Restriction map for pF1KB7363
 (Linear) (Six-base) MAPSORT of: KIBB7363.seq  Check: 3819  from: 1  to: 1086



KIBB7363.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

Acc65I G'GTAC_C

Cuts at:      0    1079    1086 
   Size:       1079       7
 
AleI CACnn'nnGTG

Cuts at:      0     361    1086 
   Size:        361     725
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     661    1086 
   Size:        661     425
 
AlwNI CAG_nnn'CTG

Cuts at:      0     841    1086 
   Size:        841     245
 
ApaI G_GGCC'C

Cuts at:      0    1051    1086 
   Size:       1051      35
 
ApoI r'AATT_y

Cuts at:      0     503     733    1086 
   Size:        503     230     353

  Fragments arranged by size: 

                 503     353     230
 
AvaI C'yCGr_G

Cuts at:      0     587     599     759     778     978    1086 
   Size:        587      12     160      19     200     108

  Fragments arranged by size: 

                 587     200     160     108      19      12
 
BanI G'GyrC_C

Cuts at:      0     443    1079    1086 
   Size:        443     636       7

  Fragments arranged by size: 

                 636     443       7
 
BanII G_rGCy'C

Cuts at:      0     254     583     956    1051    1086 
   Size:        254     329     373      95      35

  Fragments arranged by size: 

                 373     329     254      95      35
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1011    1045    1086 
   Size:       1011      34      41

  Fragments arranged by size: 

                1011      41      34
 
BglII A'GATC_T

Cuts at:      0     329    1086 
   Size:        329     757
 
BlpI GC'TnA_GC

Cuts at:      0     551     582    1086 
   Size:        551      31     504

  Fragments arranged by size: 

                 551     504      31
 
Bme1580I G_kGCm'C

Cuts at:      0    1051    1086 
   Size:       1051      35
 
BmgBI CAC'GTC

Cuts at:      0     372    1086 
   Size:        372     714
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     763    1086 
   Size:        763     323
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     273    1086 
   Size:        273     813
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1086 
   Size:        872     214
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     602    1086 
   Size:        602     484
 
BsaBI GATnn'nnATC

Cuts at:      0      67    1086 
   Size:         67    1019
 
BsaHI Gr'CG_yC

Cuts at:      0     410     623    1086 
   Size:        410     213     463

  Fragments arranged by size: 

                 463     410     213
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511     553     583     864     894    1086 
   Size:        481      30      42      30     281      30     192

  Fragments arranged by size: 

                 481     281     192      42      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0      17     633     648     901    1018    1086 
   Size:         17     616      15     253     117      68

  Fragments arranged by size: 

                 616     253     117      68      17      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     254     300     956    1086 
   Size:        254      46     656     130

  Fragments arranged by size: 

                 656     254     130      46
 
Bsp1286I G_dGCh'C

Cuts at:      0     254     300     583     956    1051    1086 
   Size:        254      46     283     373      95      35

  Fragments arranged by size: 

                 373     283     254      95      46      35
 
BspHI T'CATG_A

Cuts at:      0     513    1086 
   Size:        513     573
 
BsrBI CCG'CTC

Cuts at:      0     747    1086 
   Size:        747     339
 
BsrFI r'CCGG_y

Cuts at:      0     440    1024    1086 
   Size:        440     584      62

  Fragments arranged by size: 

                 584     440      62
 
BssSI C'ACGA_G

Cuts at:      0     247    1086 
   Size:        247     839
 
BstEII G'GTnAC_C

Cuts at:      0     652    1086 
   Size:        652     434
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1042    1086 
   Size:       1042      44
 
BstYI r'GATC_y

Cuts at:      0     175     329    1086 
   Size:        175     154     757

  Fragments arranged by size: 

                 757     175     154
 
Bsu36I CC'TnA_GG

Cuts at:      0     275    1086 
   Size:        275     811
 
BtgI C'CryG_G

Cuts at:      0     359     944    1086 
   Size:        359     585     142

  Fragments arranged by size: 

                 585     359     142
 
BtsI GCAGTG_nn'

Cuts at:      0     800    1086 
   Size:        800     286
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     982    1086 
   Size:        982     104
 
EaeI y'GGCC_r

Cuts at:      0     120     947     965    1032    1086 
   Size:        120     827      18      67      54

  Fragments arranged by size: 

                 827     120      67      54      18
 
EcoICRI GAG'CTC

Cuts at:      0     252     954    1086 
   Size:        252     702     132

  Fragments arranged by size: 

                 702     252     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1086 
   Size:        872     214
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159    1047    1048    1086 
   Size:        159     888       1      38

  Fragments arranged by size: 

                 888     159      38       1
 
EcoRI G'AATT_C

Cuts at:      0     733    1086 
   Size:        733     353
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     300     348    1086 
   Size:        300      48     738

  Fragments arranged by size: 

                 738     300      48
 
HaeII r_GCGC'y

Cuts at:      0     767    1004    1086 
   Size:        767     237      82

  Fragments arranged by size: 

                 767     237      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     300     332     553     585     846     864     878     896 
   Size:        300      32     221      32     261      18      14      18

Cuts at:    896    1086 
   Size:        190

  Fragments arranged by size: 

                 300     261     221     190      32      32      18      18
                  14
 
KpnI G_GTAC'C

Cuts at:      0    1083    1086 
   Size:       1083       3
 
MfeI C'AATT_G

Cuts at:      0     266    1086 
   Size:        266     820
 
MslI CAynn'nnrTG

Cuts at:      0     202     361     418     512     643    1086 
   Size:        202     159      57      94     131     443

  Fragments arranged by size: 

                 443     202     159     131      94      57
 
MspA1I CmG'CkG

Cuts at:      0     829     838    1086 
   Size:        829       9     248

  Fragments arranged by size: 

                 829     248       9
 
NcoI C'CATG_G

Cuts at:      0     944    1086 
   Size:        944     142
 
NspI r_CATG'y

Cuts at:      0     648    1086 
   Size:        648     438
 
PpuMI rG'GwC_Cy

Cuts at:      0     159    1086 
   Size:        159     927
 
PspOMI G'GGCC_C

Cuts at:      0    1047    1086 
   Size:       1047      39
 
SacI G_AGCT'C

Cuts at:      0     254     956    1086 
   Size:        254     702     130

  Fragments arranged by size: 

                 702     254     130
 
SanDI GG'GwC_CC

Cuts at:      0     159    1086 
   Size:        159     927
 
SmaI CCC'GGG

Cuts at:      0     589    1086 
   Size:        589     497
 
SmlI C'TyrA_G

Cuts at:      0     599    1086 
   Size:        599     487
 
SphI G_CATG'C

Cuts at:      0     648    1086 
   Size:        648     438
 
SspI AAT'ATT

Cuts at:      0     382    1086 
   Size:        382     704
 
StuI AGG'CCT

Cuts at:      0     701    1086 
   Size:        701     385
 
StyI C'CwwG_G

Cuts at:      0     944    1086 
   Size:        944     142
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     481     513    1086 
   Size:        481      32     573

  Fragments arranged by size: 

                 573     481      32
 
XhoI C'TCGA_G

Cuts at:      0     599    1086 
   Size:        599     487
 
XmaI C'CCGG_G

Cuts at:      0     587    1086 
   Size:        587     499
 

 Enzymes that do cut:

   Acc65I     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BcgI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI Bsp1286I
    BspHI    BsrBI    BsrFI    BssSI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DrdI     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI    HaeII    Hin4I     KpnI     MfeI     MslI   MspA1I     NcoI
     NspI    PpuMI   PspOMI     SacI    SanDI     SmaI     SmlI     SphI
     SspI     StuI     StyI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI     BmtI   Bpu10I    BpuEI    BsaAI    BsaWI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EarI     EciI    EcoNI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XmnI     ZraI
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