(Circular) (Six-base) MAPSORT of: pF1KB7394.seq Check: 1126 from: 1 to: 3725 pF1KB7394 3725 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 763 763 Size: 3725 Acc65I G'GTAC_C Cuts at: 741 741 Size: 3725 AclI AA'CG_TT Cuts at: 3565 3565 Size: 3725 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 375 1550 1982 2729 2997 375 Size: 1175 432 747 268 1103 Fragments arranged by size: 1175 1103 747 432 268 AfeI AGC'GCT Cuts at: 208 208 Size: 3725 AflIII A'CryG_T Cuts at: 1077 2181 3158 1077 Size: 1104 977 1644 Fragments arranged by size: 1644 1104 977 AgeI A'CCGG_T Cuts at: 2113 2113 Size: 3725 AlwNI CAG_nnn'CTG Cuts at: 1030 2597 1030 Size: 1567 2158 ApaLI G'TGCA_C Cuts at: 2495 2495 Size: 3725 ApoI r'AATT_y Cuts at: 712 729 3078 3334 712 Size: 17 2349 256 1103 Fragments arranged by size: 2349 1103 256 17 AseI AT'TA_AT Cuts at: 20 20 Size: 3725 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3725 AvaI C'yCGr_G Cuts at: 745 745 Size: 3725 BamHI G'GATC_C Cuts at: 750 750 Size: 3725 BanI G'GyrC_C Cuts at: 182 539 557 741 1155 1403 1438 182 Size: 357 18 184 414 248 35 2469 Fragments arranged by size: 2469 414 357 248 184 35 18 BanII G_rGCy'C Cuts at: 166 288 497 739 1769 166 Size: 122 209 242 1030 2122 Fragments arranged by size: 2122 1030 242 209 122 BbeI G_GCGC'C Cuts at: 1159 1407 1159 Size: 248 3477 BbsI GAAGACnn'nnnn_ Cuts at: 457 457 Size: 3725 BciVI GTATCCnnnnn_n' Cuts at: 1623 2384 3725 1623 Size: 761 1341 1623 Fragments arranged by size: 1623 1341 761 BclI T'GATC_A Cuts at: 1245 1245 Size: 3725 BglII A'GATC_T Cuts at: 1240 3277 1240 Size: 2037 1688 BlpI GC'TnA_GC Cuts at: 599 837 599 Size: 238 3487 Bme1580I G_kGCm'C Cuts at: 481 1350 1443 2499 481 Size: 869 93 1056 1707 Fragments arranged by size: 1707 1056 869 93 BmgBI CAC'GTC Cuts at: 627 627 Size: 3725 BmrI ACTGGGnnnn_n' Cuts at: 470 694 937 1018 1353 3497 470 Size: 224 243 81 335 2144 698 Fragments arranged by size: 2144 698 335 243 224 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 3725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 901 1780 2272 2570 901 Size: 879 492 298 2056 Fragments arranged by size: 2056 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 460 3429 460 Size: 2969 756 BsaAI yAC'GTr Cuts at: 1078 1078 Size: 3725 BsaBI GATnn'nnATC Cuts at: 6 1239 3276 6 Size: 1233 2037 455 Fragments arranged by size: 2037 1233 455 BsaHI Gr'CG_yC Cuts at: 1156 1404 2106 3602 1156 Size: 248 702 1496 1279 Fragments arranged by size: 1496 1279 702 248 BsaWI w'CCGG_w Cuts at: 914 1435 2113 2387 2534 2945 914 Size: 521 678 274 147 411 1694 Fragments arranged by size: 1694 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 362 362 Size: 3725 BseYI C'CCAG_C Cuts at: 352 382 586 1150 1908 2485 2981 352 Size: 30 204 564 758 577 496 1096 Fragments arranged by size: 1096 758 577 564 496 204 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 348 348 Size: 3725 BsiEI CG_ry'CG Cuts at: 104 547 1267 1313 2521 104 Size: 443 720 46 1208 1308 Fragments arranged by size: 1308 1208 720 443 46 BsiHKAI G_wGCw'C Cuts at: 166 248 739 1517 1707 2499 166 Size: 82 491 778 190 792 1392 Fragments arranged by size: 1392 792 778 491 190 82 Bsp1286I G_dGCh'C Cuts at: 166 248 288 481 497 739 1350 1443 1517 Size: 82 40 193 16 242 611 93 74 Cuts at: 1517 1707 1769 2499 166 Size: 190 62 730 1392 Fragments arranged by size: 1392 730 611 242 193 190 93 82 74 62 40 16 BspEI T'CCGG_A Cuts at: 2945 2945 Size: 3725 BspHI T'CATG_A Cuts at: 2901 2901 Size: 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 775 989 1291 1672 775 Size: 214 302 381 2828 Fragments arranged by size: 2828 381 302 214 BsrBI CCG'CTC Cuts at: 50 2017 2071 3555 3720 50 Size: 1967 54 1484 165 55 Fragments arranged by size: 1967 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1637 1637 Size: 3725 BsrFI r'CCGG_y Cuts at: 1723 1904 2113 1723 Size: 181 209 3335 Fragments arranged by size: 3335 209 181 BsrGI T'GTAC_A Cuts at: 335 335 Size: 3725 BssSI C'ACGA_G Cuts at: 1996 2354 1996 Size: 358 3367 BstAPI GCAn_nnn'nTGC Cuts at: 1102 1102 Size: 3725 BstBI TT'CG_AA Cuts at: 2086 2086 Size: 3725 BstYI r'GATC_y Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931 Size: 749 467 23 335 246 1012 86 12 Cuts at: 2931 3277 1 Size: 346 449 Fragments arranged by size: 1012 749 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 689 1675 1856 2019 689 Size: 986 181 163 2395 Fragments arranged by size: 2395 986 181 163 BtsI GCAGTG_nn' Cuts at: 968 968 Size: 3725 DraI TTT'AAA Cuts at: 724 724 Size: 3725 DrdI GACnn_nn'nnGTC Cuts at: 1431 2289 1431 Size: 858 2867 EaeI y'GGCC_r Cuts at: 1310 1484 1875 1902 3247 1310 Size: 174 391 27 1345 1788 Fragments arranged by size: 1788 1345 391 174 27 EagI C'GGCC_G Cuts at: 1310 1310 Size: 3725 EarI CTCTTCn'nnn_ Cuts at: 1748 1958 1748 Size: 210 3515 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1933 2243 2389 3535 1933 Size: 310 146 1146 2123 Fragments arranged by size: 2123 1146 310 146 EcoICRI GAG'CTC Cuts at: 164 737 164 Size: 573 3152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 375 1550 1982 2729 2997 375 Size: 1175 432 747 268 1103 Fragments arranged by size: 1175 1103 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 864 864 Size: 3725 EcoRI G'AATT_C Cuts at: 729 729 Size: 3725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1744 1744 Size: 3725 FspI TGC'GCA Cuts at: 1506 1506 Size: 3725 HaeII r_GCGC'y Cuts at: 210 1159 1407 2429 3408 210 Size: 949 248 1022 979 527 Fragments arranged by size: 1022 979 949 527 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1567 1597 1599 1629 1567 Size: 30 2 30 3663 Fragments arranged by size: 3663 30 30 2 HincII GTy'rAC Cuts at: 764 764 Size: 3725 KasI G'GCGC_C Cuts at: 1155 1403 1155 Size: 248 3477 KpnI G_GTAC'C Cuts at: 745 745 Size: 3725 MluI A'CGCG_T Cuts at: 3158 3158 Size: 3725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2396 2580 2396 Size: 184 3541 MscI TGG'CCA Cuts at: 1486 1486 Size: 3725 MslI CAynn'nnrTG Cuts at: 1714 1841 1714 Size: 127 3598 MspA1I CmG'CkG Cuts at: 836 1150 1510 2523 2768 836 Size: 314 360 1013 245 1793 Fragments arranged by size: 1793 1013 360 314 245 NaeI GCC'GGC Cuts at: 1906 1906 Size: 3725 NarI GG'CG_CC Cuts at: 1156 1404 1156 Size: 248 3477 NdeI CA'TA_TG Cuts at: 446 446 Size: 3725 NgoMIV G'CCGG_C Cuts at: 1904 1904 Size: 3725 NspI r_CATG'y Cuts at: 241 778 1278 2185 241 Size: 537 500 907 1781 Fragments arranged by size: 1781 907 537 500 PasI CC'CwG_GG Cuts at: 168 299 168 Size: 131 3594 PciI A'CATG_T Cuts at: 2181 2181 Size: 3725 PflMI CCAn_nnn'nTGG Cuts at: 113 280 412 113 Size: 167 132 3426 Fragments arranged by size: 3426 167 132 PmeI GTTT'AAAC Cuts at: 724 724 Size: 3725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2864 2896 2864 Size: 32 3693 PstI C_TGCA'G Cuts at: 772 1457 772 Size: 685 3040 PvuI CG_AT'CG Cuts at: 104 104 Size: 3725 PvuII CAG'CTG Cuts at: 1150 1510 1150 Size: 360 3365 RsrII CG'GwC_CG Cuts at: 1920 1920 Size: 3725 SacI G_AGCT'C Cuts at: 166 739 166 Size: 573 3152 SalI G'TCGA_C Cuts at: 762 762 Size: 3725 SapI GCTCTTCn'nnn_ Cuts at: 1748 1958 1748 Size: 210 3515 SbfI CC_TGCA'GG Cuts at: 772 772 Size: 3725 ScaI AGT'ACT Cuts at: 3242 3242 Size: 3725 SfcI C'TryA_G Cuts at: 33 768 1453 2446 2637 33 Size: 735 685 993 191 1121 Fragments arranged by size: 1121 993 735 685 191 SfoI GGC'GCC Cuts at: 1157 1405 1157 Size: 248 3477 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3725 SmaI CCC'GGG Cuts at: 747 747 Size: 3725 SmlI C'TyrA_G Cuts at: 880 1795 2287 2549 880 Size: 915 492 262 2056 Fragments arranged by size: 2056 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3725 SphI G_CATG'C Cuts at: 241 778 1278 241 Size: 537 500 2688 Fragments arranged by size: 2688 537 500 StyI C'CwwG_G Cuts at: 406 423 859 1213 406 Size: 17 436 354 2918 Fragments arranged by size: 2918 436 354 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1308 1441 1900 2109 1308 Size: 133 459 209 2924 Fragments arranged by size: 2924 459 209 133 TatI w'GTAC_w Cuts at: 335 3240 335 Size: 2905 820 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3242 3274 3242 Size: 32 3693 Tth111I GACn'n_nGTC Cuts at: 548 1522 548 Size: 974 2751 XbaI T'CTAG_A Cuts at: 756 756 Size: 3725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1220 1220 Size: 3725 XmaI C'CCGG_G Cuts at: 745 745 Size: 3725 XmnI GAAnn'nnTTC Cuts at: 454 454 Size: 3725 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI