Restriction map for pF1KB7301
 (Linear) (Six-base) MAPSORT of: KIBB7301.seq  Check: 2702  from: 1  to: 2553



KIBB7301.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AatII G_ACGT'C

Cuts at:      0    1255    2553 
   Size:       1255    1298
 
AccI GT'mk_AC

Cuts at:      0    1313    2553 
   Size:       1313    1240
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     779     959    1142    1247    1312    1979    2039    2553 
   Size:        779     180     183     105      65     667      60     514

  Fragments arranged by size: 

                 779     667     514     183     180     105      65      60
            
 
AflIII A'CryG_T

Cuts at:      0    1039    2553 
   Size:       1039    1514
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1251    2553 
   Size:       1251    1302
 
AlwNI CAG_nnn'CTG

Cuts at:      0     229    2199    2553 
   Size:        229    1970     354

  Fragments arranged by size: 

                1970     354     229
 
ApoI r'AATT_y

Cuts at:      0     433     478    1162    1343    1369    1461    2553 
   Size:        433      45     684     181      26      92    1092

  Fragments arranged by size: 

                1092     684     433     181      92      45      26
 
AseI AT'TA_AT

Cuts at:      0    2242    2553 
   Size:       2242     311
 
AvaI C'yCGr_G

Cuts at:      0      57    2553 
   Size:         57    2496
 
BanI G'GyrC_C

Cuts at:      0     170     571    2553 
   Size:        170     401    1982

  Fragments arranged by size: 

                1982     401     170
 
BbeI G_GCGC'C

Cuts at:      0     174    2553 
   Size:        174    2379
 
BclI T'GATC_A

Cuts at:      0    1542    2553 
   Size:       1542    1011
 
BglII A'GATC_T

Cuts at:      0    1395    2553 
   Size:       1395    1158
 
BmgBI CAC'GTC

Cuts at:      0    1042    2553 
   Size:       1042    1511
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1077    2553 
   Size:       1077    1476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736    2066    2553 
   Size:        736    1330     487

  Fragments arranged by size: 

                1330     736     487
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2389    2553 
   Size:       2389     164
 
BsaBI GATnn'nnATC

Cuts at:      0     236    2553 
   Size:        236    2317
 
BsaHI Gr'CG_yC

Cuts at:      0     171    1252    2553 
   Size:        171    1081    1301

  Fragments arranged by size: 

                1301    1081     171
 
BsaWI w'CCGG_w

Cuts at:      0    1326    2553 
   Size:       1326    1227
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1667    1697    2553 
   Size:       1667      30     856

  Fragments arranged by size: 

                1667     856      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     844     862    1010    1657    1669    2386    2553 
   Size:        844      18     148     647      12     717     167

  Fragments arranged by size: 

                 844     717     647     167     148      18      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1862    2553 
   Size:       1862     691
 
BsiHKAI G_wGCw'C

Cuts at:      0    2192    2553 
   Size:       2192     361
 
Bsp1286I G_dGCh'C

Cuts at:      0    2192    2553 
   Size:       2192     361
 
BspEI T'CCGG_A

Cuts at:      0    1326    2553 
   Size:       1326    1227
 
BspHI T'CATG_A

Cuts at:      0     126    1545    2225    2553 
   Size:        126    1419     680     328

  Fragments arranged by size: 

                1419     680     328     126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1749    2553 
   Size:       1749     804
 
BsrDI GCAATG_nn'

Cuts at:      0    1508    2297    2553 
   Size:       1508     789     256

  Fragments arranged by size: 

                1508     789     256
 
BsrGI T'GTAC_A

Cuts at:      0    2294    2553 
   Size:       2294     259
 
BssSI C'ACGA_G

Cuts at:      0    1022    2553 
   Size:       1022    1531
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1755    2553 
   Size:       1755     798
 
BstYI r'GATC_y

Cuts at:      0    1395    2328    2553 
   Size:       1395     933     225

  Fragments arranged by size: 

                1395     933     225
 
Bsu36I CC'TnA_GG

Cuts at:      0    1439    2553 
   Size:       1439    1114
 
BtgI C'CryG_G

Cuts at:      0     155    2553 
   Size:        155    2398
 
ClaI AT'CG_AT

Cuts at:      0     314    2553 
   Size:        314    2239
 
DraI TTT'AAA

Cuts at:      0    1116    1578    2553 
   Size:       1116     462     975

  Fragments arranged by size: 

                1116     975     462
 
EarI CTCTTCn'nnn_

Cuts at:      0     540     615     821     839    1181    1580    1634    1646 
   Size:        540      75     206      18     342     399      54      12

Cuts at:   1646    1841    1955    1967    2553 
   Size:        195     114      12     586

  Fragments arranged by size: 

                 586     540     399     342     206     195     114      75
                  54      18      12      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     779     959    1142    1247    1312    1979    2039 
   Size:        736      43     180     183     105      65     667      60

Cuts at:   2039    2066    2553 
   Size:         27     487

  Fragments arranged by size: 

                 736     667     487     183     180     105      65      60
                  43      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54    2553 
   Size:         54    2499
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1996    2181    2553 
   Size:       1996     185     372

  Fragments arranged by size: 

                1996     372     185
 
HaeII r_GCGC'y

Cuts at:      0     174    2553 
   Size:        174    2379
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     745     777    1026    1058    1237    1260    1269    1292 
   Size:        745      32     249      32     179      23       9      23

Cuts at:   1292    1435    1467    2062    2094    2553 
   Size:        143      32     595      32     459

  Fragments arranged by size: 

                 745     595     459     249     179     143      32      32
                  32      32      23      23       9
 
HincII GTy'rAC

Cuts at:      0    2271    2553 
   Size:       2271     282
 
HpaI GTT'AAC

Cuts at:      0    2271    2553 
   Size:       2271     282
 
KasI G'GCGC_C

Cuts at:      0     170    2553 
   Size:        170    2383
 
MspA1I CmG'CkG

Cuts at:      0      33    2553 
   Size:         33    2520
 
NarI GG'CG_CC

Cuts at:      0     171    2553 
   Size:        171    2382
 
NspI r_CATG'y

Cuts at:      0     994    2553 
   Size:        994    1559
 
PpuMI rG'GwC_Cy

Cuts at:      0      54    2553 
   Size:         54    2499
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1181    1580    2553 
   Size:       1181     399     973

  Fragments arranged by size: 

                1181     973     399
 
ScaI AGT'ACT

Cuts at:      0    1297    2553 
   Size:       1297    1256
 
SfcI C'TryA_G

Cuts at:      0     191     401     527    2553 
   Size:        191     210     126    2026

  Fragments arranged by size: 

                2026     210     191     126
 
SfoI GGC'GCC

Cuts at:      0     172    2553 
   Size:        172    2381
 
SmlI C'TyrA_G

Cuts at:      0    2368    2553 
   Size:       2368     185
 
SpeI A'CTAG_T

Cuts at:      0     931    2553 
   Size:        931    1622
 
SphI G_CATG'C

Cuts at:      0     994    2553 
   Size:        994    1559
 
SspI AAT'ATT

Cuts at:      0    1136    1486    2553 
   Size:       1136     350    1067

  Fragments arranged by size: 

                1136    1067     350
 
StyI C'CwwG_G

Cuts at:      0    1891    2553 
   Size:       1891     662
 
TatI w'GTAC_w

Cuts at:      0    1295    2294    2553 
   Size:       1295     999     259

  Fragments arranged by size: 

                1295     999     259
 
XbaI T'CTAG_A

Cuts at:      0    1255    2553 
   Size:       1255    1298
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1244    2553 
   Size:       1244    1309
 
XmnI GAAnn'nnTTC

Cuts at:      0    2164    2553 
   Size:       2164     389
 
ZraI GAC'GTC

Cuts at:      0    1253    2553 
   Size:       1253    1300
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AhdI    AlwNI     ApoI     AseI
     AvaI     BanI     BbeI     BclI    BglII    BmgBI     BmrI     BpmI
    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI
    BstYI   Bsu36I     BtgI     ClaI     DraI     EarI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I   HincII     HpaI     KasI   MspA1I     NarI
     NspI    PpuMI     SapI     ScaI     SfcI     SfoI     SmlI     SpeI
     SphI     SspI     StyI     TatI     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I     BmtI     BplI   Bpu10I     BsaI    BsaAI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BsrBI    BsrFI   BssHII    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com