Restriction map for pF1KB7380
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BciVI       BcnI        BfaI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MaeI        MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AflII         571  CTTAAG               C^TTAAG
AocI          166  CCTGAGG              CC^T[ACGT]AGG
BamHI         979  GGATCC               G^GATCC
BbsI          260  GAAGAC               GAAGAC(2/6)
BcgI          381  CGAGCAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          571  CTTAAG               C^TTAAG
BglII         116  AGATCT               A^GATCT
BpuAI         260  GAAGAC               GAAGAC(2/6)
BsaI          902  GGTCTC               GGTCTC(1/5)
BsaWI          45  TCCGGT               [AT]^CCGG[AT]
BseRI         203  CTCCTC               GAGGAG(10/8)
BsmFI         351  GGGAC                GGGAC(10/14)
BspHI         183  TCATGA               T^CATGA
BsrI          636  CCAGT                ACTGG(1/-1)
BstXI         234  CCAGGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        166  CCTGAGG              CC^T[ACGT]AGG
DraII         351  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eco81I        166  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      351  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         620  GATATC               GAT^ATC
HapII          46  CCGG                 C^CGG
HinfI         370  GATTC                G^A[ACGT]TC
HpaII          46  CCGG                 C^CGG
MaeII         585  ACGT                 A^CGT
MspA1I        671  CAGCTG               C[AC]G^C[GT]G
MspI           46  CCGG                 C^CGG
PflMI         636  CCAGTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         351  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         671  CAGCTG               CAG^CTG
RcaI          183  TCATGA               T^CATGA
SapI          406  GAAGAGC              GCTCTTC(1/4)
TfiI          370  GATTC                G^A[AT]TC
Van91I        636  CCAGTTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          358  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
AfaI          472  GTAC                 GT^AC
              928  GTAC                
AlwI          980  GATCC                GGATC(4/5)
              979  GGATC               
ApoI          358  GAATTT               [GA]^AATT[CT]
              539  AAATTC              
AvaII          51  GGTCC                G^G[AT]CC
              352  GGACC               
BbvCI         990  CCTCAGC              CCTCAGC(-5/-2)
              136  GCTGAGG             
BclI          693  TGATCA               T^GATCA
              779  TGATCA              
BglI          221  GCCTTGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              230  GCCCCCAGGGC         
BsmAI          26  GAGAC                GTCTC(1/5)
              903  GTCTC               
BstF5I         69  CATCC                GGATG(2/0)
              163  CATCC               
BstYI         116  AGATCT               [GA]^GATC[CT]
              979  GGATCC              
Cac8I         384  GCAAGC               GC[ACGT]^[ACGT]GC
              411  GCTTGC              
EarI          406  GAAGAG               CTCTTC(1/4)
              628  GAAGAG              
FbaI          693  TGATCA               T^GATCA
              779  TGATCA              
FokI           69  CATCC                GGATG(9/13)
              163  CATCC               
Ksp632I       406  GAAGAG               CTCTTC(1/4)
              628  GAAGAG              
MflI          116  AGATCT               [GA]^GATC[CT]
              979  GGATCC              
NlaIII        184  CATG                 CATG^
              789  CATG                
RsaI          472  GTAC                 GT^AC
              928  GTAC                
SfaNI         667  GATGC                GCATC(5/9)
              162  GCATC               
SmlI          571  CTTAAG               C^T[CT][GA]AG
              973  CTCAAG              
TaqI          108  TCGA                 T^CGA
              960  TCGA                
TspRI         931  CAGTG                CA[GC]TG[ACGT][ACGT]^
              950  CACTG               
TthHB8I       108  TCGA                 T^CGA
              960  TCGA                
XhoII         116  AGATCT               [GA]^GATC[CT]
              979  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AluI        Asp700I     BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      ItaI        MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       Tru9I       
TseI        Tsp509I     XmnI        
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