Restriction map for
pF1KB7401
(Circular) (Six-base) MAPSORT of: pF1KB7401.seq Check: 7385 from: 1 to: 3704
pF1KB7401 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 1529 1961 2708 2976 309
Size: 1220 432 747 268 1037
Fragments arranged by size:
1220 1037 747 432 268
AfeI AGC'GCT
Cuts at: 283 283
Size: 3704
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AleI CACnn'nnGTG
Cuts at: 487 551 487
Size: 64 3640
AlwNI CAG_nnn'CTG
Cuts at: 1009 2576 1009
Size: 1567 2137
ApaI G_GGCC'C
Cuts at: 307 307
Size: 3704
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 724 724
Size: 3704
BamHI G'GATC_C
Cuts at: 729 729
Size: 3704
BanI G'GyrC_C
Cuts at: 535 720 1134 1382 1417 535
Size: 185 414 248 35 2822
Fragments arranged by size:
2822 414 248 185 35
BanII G_rGCy'C
Cuts at: 307 415 563 640 718 1748 307
Size: 108 148 77 78 1030 2263
Fragments arranged by size:
2263 1030 148 108 78 77
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BbsI GAAGACnn'nnnn_
Cuts at: 453 462 453
Size: 9 3695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 598 632 598
Size: 34 3670
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 250 816 250
Size: 566 3138
Bme1580I G_kGCm'C
Cuts at: 307 538 1329 1422 2478 307
Size: 231 791 93 1056 1533
Fragments arranged by size:
1533 1056 791 231 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3408 3408
Size: 3704
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 1135 1383 2085 3581 1135
Size: 248 702 1496 1258
Fragments arranged by size:
1496 1258 702 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 427 397
Size: 30 3674
BseYI C'CCAG_C
Cuts at: 582 666 1129 1887 2464 2960 582
Size: 84 463 758 577 496 1326
Fragments arranged by size:
1326 758 577 496 463 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 313 289
Size: 24 3680
BsiEI CG_ry'CG
Cuts at: 104 219 314 1246 1292 2500 104
Size: 115 95 932 46 1208 1308
Fragments arranged by size:
1308 1208 932 115 95 46
BsiHKAI G_wGCw'C
Cuts at: 563 718 1496 1686 2478 563
Size: 155 778 190 792 1789
Fragments arranged by size:
1789 792 778 190 155
Bsp1286I G_dGCh'C
Cuts at: 307 415 538 563 640 718 1329 1422 1496
Size: 108 123 25 77 78 611 93 74
Cuts at: 1496 1686 1748 2478 307
Size: 190 62 730 1533
Fragments arranged by size:
1533 730 611 190 123 108 93 78
77 74 62 25
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 473 668 1616 473
Size: 195 948 2561
Fragments arranged by size:
2561 948 195
BsrFI r'CCGG_y
Cuts at: 309 1702 1883 2092 309
Size: 1393 181 209 1921
Fragments arranged by size:
1921 1393 209 181
BssSI C'ACGA_G
Cuts at: 406 1975 2333 406
Size: 1569 358 1777
Fragments arranged by size:
1777 1569 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstYI r'GATC_y
Cuts at: 1 729 1196 1219 1554 1800 2812 2898 2910
Size: 728 467 23 335 246 1012 86 12
Cuts at: 2910 3256 1
Size: 346 449
Fragments arranged by size:
1012 728 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 213 107
Size: 106 3598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 947 947
Size: 3704
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DraIII CAC_nnn'GTG
Cuts at: 509 509
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 201 216 311 359 1289 1463 1854 1881 3226
Size: 15 95 48 930 174 391 27 1345
Cuts at: 3226 201
Size: 679
Fragments arranged by size:
1345 930 679 391 174 95 48 27
15
EagI C'GGCC_G
Cuts at: 216 311 1289 216
Size: 95 978 2631
Fragments arranged by size:
2631 978 95
EarI CTCTTCn'nnn_
Cuts at: 235 391 551 1727 1937 235
Size: 156 160 1176 210 2002
Fragments arranged by size:
2002 1176 210 160 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 414 588 1912 2222 2368 3514 414
Size: 174 1324 310 146 1146 604
Fragments arranged by size:
1324 1146 604 310 174 146
EcoICRI GAG'CTC
Cuts at: 561 716 561
Size: 155 3549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 547 1529 1961 2708 2976 309
Size: 238 982 432 747 268 1037
Fragments arranged by size:
1037 982 747 432 268 238
EcoNI CCTnn'n_nnAGG
Cuts at: 397 397
Size: 3704
EcoO109I rG'GnC_Cy
Cuts at: 303 843 303
Size: 540 3164
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 673 1723 673
Size: 1050 2654
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 115 257 285 452 1138 1386 2408 3387 115
Size: 142 28 167 686 248 1022 979 432
Fragments arranged by size:
1022 979 686 432 248 167 142 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1546 1576 1578 1608 1546
Size: 30 2 30 3642
Fragments arranged by size:
3642 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MfeI C'AATT_G
Cuts at: 620 620
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2559 2375
Size: 184 3520
MscI TGG'CCA
Cuts at: 1465 1465
Size: 3704
MslI CAynn'nnrTG
Cuts at: 487 551 1693 1820 487
Size: 64 1142 127 2371
Fragments arranged by size:
2371 1142 127 64
MspA1I CmG'CkG
Cuts at: 189 215 221 551 815 1129 1489 2502 2747
Size: 26 6 330 264 314 360 1013 245
Cuts at: 2747 189
Size: 1146
Fragments arranged by size:
1146 1013 360 330 314 264 245 26
6
NaeI GCC'GGC
Cuts at: 311 1885 311
Size: 1574 2130
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 107
Size: 3704
NgoMIV G'CCGG_C
Cuts at: 309 1883 309
Size: 1574 2130
NotI GC'GGCC_GC
Cuts at: 216 216
Size: 3704
NspI r_CATG'y
Cuts at: 246 757 1257 2164 246
Size: 511 500 907 1786
Fragments arranged by size:
1786 907 511 500
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PfoI T'CCnGG_A
Cuts at: 563 563
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PspOMI G'GGCC_C
Cuts at: 303 303
Size: 3704
PstI C_TGCA'G
Cuts at: 751 1436 751
Size: 685 3019
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 551 1129 1489 551
Size: 578 360 2766
Fragments arranged by size:
2766 578 360
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 563 718 563
Size: 155 3549
SacII CC_GC'GG
Cuts at: 216 216
Size: 3704
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 235 391 551 1727 1937 235
Size: 156 160 1176 210 2002
Fragments arranged by size:
2002 1176 210 160 156
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 747 1432 2425 2616 33
Size: 714 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 191
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 726 726
Size: 3704
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 107 838 1192 107
Size: 731 354 2619
Fragments arranged by size:
2619 731 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1287 1420 1879 2088 1287
Size: 133 459 209 2903
Fragments arranged by size:
2903 459 209 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 3221 3253 397
Size: 32 2792 32 848
Fragments arranged by size:
2792 848 32 32
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 1199 492
Size: 707 2997
XmaI C'CCGG_G
Cuts at: 724 724
Size: 3704
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI