA BsrDI 1148 GCAATG GCAATG(2/0) BsrFI 485 GCCGGC [GA]^CCGG[CT] Bst1107I 808 GTATAC GTA^TAC BstUI 521 CGCG CG^CG BstXI 566 CCATTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 577 GGATCC [GA]^GATC[CT] BstZ17I 808 GTATAC GTA^TAC Bsu36I 472 CCTAAGG CC^T[ACGT]AGG CfoI 520 GCGC GCG^C Cfr10I 485 GCCGGC [GA]^CCGG[CT] DpnII 578 GATC ^GATC DraI 1155 TTTAAA TTT^AAA EaeI 665 TGGCCA [CT]^GGCC[GA] EarI 1261 GAAGAG CTCTTC(1/4) Eco81I 472 CCTAAGG CC^T[ACGT]AGG EcoRI 1168 GAATTC G^AATTC FauI 545 CCCGC CCCGC(4/6) HhaI 520 GCGC GCG^C HinP1I 520 GCGC G^CGC HindIII 660 AAGCTT A^AGCTT Ksp632I 1261 GAAGAG CTCTTC(1/4) MamI 578 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 578 GATC ^GATC MfeI 1319 CAATTG C^AATTG MflI 577 GGATCC [GA]^GATC[CT] MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA MspA1I 830 CAGCTG C[AC]G^C[GT]G MunI 1319 CAATTG C^AATTG MvnI 521 CGCG CG^CG NaeI 485 GCCGGC GCC^GGC NciI 1449 CCCGG CC^[GC]GG NdeI 713 CATATG CA^TATG NdeII 578 GATC ^GATC NgoMIV 485 GCCGGC G^CCGGC PflMI 1404 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 620 GACTC GAGTC(4/5) PvuII 830 CAGCTG CAG^CTG RcaI 234 TCATGA T^CATGA Sau3AI 578 GATC ^GATC SexAI 889 ACCTGGT A^CC[AT]GGT TaqI 141 TCGA T^CGA TthHB8I 141 TCGA T^CGA Van91I 1404 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 577 GGATCC [GA]^GATC[CT] XmnI 202 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 808 GTATAC GT^[AC][GT]AC 1185 GTCTAC AciI 546 CCGC CCGC(-3/-1) 155 GCGG AlwI 578 GATCC GGATC(4/5) 577 GGATC BfaI 719 CTAG C^TAG 805 CTAG BpmI 633 CTGGAG CTGGAG(16/14) 1161 CTGGAG BstNI 890 CCTGG CC^[AT]GG 1119 CCTGG DraII 305 GGGACCT [GA]G^G[ACGT]CC[CT] 468 AGGACCT EcoO109I 305 GGGACCT [GA]G^G[ACGT]CC[CT] 468 AGGACCT EcoRII 890 CCTGG ^CC[AT]GG 1119 CCTGG EcoT14I 877 CCTTGG C^C[AT][AT]GG 1101 CCTTGG HaeIII 641 GGCC GG^CC 666 GGCC HapII 486 CCGG C^CGG 1450 CCGG HpaII 486 CCGG C^CGG 1450 CCGG MaeI 719 CTAG C^TAG 805 CTAG MspI 486 CCGG C^CGG 1450 CCGG MvaI 890 CCTGG CC^[AT]GG 1119 CCTGG MwoI 26 GCCCTAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1091 GCATAATGAGC PpuMI 305 GGGACCT [GA]G^G[AT]CC[CT] 468 AGGACCT ScaI 1216 AGTACT AGT^ACT 1265 AGTACT StyI 877 CCTTGG C^C[AT][AT]GG 1101 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI ApoI AvaII BbvI BsaJI BsiYI BslI BsmAI BspMI BsrI BssKI BstF5I Cac8I Cfr13I DdeI Fnu4HI FokI HinfI ItaI MaeIII MboII MnlI MseI MslI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI