Restriction map for pF1KB7393
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BcgI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CelII       ClaI        DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HaeII       HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MluI        MluNI       MroI        
MscI        MspA1I      NarI        NcoI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII         166  CGCG                 CG^CG
AcyI           73  GGCGTC               G[GA]^CG[CT]C
AfaI         1172  GTAC                 GT^AC
AflII         751  CTTAAG               C^TTAAG
AlwI          897  GGATC                GGATC(4/5)
AocI          534  CCTTAGG              CC^T[ACGT]AGG
BbsI          378  GAAGAC               GAAGAC(2/6)
BbvCI         696  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1053  GGATAC               GTATCC(6/5)
BclI           26  TGATCA               T^GATCA
BcnI           63  CCGGG                CC^[GC]GG
BfrI          751  CTTAAG               C^TTAAG
BpuAI         378  GAAGAC               GAAGAC(2/6)
BsaHI          73  GGCGTC               G[GA]^CG[CT]C
BsaI          590  GAGACC               GGTCTC(1/5)
BsmFI         169  GGGAC                GGGAC(10/14)
BsmI         1128  GCATTC               GAATGC(1/-1)
BstF5I        422  CATCC                GGATG(2/0)
BstUI         166  CGCG                 CG^CG
BstYI         897  GGATCT               [GA]^GATC[CT]
Bsu36I        534  CCTTAGG              CC^T[ACGT]AGG
CfoI          165  GCGC                 GCG^C
CpoI          125  CGGTCCG              CG^G[AT]CCG
DraII         169  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1141  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        534  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      169  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         265  GATATC               GAT^ATC
FbaI           26  TGATCA               T^GATCA
FokI          422  CATCC                GGATG(9/13)
HaeIII        282  GGCC                 GG^CC
HgaI           74  GCGTC                GACGC(5/10)
HhaI          165  GCGC                 GCG^C
Hin1I          73  GGCGTC               G[GA]^CG[CT]C
HinP1I        165  GCGC                 G^CGC
MfeI          584  CAATTG               C^AATTG
MflI          897  GGATCT               [GA]^GATC[CT]
MslI          369  CACACCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          584  CAATTG               C^AATTG
MvnI          166  CGCG                 CG^CG
NaeI           32  GCCGGC               GCC^GGC
NciI           63  CCGGG                CC^[GC]GG
NdeI         1010  CATATG               CA^TATG
NgoMIV         32  GCCGGC               G^CCGGC
NlaIII       1119  CATG                 CATG^
PpuMI         169  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         547  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI         1172  GTAC                 GT^AC
RsrII         125  CGGTCCG              CG^G[AT]CCG
TaqI          840  TCGA                 T^CGA
TthHB8I       840  TCGA                 T^CGA
XhoII         897  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

BbvI          835  GCAGC                GCAGC(8/12)
              948  GCTGC               
BfaI          863  CTAG                 C^TAG
             1207  CTAG                
BpmI          670  CTCCAG               CTGGAG(16/14)
              742  CTCCAG              
BsgI          980  CTGCAC               GTGCAG(16/14)
              727  GTGCAG              
BsmAI         321  GAGAC                GTCTC(1/5)
              590  GAGAC               
BsrFI          32  GCCGGC               [GA]^CCGG[CT]
              123  GCCGGT              
BstNI         110  CCAGG                CC^[AT]GG
              744  CCAGG               
Cfr10I         32  GCCGGC               [GA]^CCGG[CT]
              123  GCCGGT              
DpnII          27  GATC                 ^GATC
              898  GATC                
EcoRII        110  CCAGG                ^CC[AT]GG
              744  CCAGG               
EcoT14I        47  CCTTGG               C^C[AT][AT]GG
              464  CCTTGG              
HindIII       571  AAGCTT               A^AGCTT
              847  AAGCTT              
HphI           24  GGTGA                GGTGA(8/7)
              209  TCACC               
MaeI          863  CTAG                 C^TAG
             1207  CTAG                
MaeIII        554  GTCAC                ^GT[ACGT]AC
              678  GTCAC               
MboI           27  GATC                 ^GATC
              898  GATC                
MvaI          110  CCAGG                CC^[AT]GG
              744  CCAGG               
MwoI          167  GCGGGACCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              524  GCTTAAAAAGC         
NdeII          27  GATC                 ^GATC
              898  GATC                
PflMI        1044  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1140  CCACACTGTGG         
Sau3AI         27  GATC                 ^GATC
              898  GATC                
SmlI          549  CTTGAG               C^T[CT][GA]AG
              751  CTTAAG              
StyI           47  CCTTGG               C^C[AT][AT]GG
              464  CCTTGG              
TseI          835  GCAGC                G^C[AT]GC
              948  GCTGC               
Tsp45I        554  GTCAC                ^GT[GC]AC
              678  GTCAC               
Van91I       1044  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1140  CCACACTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
AvaII       BsaJI       BsiYI       BslI        BsrI        
BssKI       Cac8I       Cfr13I      DdeI        DraI        
FauI        Fnu4HI      HapII       HinfI       HpaII       
ItaI        MboII       MnlI        MseI        MspI        
NlaIV       PleI        Sau96I      ScrFI       TfiI        
Tru9I       Tsp509I     TspRI       XmnI        
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