Restriction map for pF1KB7402
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AviII       AvrII       BamHI       BbeI        
BbsI        BbvCI       BbvI        BcnI        BglI        
BglII       BlnI        BpuAI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        Fnu4HI      FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        ItaI        
KasI        Ksp632I     KspI        MfeI        MluI        
MroI        MspA1I      MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TseI        Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        465  GGTACC               G^GTACC
AccII         601  CGCG                 CG^CG
AcsI          840  GAATTT               [GA]^AATT[CT]
AflII         214  CTTAAG               C^TTAAG
AlwNI         514  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          723  GGGCCC               GGGCC^C
ApoI          840  GAATTT               [GA]^AATT[CT]
Asp700I       195  GAAATCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       465  GGTACC               G^GTACC
AspHI         350  GAGCAC               G[AT]GC[AT]^C
AspI          152  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           22  CCCGAG               C^[CT]CG[GA]G
BbrPI         117  CACGTG               CAC^GTG
BcgI          632  CGACCCAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         778  GTATCC               GTATCC(6/5)
BclI          233  TGATCA               T^GATCA
BfaI          992  CTAG                 C^TAG
BfrI          214  CTTAAG               C^TTAAG
BlpI          642  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      642  GCTTAGC              GC^T[ACGT]AGC
BsaAI         117  CACGTG               [CT]AC^GT[GA]
BsaBI         678  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          157  GGTCTC               GGTCTC(1/5)
BsaWI         527  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       350  GAGCAC               G[AT]GC[AT]^C
BsoBI          22  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      269  TGTACA               T^GTACA
BspHI         734  TCATGA               T^CATGA
BspMI         246  GCAGGT               ACCTGC(4/8)
BsrDI        1060  GCAATG               GCAATG(2/0)
BsrGI         269  TGTACA               T^GTACA
BstUI         601  CGCG                 CG^CG
CelII         642  GCTTAGC              GC^T[ACGT]AGC
DsaI          867  CCGTGG               C^C[GA][CT]GG
FbaI          233  TGATCA               T^GATCA
HapII         528  CCGG                 C^CGG
HinfI         106  GATTC                G^A[ACGT]TC
HpaII         528  CCGG                 C^CGG
KpnI          465  GGTACC               GGTAC^C
MaeI          992  CTAG                 C^TAG
MaeIII        662  GTGAC                ^GT[ACGT]AC
MamI          678  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         837  GAAGA                GAAGA(8/7)
MspI          528  CCGG                 C^CGG
MvnI          601  CGCG                 CG^CG
PflFI         152  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         117  CACGTG               CAC^GTG
PmlI          117  CACGTG               CAC^GTG
RcaI          734  TCATGA               T^CATGA
SfcI          393  CTATAG               C^T[GA][CT]AG
SspBI         269  TGTACA               T^GTACA
TfiI          106  GATTC                G^A[AT]TC
Tsp45I        662  GTGAC                ^GT[GC]AC
Tth111I       152  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          195  GAAATCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          270  GTAC                 GT^AC
              466  GTAC                
BalI          668  TGGCCA               TGG^CCA
              848  TGGCCA              
BanII         723  GGGCCC               G[GA]GC[CT]^C
              863  GAGCCC              
BseRI         225  CTCCTC               GAGGAG(10/8)
              769  GAGGAG              
BsgI          330  GTGCAG               GTGCAG(16/14)
              828  GTGCAG              
BstYI         254  AGATCC               [GA]^GATC[CT]
              377  AGATCC              
DraII         722  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              723  GGGCCCT             
DraIII        114  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1076  CACTTAGTG           
EaeI          668  TGGCCA               [CT]^GGCC[GA]
              848  TGGCCA              
EcoNI         714  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              807  CCTGAATGAGG         
EcoO109I      722  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              723  GGGCCCT             
HgaI          337  GCGTC                GACGC(5/10)
              602  GCGTC               
HphI         1044  GGTGA                GGTGA(8/7)
              113  TCACC               
MaeII         118  ACGT                 A^CGT
              388  ACGT                
MflI          254  AGATCC               [GA]^GATC[CT]
              377  AGATCC              
MluNI         668  TGGCCA               TGG^CCA
              848  TGGCCA              
MscI          668  TGGCCA               TGG^CCA
              848  TGGCCA              
MseI          215  TTAA                 T^TAA
             1013  TTAA                
MslI           87  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              510  CACTCAGATG          
RsaI          270  GTAC                 GT^AC
              466  GTAC                
SmlI          142  CTCAAG               C^T[CT][GA]AG
              214  CTTAAG              
TaqI          686  TCGA                 T^CGA
              857  TCGA                
Tru9I         215  TTAA                 T^TAA
             1013  TTAA                
Tsp509I       274  AATT                 ^AATT
              841  AATT                
TthHB8I       686  TCGA                 T^CGA
              857  TCGA                
XhoII         254  AGATCC               [GA]^GATC[CT]
              377  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      EcoT14I     FokI        HaeIII      MboI        
MnlI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TspRI       XcmI        
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