Restriction map for pF1KB7386
 (Linear) (Six-base) MAPSORT of: KIBB7386.seq  Check: 735  from: 1  to: 1254



KIBB7386.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      81    1254 
   Size:         81    1173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     524     635    1254 
   Size:        524     111     619

  Fragments arranged by size: 

                 619     524     111
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300     658    1033    1254 
   Size:        300     358     375     221

  Fragments arranged by size: 

                 375     358     300     221
 
ApaLI G'TGCA_C

Cuts at:      0    1215    1254 
   Size:       1215      39
 
ApoI r'AATT_y

Cuts at:      0     133     493     606     793    1254 
   Size:        133     360     113     187     461

  Fragments arranged by size: 

                 461     360     187     133     113
 
BamHI G'GATC_C

Cuts at:      0     625    1254 
   Size:        625     629
 
BanI G'GyrC_C

Cuts at:      0    1185    1254 
   Size:       1185      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      20     914    1254 
   Size:         20     894     340

  Fragments arranged by size: 

                 894     340      20
 
Bme1580I G_kGCm'C

Cuts at:      0    1219    1254 
   Size:       1219      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    1254 
   Size:        410     844
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     674    1254 
   Size:        300     374     580

  Fragments arranged by size: 

                 580     374     300
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     939     942    1254 
   Size:        939       3     312

  Fragments arranged by size: 

                 939     312       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     683    1254 
   Size:        115     568     571

  Fragments arranged by size: 

                 571     568     115
 
BsiHKAI G_wGCw'C

Cuts at:      0    1219    1254 
   Size:       1219      35
 
BsmI GAATG_Cn'

Cuts at:      0     105     150     867    1254 
   Size:        105      45     717     387

  Fragments arranged by size: 

                 717     387     105      45
 
Bsp1286I G_dGCh'C

Cuts at:      0    1219    1254 
   Size:       1219      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      81      95    1254 
   Size:         81      14    1159

  Fragments arranged by size: 

                1159      81      14
 
BsrDI GCAATG_nn'

Cuts at:      0     110    1232    1254 
   Size:        110    1122      22

  Fragments arranged by size: 

                1122     110      22
 
BsrGI T'GTAC_A

Cuts at:      0     993    1254 
   Size:        993     261
 
BstYI r'GATC_y

Cuts at:      0      48     625    1254 
   Size:         48     577     629

  Fragments arranged by size: 

                 629     577      48
 
BtgI C'CryG_G

Cuts at:      0     677    1254 
   Size:        677     577
 
BtsI GCAGTG_nn'

Cuts at:      0    1217    1254 
   Size:       1217      37
 
DraI TTT'AAA

Cuts at:      0     312     498     743    1254 
   Size:        312     186     245     511

  Fragments arranged by size: 

                 511     312     245     186
 
DraIII CAC_nnn'GTG

Cuts at:      0     892    1254 
   Size:        892     362
 
EaeI y'GGCC_r

Cuts at:      0     803    1254 
   Size:        803     451
 
EarI CTCTTCn'nnn_

Cuts at:      0     170     515     588     960    1225    1230    1254 
   Size:        170     345      73     372     265       5      24

  Fragments arranged by size: 

                 372     345     265     170      73      24       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     524     635    1254 
   Size:        410     114     111     619

  Fragments arranged by size: 

                 619     410     114     111
 
EcoRI G'AATT_C

Cuts at:      0     133     606    1254 
   Size:        133     473     648

  Fragments arranged by size: 

                 648     473     133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     349     381     459     491    1254 
   Size:        349      32      78      32     763

  Fragments arranged by size: 

                 763     349      78      32      32
 
HindIII A'AGCT_T

Cuts at:      0     212    1254 
   Size:        212    1042
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1152    1254 
   Size:        475     677     102

  Fragments arranged by size: 

                 677     475     102
 
MscI TGG'CCA

Cuts at:      0     805    1254 
   Size:        805     449
 
MslI CAynn'nnrTG

Cuts at:      0      70    1254 
   Size:         70    1184
 
MspA1I CmG'CkG

Cuts at:      0     685    1254 
   Size:        685     569
 
NcoI C'CATG_G

Cuts at:      0     677    1254 
   Size:        677     577
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     621    1254 
   Size:        621     633
 
PstI C_TGCA'G

Cuts at:      0      92    1254 
   Size:         92    1162
 
PvuII CAG'CTG

Cuts at:      0     685    1254 
   Size:        685     569
 
SapI GCTCTTCn'nnn_

Cuts at:      0     170     515    1254 
   Size:        170     345     739

  Fragments arranged by size: 

                 739     345     170
 
SbfI CC_TGCA'GG

Cuts at:      0      92    1254 
   Size:         92    1162
 
SfcI C'TryA_G

Cuts at:      0      88     704    1019    1145    1254 
   Size:         88     616     315     126     109

  Fragments arranged by size: 

                 616     315     126     109      88
 
SmlI C'TyrA_G

Cuts at:      0     279     689    1254 
   Size:        279     410     565

  Fragments arranged by size: 

                 565     410     279
 
SspI AAT'ATT

Cuts at:      0     353    1254 
   Size:        353     901
 
StyI C'CwwG_G

Cuts at:      0     677    1254 
   Size:        677     577
 
SwaI ATTT'AAAT

Cuts at:      0     498    1254 
   Size:        498     756
 
TatI w'GTAC_w

Cuts at:      0     993    1254 
   Size:        993     261
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1196    1254 
   Size:       1196      58
 
XmnI GAAnn'nnTTC

Cuts at:      0     509    1254 
   Size:        509     745
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI    ApaLI     ApoI    BamHI     BanI     BbsI
 Bme1580I     BpmI    BpuEI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrGI    BstYI     BtgI     BtsI     DraI   DraIII
     EaeI     EarI  Eco57MI    EcoRI    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI    PflMI     PstI    PvuII     SapI     SbfI
     SfcI     SmlI     SspI     StyI     SwaI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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