Restriction map for
pF1KB7380
(Circular) (Six-base) MAPSORT of: pF1KB7380.seq Check: 7312 from: 1 to: 4121
pF1KB7380 4121 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1159 1159
Size: 4121
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2378 3125 3393 1946
Size: 432 747 268 2674
Fragments arranged by size:
2674 747 432 268
AflII C'TTAA_G
Cuts at: 679 679
Size: 4121
AflIII A'CryG_T
Cuts at: 1473 2577 3554 1473
Size: 1104 977 2040
Fragments arranged by size:
2040 1104 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 1426 2993 1426
Size: 1567 2554
ApaLI G'TGCA_C
Cuts at: 2891 2891
Size: 4121
ApoI r'AATT_y
Cuts at: 466 647 1125 3474 3730 466
Size: 181 478 2349 256 857
Fragments arranged by size:
2349 857 478 256 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 1141 1141
Size: 4121
BamHI G'GATC_C
Cuts at: 1087 1146 1087
Size: 59 4062
BanI G'GyrC_C
Cuts at: 1137 1551 1799 1834 1137
Size: 414 248 35 3424
Fragments arranged by size:
3424 414 248 35
BanII G_rGCy'C
Cuts at: 349 635 1082 1135 2165 349
Size: 286 447 53 1030 2305
Fragments arranged by size:
2305 1030 447 286 53
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 375 375
Size: 4121
BbvCI CC'TCA_GC
Cuts at: 245 1099 245
Size: 854 3267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 512 478
Size: 34 4087
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 801 887 1641 801
Size: 86 754 3281
Fragments arranged by size:
3281 754 86
BglI GCCn_nnn'nGGC
Cuts at: 335 344 335
Size: 9 4112
BglII A'GATC_T
Cuts at: 224 1636 3673 224
Size: 1412 2037 672
Fragments arranged by size:
2037 1412 672
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 1746 1839 2895 1746
Size: 93 1056 2972
Fragments arranged by size:
2972 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 738 1333 1414 1749 3893 738
Size: 595 81 335 2144 966
Fragments arranged by size:
2144 966 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
Bpu10I CC'TnA_GC
Cuts at: 245 1099 245
Size: 854 3267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1297 2176 2668 2966 1066
Size: 231 879 492 298 2221
Fragments arranged by size:
2221 879 492 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 1016 3825 1016
Size: 2809 1312
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 153 1310 1831 2509 2783 2930 3341 153
Size: 1157 521 678 274 147 411 933
Fragments arranged by size:
1157 933 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 302
Size: 4121
BseYI C'CCAG_C
Cuts at: 1546 2304 2881 3377 1546
Size: 758 577 496 2290
Fragments arranged by size:
2290 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1663 1709 2917 104
Size: 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1135 1913 2103 2895 1135
Size: 778 190 792 2361
Fragments arranged by size:
2361 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 349 635 1082 1135 1746 1839 1913 2103 2165
Size: 286 447 53 611 93 74 190 62
Cuts at: 2165 2895 349
Size: 730 1575
Fragments arranged by size:
1575 730 611 447 286 190 93 74
62 53
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 291 3297 291
Size: 3006 1115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 1385 1687 2068 1171
Size: 214 302 381 3224
Fragments arranged by size:
3224 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2413 2467 3951 4116 50
Size: 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 2119 2300 2509 2119
Size: 181 209 3731
Fragments arranged by size:
3731 209 181
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstXI CCAn_nnnn'nTGG
Cuts at: 349 349
Size: 4121
BstYI r'GATC_y
Cuts at: 1 224 1087 1146 1613 1636 1971 2217 3229
Size: 223 863 59 467 23 335 246 1012
Cuts at: 3229 3315 3327 3673 1
Size: 86 12 346 449
Fragments arranged by size:
1012 863 467 449 346 335 246 223
86 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 275 275
Size: 4121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 1057 1364 1057
Size: 307 3814
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 1706 1880 2271 2298 3643 1706
Size: 174 391 27 1345 2184
Fragments arranged by size:
2184 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1706 1706
Size: 4121
EarI CTCTTCn'nnn_
Cuts at: 509 731 2144 2354 509
Size: 222 1413 210 2276
Fragments arranged by size:
2276 1413 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1133 1133
Size: 4121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2378 3125 3393 1946
Size: 432 747 268 2674
Fragments arranged by size:
2674 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 460 1260 460
Size: 800 3321
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
EcoRV GAT'ATC
Cuts at: 730 730
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 436 601 679 2140 436
Size: 165 78 1461 2417
Fragments arranged by size:
2417 1461 165 78
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 1963 1993 1995 2025 209
Size: 32 1722 30 2 30 2305
Fragments arranged by size:
2305 1722 32 30 30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2792 2976 2792
Size: 184 3937
MscI TGG'CCA
Cuts at: 1882 1882
Size: 4121
MslI CAynn'nnrTG
Cuts at: 2110 2237 2110
Size: 127 3994
MspA1I CmG'CkG
Cuts at: 781 1232 1546 1906 2919 3164 781
Size: 451 314 360 1013 245 1738
Fragments arranged by size:
1738 1013 451 360 314 245
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NspI r_CATG'y
Cuts at: 1174 1674 2581 1174
Size: 500 907 2714
Fragments arranged by size:
2714 907 500
PasI CC'CwG_GG
Cuts at: 342 1026 342
Size: 684 3437
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PflMI CCAn_nnn'nTGG
Cuts at: 750 750
Size: 4121
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PpuMI rG'GwC_Cy
Cuts at: 460 460
Size: 4121
PstI C_TGCA'G
Cuts at: 1168 1853 1168
Size: 685 3436
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 781 1546 1906 781
Size: 765 360 2996
Fragments arranged by size:
2996 765 360
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 1135 1135
Size: 4121
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 509 2144 2354 509
Size: 1635 210 2276
Fragments arranged by size:
2276 1635 210
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SfcI C'TryA_G
Cuts at: 33 1164 1849 2842 3033 33
Size: 1131 685 993 191 1121
Fragments arranged by size:
1131 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 679 1081 1276 2191 2683 2945 679
Size: 402 195 915 492 262 1855
Fragments arranged by size:
1855 915 492 402 262 195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
StyI C'CwwG_G
Cuts at: 1255 1609 1255
Size: 354 3767
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 1837 2296 2505 1704
Size: 133 459 209 3320
Fragments arranged by size:
3320 459 209 133
TatI w'GTAC_w
Cuts at: 579 3636 579
Size: 3057 1064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
XmnI GAAnn'nnTTC
Cuts at: 416 419 659 416
Size: 3 240 3878
Fragments arranged by size:
3878 240 3
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI