Restriction map for pF1KB7389
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BamHI       BbeI        BbsI        BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlpI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          487  GTAGAC               GT^[AC][GT]AC
AccII         181  CGCG                 CG^CG
AlwI           60  GATCC                GGATC(4/5)
AlwNI         430  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII         609  CCTAGG               C^CTAGG
BanI          409  GGTGCC               G^G[CT][GA]CC
BbrPI         570  CACGTG               CAC^GTG
BcgI          468  CGACCACGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          222  CCGGG                CC^[GC]GG
BfaI          610  CTAG                 C^TAG
BlnI          609  CCTAGG               C^CTAGG
BsaAI         570  CACGTG               [CT]AC^GT[GA]
BsaBI         333  GATACTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          506  GTGCAG               GTGCAG(16/14)
BstEII        217  GGTAACC              G^GT[ACGT]ACC
BstPI         217  GGTAACC              G^GT[ACGT]ACC
BstUI         181  CGCG                 CG^CG
BstXI         281  CCATGATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          59  AGATCC               [GA]^GATC[CT]
DraI          135  TTTAAA               TTT^AAA
EaeI          255  TGGCCG               [CT]^GGCC[GA]
EcoO65I       217  GGTAACC              G^GT[ACGT]ACC
EcoT14I       609  CCTAGG               C^C[AT][AT]GG
HapII         222  CCGG                 C^CGG
HpaII         222  CCGG                 C^CGG
HphI          122  TCACC                GGTGA(8/7)
MaeI          610  CTAG                 C^TAG
MamI          333  GATACTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           59  AGATCC               [GA]^GATC[CT]
MslI          282  CATGATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        517  CAGCTG               C[AC]G^C[GT]G
MspI          222  CCGG                 C^CGG
MvnI          181  CGCG                 CG^CG
NciI          222  CCGGG                CC^[GC]GG
NspI           37  ACATGC               [GA]CATG^[CT]
PmaCI         570  CACGTG               CAC^GTG
PmlI          570  CACGTG               CAC^GTG
PvuII         517  CAGCTG               CAG^CTG
SacI          244  GAGCTC               GAGCT^C
SapI          246  GCTCTTC              GCTCTTC(1/4)
SfaNI          96  GATGC                GCATC(5/9)
SfcI          105  CTACAG               C^T[GA][CT]AG
StyI          609  CCTAGG               C^C[AT][AT]GG
Tsp45I         34  GTGAC                ^GT[GC]AC
Tsp509I        84  AATT                 ^AATT
XhoII          59  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          213  GTAC                 GT^AC
              300  GTAC                
AspHI         244  GAGCTC               G[AT]GC[AT]^C
              567  GAGCAC              
BanII         244  GAGCTC               G[GA]GC[CT]^C
              402  GAGCCC              
BpmI          241  CTGGAG               CTGGAG(16/14)
              553  CTGGAG              
BsiHKAI       244  GAGCTC               G[AT]GC[AT]^C
              567  GAGCAC              
DraII         224  GGGACCT              [GA]G^G[ACGT]CC[CT]
              595  GGGACCT             
EarI          247  CTCTTC               CTCTTC(1/4)
              386  GAAGAG              
EcoO109I      224  GGGACCT              [GA]G^G[ACGT]CC[CT]
              595  GGGACCT             
HinfI         153  GACTC                G^A[ACGT]TC
              513  GACTC               
Ksp632I       247  CTCTTC               CTCTTC(1/4)
              386  GAAGAG              
MaeII         112  ACGT                 A^CGT
              571  ACGT                
MaeIII         34  GTGAC                ^GT[ACGT]AC
              218  GTAAC               
MseI          136  TTAA                 T^TAA
              344  TTAA                
PleI          153  GACTC                GAGTC(4/5)
              513  GACTC               
PpuMI         224  GGGACCT              [GA]G^G[AT]CC[CT]
              595  GGGACCT             
RsaI          213  GTAC                 GT^AC
              300  GTAC                
Tru9I         136  TTAA                 T^TAA
              344  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      ItaI        MboI        MboII       
MnlI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
TspRI       
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