Restriction map for
pF1KB7381
(Circular) (Six-base) MAPSORT of: pF1KB7381.seq Check: 3293 from: 1 to: 4499
pF1KB7381 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 780 796 780
Size: 16 4483
AccI GT'mk_AC
Cuts at: 519 1537 519
Size: 1018 3481
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1017 1147 2324 2756 3503 3771 679
Size: 338 130 1177 432 747 268 1407
Fragments arranged by size:
1407 1177 747 432 338 268 130
AflIII A'CryG_T
Cuts at: 335 1851 2955 3932 335
Size: 1516 1104 977 902
Fragments arranged by size:
1516 1104 977 902
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 447 1804 3371 447
Size: 1357 1567 1575
Fragments arranged by size:
1575 1567 1357
ApaI G_GGCC'C
Cuts at: 900 1049 1216 1365 1474 900
Size: 149 167 149 109 3925
Fragments arranged by size:
3925 167 149 149 109
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 866 1326 1503 3852 4108 866
Size: 460 177 2349 256 1257
Fragments arranged by size:
2349 1257 460 256 177
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 228 417 1039 1357 1519 228
Size: 189 622 318 162 3208
Fragments arranged by size:
3208 622 318 189 162
BamHI G'GATC_C
Cuts at: 1077 1524 1077
Size: 447 4052
BanI G'GyrC_C
Cuts at: 468 500 802 1515 1929 2177 2212 468
Size: 32 302 713 414 248 35 2755
Fragments arranged by size:
2755 713 414 302 248 35 32
BanII G_rGCy'C
Cuts at: 229 587 760 900 978 1049 1135 1216 1365
Size: 358 173 140 78 71 86 81 149
Cuts at: 1365 1474 1513 2543 229
Size: 109 39 1030 2185
Fragments arranged by size:
2185 1030 358 173 149 140 109 86
81 78 71 39
BbeI G_GCGC'C
Cuts at: 504 1933 2181 504
Size: 1429 248 2822
Fragments arranged by size:
2822 1429 248
BbsI GAAGACnn'nnnn_
Cuts at: 928 928
Size: 4499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 303 337 408 442 90
Size: 34 179 34 71 34 4147
Fragments arranged by size:
4147 179 71 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglII A'GATC_T
Cuts at: 602 2014 4051 602
Size: 1412 2037 1050
Fragments arranged by size:
2037 1412 1050
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 473 807 900 1049 1216 1365 1474 2124 2217
Size: 334 93 149 167 149 109 650 93
Cuts at: 2217 3273 473
Size: 1056 1699
Fragments arranged by size:
1699 1056 650 334 167 149 149 109
93 93
BmgBI CAC'GTC
Cuts at: 402 402
Size: 4499
BmrI ACTGGGnnnn_n'
Cuts at: 282 535 1211 1711 1792 2127 4271 282
Size: 253 676 500 81 335 2144 510
Fragments arranged by size:
2144 676 510 500 335 253 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 774 2866 774
Size: 2092 2407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 469 944 243
Size: 226 475 3798
Fragments arranged by size:
3798 475 226
Bpu10I CC'TnA_GC
Cuts at: 382 586 876 1304 382
Size: 204 290 428 3577
Fragments arranged by size:
3577 428 290 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1675 2554 3046 3344 1157
Size: 518 879 492 298 2312
Fragments arranged by size:
2312 879 518 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4203 4203
Size: 4499
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 501 1930 2178 2880 4376 501
Size: 1429 248 702 1496 624
Fragments arranged by size:
1496 1429 702 624 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 838
Size: 30 4469
BseYI C'CCAG_C
Cuts at: 148 1209 1401 1924 2682 3259 3755 148
Size: 1061 192 523 758 577 496 892
Fragments arranged by size:
1061 892 758 577 523 496 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 574 1024 556
Size: 18 450 4031
Fragments arranged by size:
4031 450 18
BsiEI CG_ry'CG
Cuts at: 104 435 495 2041 2087 3295 104
Size: 331 60 1546 46 1208 1308
Fragments arranged by size:
1546 1308 1208 331 60 46
BsiHKAI G_wGCw'C
Cuts at: 565 587 760 1513 2291 2481 3273 565
Size: 22 173 753 778 190 792 1791
Fragments arranged by size:
1791 792 778 753 190 173 22
BsmI GAATG_Cn'
Cuts at: 1059 1059
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 229 473 565 587 760 807 900 978 1049
Size: 244 92 22 173 47 93 78 71
Cuts at: 1049 1135 1216 1365 1474 1513 2124 2217 2291
Size: 86 81 149 109 39 611 93 74
Cuts at: 2291 2481 2543 3273 229
Size: 190 62 730 1455
Fragments arranged by size:
1455 730 611 244 190 173 149 109
93 93 92 86 81 78 74 71
62 47 39 22
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 780 796 1197 1549 1763 2065 2446 780
Size: 16 401 352 214 302 381 2833
Fragments arranged by size:
2833 401 381 352 302 214 16
BsrBI CCG'CTC
Cuts at: 50 233 1293 2791 2845 4329 4494 50
Size: 183 1060 1498 54 1484 165 55
Fragments arranged by size:
1498 1484 1060 183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 2411 717
Size: 1694 2805
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 543 1876 543
Size: 1333 3166
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 602 1077 1524 1991 2014 2349 2595 3607
Size: 601 475 447 467 23 335 246 1012
Cuts at: 3607 3693 3705 4051 1
Size: 86 12 346 449
Fragments arranged by size:
1012 601 475 467 449 447 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 937 937
Size: 4499
BtgI C'CryG_G
Cuts at: 134 639 134
Size: 505 3994
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 985 1742 985
Size: 757 3742
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 432 492 2084 2258 2649 2676 4021 432
Size: 60 1592 174 391 27 1345 910
Fragments arranged by size:
1592 1345 910 391 174 60 27
EagI C'GGCC_G
Cuts at: 432 492 2084 432
Size: 60 1592 2847
Fragments arranged by size:
2847 1592 60
EarI CTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 585 758 1511 585
Size: 173 753 3573
Fragments arranged by size:
3573 753 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 469 679 944 1017 1147 2324 2756 3503
Size: 226 210 265 73 130 1177 432 747
Cuts at: 3503 3771 243
Size: 268 971
Fragments arranged by size:
1177 971 747 432 268 265 226 210
130 73
EcoNI CCTnn'n_nnAGG
Cuts at: 811 1070 811
Size: 259 4240
EcoO109I rG'GnC_Cy
Cuts at: 145 195 510 816 896 897 1045 1102 1195
Size: 50 315 306 80 1 148 57 93
Cuts at: 1195 1213 1347 1361 1362 1638 145
Size: 18 134 14 1 276 3006
Fragments arranged by size:
3006 315 306 276 148 134 93 80
57 50 18 14 1 1
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 121 187 504 1933 2181 3203 4182 121
Size: 66 317 1429 248 1022 979 438
Fragments arranged by size:
1429 1022 979 438 317 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 627 659 663 695 915 947 2341 2371 2373
Size: 32 4 32 220 32 1394 30 2
Cuts at: 2373 2403 627
Size: 30 2723
Fragments arranged by size:
2723 1394 220 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 520 1538 520
Size: 1018 3481
KasI G'GCGC_C
Cuts at: 500 1929 2177 500
Size: 1429 248 2822
Fragments arranged by size:
2822 1429 248
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3354 3170
Size: 184 4315
MscI TGG'CCA
Cuts at: 2260 2260
Size: 4499
MslI CAynn'nnrTG
Cuts at: 2488 2615 2488
Size: 127 4372
MspA1I CmG'CkG
Cuts at: 124 221 497 1610 1924 2284 3297 3542 124
Size: 97 276 1113 314 360 1013 245 1081
Fragments arranged by size:
1113 1081 1013 360 314 276 245 97
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 501 1930 2178 501
Size: 1429 248 2822
Fragments arranged by size:
2822 1429 248
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NotI GC'GGCC_GC
Cuts at: 432 432
Size: 4499
NspI r_CATG'y
Cuts at: 339 1552 2052 2959 339
Size: 1213 500 907 1879
Fragments arranged by size:
1879 1213 907 500
PasI CC'CwG_GG
Cuts at: 1082 1339 1407 1082
Size: 257 68 4174
Fragments arranged by size:
4174 257 68
PciI A'CATG_T
Cuts at: 335 2955 335
Size: 2620 1879
PfoI T'CCnGG_A
Cuts at: 1013 1013
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 195 510 816 1347 195
Size: 315 306 531 3347
Fragments arranged by size:
3347 531 315 306
PshAI GACnn'nnGTC
Cuts at: 515 515
Size: 4499
PspOMI G'GGCC_C
Cuts at: 896 1045 1212 1361 1470 896
Size: 149 167 149 109 3925
Fragments arranged by size:
3925 167 149 149 109
PstI C_TGCA'G
Cuts at: 815 1194 1546 2231 815
Size: 379 352 685 3083
Fragments arranged by size:
3083 685 379 352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 124 1924 2284 124
Size: 1800 360 2339
Fragments arranged by size:
2339 1800 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 587 760 1513 587
Size: 173 753 3573
Fragments arranged by size:
3573 753 173
SalI G'TCGA_C
Cuts at: 518 1536 518
Size: 1018 3481
SanDI GG'GwC_CC
Cuts at: 195 195
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1194 1546 1194
Size: 352 4147
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SexAI A'CCwGG_T
Cuts at: 798 798
Size: 4499
SfcI C'TryA_G
Cuts at: 33 811 1190 1542 2227 3220 3411 33
Size: 778 379 352 685 993 191 1121
Fragments arranged by size:
1121 993 778 685 379 352 191
SfoI GGC'GCC
Cuts at: 502 1931 2179 502
Size: 1429 248 2822
Fragments arranged by size:
2822 1429 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 1172 1654 2569 3061 3323 1172
Size: 482 915 492 262 2348
Fragments arranged by size:
2348 915 492 482 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StyI C'CwwG_G
Cuts at: 272 1224 1280 1391 1633 1987 272
Size: 952 56 111 242 354 2784
Fragments arranged by size:
2784 952 354 242 111 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2082 2215 2674 2883 2082
Size: 133 459 209 3698
Fragments arranged by size:
3698 459 209 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 400 2296 400
Size: 1896 2603
XbaI T'CTAG_A
Cuts at: 1200 1530 1200
Size: 330 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 682 1994 682
Size: 1312 3187
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI