Restriction map for pF1KB7389
 (Linear) (Six-base) MAPSORT of: KIBB7389.seq  Check: 8302  from: 1  to: 615



KIBB7389.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     488     615 
   Size:        488     127
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     615 
   Size:        541      74
 
AlwNI CAG_nnn'CTG

Cuts at:      0     435     615 
   Size:        435     180
 
AvrII C'CTAG_G

Cuts at:      0     609     615 
   Size:        609       6
 
BanI G'GyrC_C

Cuts at:      0     409     615 
   Size:        409     206
 
BanII G_rGCy'C

Cuts at:      0     248     406     615 
   Size:        248     158     209

  Fragments arranged by size: 

                 248     209     158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     457     491     615 
   Size:        457      34     124

  Fragments arranged by size: 

                 457     124      34
 
Bme1580I G_kGCm'C

Cuts at:      0     414     615 
   Size:        414     201
 
BmgBI CAC'GTC

Cuts at:      0     113     615 
   Size:        113     502
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     537     615 
   Size:        537      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     574     615 
   Size:        262     312      41

  Fragments arranged by size: 

                 312     262      41
 
BsaAI yAC'GTr

Cuts at:      0     572     615 
   Size:        572      43
 
BsaBI GATnn'nnATC

Cuts at:      0     337     615 
   Size:        337     278
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     615 
   Size:        527      88
 
BsiHKAI G_wGCw'C

Cuts at:      0     248     571     615 
   Size:        248     323      44

  Fragments arranged by size: 

                 323     248      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     406     414     571     615 
   Size:        248     158       8     157      44

  Fragments arranged by size: 

                 248     158     157      44       8
 
BstEII G'GTnAC_C

Cuts at:      0     217     615 
   Size:        217     398
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     615 
   Size:        288     327
 
BstYI r'GATC_y

Cuts at:      0      59     615 
   Size:         59     556
 
BtsI GCAGTG_nn'

Cuts at:      0     317     615 
   Size:        317     298
 
DraI TTT'AAA

Cuts at:      0     137     615 
   Size:        137     478
 
EaeI y'GGCC_r

Cuts at:      0     255     615 
   Size:        255     360
 
EarI CTCTTCn'nnn_

Cuts at:      0     253     381     615 
   Size:        253     128     234

  Fragments arranged by size: 

                 253     234     128
 
EcoICRI GAG'CTC

Cuts at:      0     246     615 
   Size:        246     369
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     541     574     615 
   Size:        262     279      33      41

  Fragments arranged by size: 

                 279     262      41      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     225     596     615 
   Size:        225     371      19

  Fragments arranged by size: 

                 371     225      19
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     130     615 
   Size:         73      57     485

  Fragments arranged by size: 

                 485      73      57
 
MslI CAynn'nnrTG

Cuts at:      0     286     615 
   Size:        286     329
 
MspA1I CmG'CkG

Cuts at:      0     519     615 
   Size:        519      96
 
NspI r_CATG'y

Cuts at:      0      41     615 
   Size:         41     574
 
PmlI CAC'GTG

Cuts at:      0     572     615 
   Size:        572      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     225     596     615 
   Size:        225     371      19

  Fragments arranged by size: 

                 371     225      19
 
PvuII CAG'CTG

Cuts at:      0     519     615 
   Size:        519      96
 
SacI G_AGCT'C

Cuts at:      0     248     615 
   Size:        248     367
 
SapI GCTCTTCn'nnn_

Cuts at:      0     253     615 
   Size:        253     362
 
SfcI C'TryA_G

Cuts at:      0     105     615 
   Size:        105     510
 
StyI C'CwwG_G

Cuts at:      0     609     615 
   Size:        609       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      77     370     615 
   Size:         77     293     245

  Fragments arranged by size: 

                 293     245      77
 
TatI w'GTAC_w

Cuts at:      0     299     615 
   Size:        299     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      73     105     615 
   Size:         73      32     510

  Fragments arranged by size: 

                 510      73      32
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI    AvrII     BanI    BanII     BcgI Bme1580I
    BmgBI     BmrI     BpmI    BsaAI    BsaBI     BsgI  BsiHKAI Bsp1286I
   BstEII    BstXI    BstYI     BtsI     DraI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I     MmeI     MslI   MspA1I     NspI     PmlI    PpuMI
    PvuII     SacI     SapI     SfcI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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