Restriction map for pF1KB7396
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          360  GTAC                 GT^AC
AflIII         92  ACATGT               A^C[GA][CT]GT
AgeI          205  ACCGGT               A^CCGGT
AlwI          499  GATCC                GGATC(4/5)
AvaII         566  GGTCC                G^G[AT]CC
BbsI          484  GAAGAC               GAAGAC(2/6)
BcnI          530  CCGGG                CC^[GC]GG
BglI          529  GCCGGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          443  CTGGAG               CTGGAG(16/14)
BpuAI         484  GAAGAC               GAAGAC(2/6)
BsaWI         205  ACCGGT               [AT]^CCGG[AT]
BseRI         520  GAGGAG               GAGGAG(10/8)
BspLU11I       92  ACATGT               A^CATGT
BspMI         218  ACCTGC               ACCTGC(4/8)
BsrBI         149  GAGCGG               CCGCTC(-3/-3)
BsrFI         205  ACCGGT               [GA]^CCGG[CT]
Cfr10I        205  ACCGGT               [GA]^CCGG[CT]
DdeI          413  CTCAG                C^T[ACGT]AG
DpnII         499  GATC                 ^GATC
DraII         323  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        109  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          517  GAAGAG               CTCTTC(1/4)
EcoO109I      323  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI          291  GCGGG                CCCGC(4/6)
HaeIII        324  GGCC                 GG^CC
HindIII       556  AAGCTT               A^AGCTT
HphI          196  GGTGA                GGTGA(8/7)
Ksp632I       517  GAAGAG               CTCTTC(1/4)
MboI          499  GATC                 ^GATC
NciI          530  CCGGG                CC^[GC]GG
NdeII         499  GATC                 ^GATC
PflMI         502  CCAGAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         205  ACCGGT               A^CCGGT
RsaI          360  GTAC                 GT^AC
Sau3AI        499  GATC                 ^GATC
SfaNI         526  GATGC                GCATC(5/9)
SgrAI         204  CACCGGTG             C[GA]^CCGG[CT]G
SphI          211  GCATGC               GCATG^C
TaqI          343  TCGA                 T^CGA
TspRI         259  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       343  TCGA                 T^CGA
Van91I        502  CCAGAGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          151  GCGG                 CCGC(-3/-1)
              291  GCGG                
AcsI           75  AAATTT               [GA]^AATT[CT]
              378  AAATTC              
ApoI           75  AAATTT               [GA]^AATT[CT]
              378  AAATTC              
BanI          202  GGCACC               G^G[CT][GA]CC
              263  GGCACC              
BsmBI         141  GAGACG               CGTCTC(1/5)
              446  GAGACG              
BsmFI         293  GGGAC                GGGAC(10/14)
              572  GTCCC               
Cfr13I        324  GGCCC                G^G[ACGT]CC
              566  GGTCC               
HapII         206  CCGG                 C^CGG
              530  CCGG                
HpaII         206  CCGG                 C^CGG
              530  CCGG                
MaeII         144  ACGT                 A^CGT
              389  ACGT                
MspI          206  CCGG                 C^CGG
              530  CCGG                
MwoI          529  GCCGGGCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              534  GCTGGCAGGGC         
NlaIV         202  GGCACC               GG[ACGT]^[ACGT]CC
              263  GGCACC              
NspI           92  ACATGT               [GA]CATG^[CT]
              211  GCATGC              
Sau96I        324  GGCCC                G^G[ACGT]CC
              566  GGTCC               
Tsp509I        76  AATT                 ^AATT
              379  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       EcoRII      Fnu4HI      FokI        ItaI        
MboII       MnlI        MvaI        NlaIII      ScrFI       
TseI        
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