Restriction map for pF1KB7366
 (Linear) (Six-base) MAPSORT of: KIBB7366.seq  Check: 4936  from: 1  to: 735



KIBB7366.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     292     460     735 
   Size:        292     168     275

  Fragments arranged by size: 

                 292     275     168
 
AfeI AGC'GCT

Cuts at:      0     421     735 
   Size:        421     314
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     176     219     735 
   Size:         30     146      43     516

  Fragments arranged by size: 

                 516     146      43      30
 
ApaI G_GGCC'C

Cuts at:      0     344     519     711     735 
   Size:        344     175     192      24

  Fragments arranged by size: 

                 344     192     175      24
 
AseI AT'TA_AT

Cuts at:      0     497     735 
   Size:        497     238
 
AvaI C'yCGr_G

Cuts at:      0     145     735 
   Size:        145     590
 
BamHI G'GATC_C

Cuts at:      0     347     735 
   Size:        347     388
 
BanII G_rGCy'C

Cuts at:      0     344     519     711     735 
   Size:        344     175     192      24

  Fragments arranged by size: 

                 344     192     175      24
 
Bme1580I G_kGCm'C

Cuts at:      0      66     344     398     519     711     735 
   Size:         66     278      54     121     192      24

  Fragments arranged by size: 

                 278     192     121      66      54      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     264     735 
   Size:        264     471
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     597     735 
   Size:        536      61     138

  Fragments arranged by size: 

                 536     138      61
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     682     735 
   Size:        682      53
 
BseYI C'CCAG_C

Cuts at:      0      65     139     703     735 
   Size:         65      74     564      32

  Fragments arranged by size: 

                 564      74      65      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     189     205     735 
   Size:        180       9      16     530

  Fragments arranged by size: 

                 530     180      16       9
 
BsiEI CG_ry'CG

Cuts at:      0     512     621     735 
   Size:        512     109     114

  Fragments arranged by size: 

                 512     114     109
 
BsiHKAI G_wGCw'C

Cuts at:      0     328     735 
   Size:        328     407
 
BsmI GAATG_Cn'

Cuts at:      0     192     383     705     735 
   Size:        192     191     322      30

  Fragments arranged by size: 

                 322     192     191      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     719     735 
   Size:        719      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     328     344     398     519     711     735 
   Size:         66     262      16      54     121     192      24

  Fragments arranged by size: 

                 262     192     121      66      54      24      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     292     345     460     735 
   Size:        104     188      53     115     275

  Fragments arranged by size: 

                 275     188     115     104      53
 
BsrBI CCG'CTC

Cuts at:      0     617     735 
   Size:        617     118
 
BsrGI T'GTAC_A

Cuts at:      0     242     735 
   Size:        242     493
 
BssSI C'ACGA_G

Cuts at:      0     716     735 
   Size:        716      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      21      30     735 
   Size:         21       9     705

  Fragments arranged by size: 

                 705      21       9
 
BstYI r'GATC_y

Cuts at:      0     347     735 
   Size:        347     388
 
Bsu36I CC'TnA_GG

Cuts at:      0     489     735 
   Size:        489     246
 
EaeI y'GGCC_r

Cuts at:      0     162     200     509     618     674     692     735 
   Size:        162      38     309     109      56      18      43

  Fragments arranged by size: 

                 309     162     109      56      43      38      18
 
EagI C'GGCC_G

Cuts at:      0     509     618     735 
   Size:        509     109     117

  Fragments arranged by size: 

                 509     117     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     597     735 
   Size:        536      61     138

  Fragments arranged by size: 

                 536     138      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     340     341     515     516     707     708     735 
   Size:        340       1     174       1     191       1      27

  Fragments arranged by size: 

                 340     191     174      27       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     312     735 
   Size:        312     423
 
HaeII r_GCGC'y

Cuts at:      0     294     423     735 
   Size:        294     129     312

  Fragments arranged by size: 

                 312     294     129
 
HincII GTy'rAC

Cuts at:      0     360     735 
   Size:        360     375
 
MscI TGG'CCA

Cuts at:      0     164     676     735 
   Size:        164     512      59

  Fragments arranged by size: 

                 512     164      59
 
MslI CAynn'nnrTG

Cuts at:      0     631     735 
   Size:        631     104
 
MspA1I CmG'CkG

Cuts at:      0      27     311     623     735 
   Size:         27     284     312     112

  Fragments arranged by size: 

                 312     284     112      27
 
NotI GC'GGCC_GC

Cuts at:      0     618     735 
   Size:        618     117
 
PasI CC'CwG_GG

Cuts at:      0     651     735 
   Size:        651      84
 
PfoI T'CCnGG_A

Cuts at:      0     302     735 
   Size:        302     433
 
PshAI GACnn'nnGTC

Cuts at:      0     311     735 
   Size:        311     424
 
PspOMI G'GGCC_C

Cuts at:      0     340     515     707     735 
   Size:        340     175     192      28

  Fragments arranged by size: 

                 340     192     175      28
 
PstI C_TGCA'G

Cuts at:      0     526     735 
   Size:        526     209
 
PvuII CAG'CTG

Cuts at:      0      27     311     735 
   Size:         27     284     424

  Fragments arranged by size: 

                 424     284      27
 
SfcI C'TryA_G

Cuts at:      0     522     603     735 
   Size:        522      81     132

  Fragments arranged by size: 

                 522     132      81
 
StuI AGG'CCT

Cuts at:      0     572     735 
   Size:        572     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     345     735 
   Size:        345     390
 
TatI w'GTAC_w

Cuts at:      0     242     735 
   Size:        242     493
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     669     735 
   Size:        669      66
 

 Enzymes that do cut:

     AarI     AfeI    AlwNI     ApaI     AseI     AvaI    BamHI    BanII
 Bme1580I     BmrI     BpmI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrGI    BssSI   BstAPI
    BstYI   Bsu36I     EaeI     EagI  Eco57MI EcoO109I     FalI    HaeII
   HincII     MscI     MslI   MspA1I     NotI     PasI     PfoI    PshAI
   PspOMI     PstI    PvuII     SfcI     StuI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI    AsiSI
    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiWI    BspEI    BspHI    BsrDI    BsrFI   BssHII    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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