Restriction map for pF1KB7301
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BspLU11I    BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     CelII       Cfr10I      CpoI        DraIII      
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HgaI        
HindIII     KpnI        KspI        MfeI        MluI        
MluNI       MscI        MslI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII        1251  GACGTC               GACGT^C
AccI         1312  GTAGAC               GT^[AC][GT]AC
AccIII       1326  TCCGGA               T^CCGGA
AciI           33  GCGG                 CCGC(-3/-1)
AflIII       1039  ACACGT               A^C[GA][CT]GT
AhdI         1246  GACTGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1438  CCTAAGG              CC^T[ACGT]AGG
AseI         2241  ATTAAT               AT^TAAT
AsnI         2241  ATTAAT               AT^TAAT
Asp700I      2160  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1246  GACTGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        2188  GAGCAC               G[AT]GC[AT]^C
AvaI           57  CCCGAG               C^[CT]CG[GA]G
BbeI          170  GGCGCC               GGCGC^C
BclI         1542  TGATCA               T^GATCA
BcnI         2381  CCCGG                CC^[GC]GG
BglII        1395  AGATCT               A^GATCT
BmyI         2188  GAGCAC               G[AGT]GC[ACT]^C
BsaBI         232  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1326  TCCGGA               [AT]^CCGG[AT]
BseAI        1326  TCCGGA               T^CCGGA
BsgI         1841  GTGCAG               GTGCAG(16/14)
BsiHKAI      2188  GAGCAC               G[AT]GC[AT]^C
BsiYI        1120  CCCAAAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1120  CCCAAAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI          57  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     2188  GAGCAC               G[AGT]GC[ACT]^C
Bsp1407I     2294  TGTACA               T^GTACA
BspDI         313  ATCGAT               AT^CGAT
BspEI        1326  TCCGGA               T^CCGGA
BspMI        1758  GCAGGT               ACCTGC(4/8)
BsrGI        2294  TGTACA               T^GTACA
BssSI        1022  CTCGTG               CACGAG(-5/-1)
BstAPI       1749  GCAGCTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1438  CCTAAGG              CC^T[ACGT]AGG
CfoI          171  GCGC                 GCG^C
ClaI          313  ATCGAT               AT^CGAT
DraII          53  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          155  CCGTGG               C^C[GA][CT]GG
Eam1105I     1246  GACTGGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1438  CCTAAGG              CC^T[ACGT]AGG
EcoO109I       53  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I      1891  CCTTGG               C^C[AT][AT]GG
FbaI         1542  TGATCA               T^GATCA
HaeII         170  GGCGCC               [GA]GCGC^[CT]
HhaI          171  GCGC                 GCG^C
HinP1I        171  GCGC                 G^CGC
HincII       2269  GTTAAC               GT[CT]^[GA]AC
HindII       2269  GTTAAC               GT[CT]^[GA]AC
HpaI         2269  GTTAAC               GTT^AAC
KasI          170  GGCGCC               G^GCGCC
MamI          232  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1326  TCCGGA               T^CCGGA
MspA1I         31  CAGCGG               C[AC]G^C[GT]G
NarI          170  GGCGCC               GG^CGCC
NciI         2381  CCCGG                CC^[GC]GG
NspI          990  GCATGC               [GA]CATG^[CT]
PpuMI          53  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2241  ATTAAT               AT^TAAT
ScaI         1295  AGTACT               AGT^ACT
SfoI          170  GGCGCC               GGC^GCC
SmlI         2368  CTTGAG               C^T[CT][GA]AG
SpeI          931  ACTAGT               A^CTAGT
SphI          990  GCATGC               GCATG^C
SspBI        2294  TGTACA               T^GTACA
StyI         1891  CCTTGG               C^C[AT][AT]GG
XbaI         1255  TCTAGA               T^CTAGA
XcmI         1237  CCAGAAAAAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2160  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI          170  GGCGCC               G[GA]^CG[CT]C
             1251  GACGTC              
AfaI         1296  GTAC                 GT^AC
             2295  GTAC                
AlwNI         224  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2194  CAGAATCTG           
BanI          170  GGCGCC               G^G[CT][GA]CC
              571  GGCACC              
BpmI         2081  CTCCAG               CTGGAG(16/14)
              715  CTGGAG              
BsaHI         170  GGCGCC               G[GA]^CG[CT]C
             1251  GACGTC              
BsaJI         155  CCGTGG               C^C[ACGT][ACGT]GG
             1891  CCTTGG              
BsmAI         659  GAGAC                GTCTC(1/5)
             1537  GAGAC               
BsrDI        1501  GCAATG               GCAATG(2/0)
             2290  GCAATG              
BstNI        1365  CCTGG                CC^[AT]GG
             1825  CCAGG               
BstYI        1395  AGATCT               [GA]^GATC[CT]
             2328  AGATCC              
Cac8I         990  GCATGC               GC[ACGT]^[ACGT]GC
             1095  GCTGGC              
DraI         1114  TTTAAA               TTT^AAA
             1576  TTTAAA              
EcoRII       1365  CCTGG                ^CC[AT]GG
             1825  CCAGG               
HapII        1327  CCGG                 C^CGG
             2382  CCGG                
Hin1I         170  GGCGCC               G[GA]^CG[CT]C
             1251  GACGTC              
HpaII        1327  CCGG                 C^CGG
             2382  CCGG                
MaeII        1041  ACGT                 A^CGT
             1252  ACGT                
MflI         1395  AGATCT               [GA]^GATC[CT]
             2328  AGATCC              
MspI         1327  CCGG                 C^CGG
             2382  CCGG                
MvaI         1365  CCTGG                CC^[AT]GG
             1825  CCAGG               
RsaI         1296  GTAC                 GT^AC
             2295  GTAC                
SapI         1186  GAAGAGC              GCTCTTC(1/4)
             1585  GAAGAGC             
SspI         1134  AATATT               AAT^ATT
             1484  AATATT              
XhoII        1395  AGATCT               [GA]^GATC[CT]
             2328  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        AvaII       
BbvI        BfaI        BseRI       BspHI       BsrI        
BssKI       BstF5I      Cfr13I      DdeI        DpnII       
EarI        Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RcaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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