Restriction map for pF1KB7297
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BfaI        
BfrI        BglII       BlnI        BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SnaBI       SpeI        Sse8387I    SwaI        
TfiI        Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AflIII        609  ACATGT               A^C[GA][CT]GT
AspI         3040  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1656  TGGCCA               TGG^CCA
BbvCI        1181  CCTCAGC              CCTCAGC(-5/-2)
BclI          932  TGATCA               T^GATCA
BsiEI        3237  CGATCG               CG[GA][CT]^CG
Bsp1407I      458  TGTACA               T^GTACA
BspLU11I      609  ACATGT               A^CATGT
BsrGI         458  TGTACA               T^GTACA
BssHII         14  GCGCGC               G^CGCGC
BstEII       2286  GGTCACC              G^GT[ACGT]ACC
BstPI        2286  GGTCACC              G^GT[ACGT]ACC
EarI         2682  GAAGAG               CTCTTC(1/4)
EcoO65I      2286  GGTCACC              G^GT[ACGT]ACC
EcoRV        3177  GATATC               GAT^ATC
FbaI          932  TGATCA               T^GATCA
Ksp632I      2682  GAAGAG               CTCTTC(1/4)
KspI           62  CCGCGG               CCGC^GG
MluNI        1656  TGGCCA               TGG^CCA
MscI         1656  TGGCCA               TGG^CCA
MseI         3170  TTAA                 T^TAA
NdeI         1652  CATATG               CA^TATG
PflFI        3040  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI         3237  CGATCG               CGAT^CG
SacII          62  CCGCGG               CCGC^GG
SgrAI        2701  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         3559  CCCGGG               CCC^GGG
SphI          756  GCATGC               GCATG^C
SspBI         458  TGTACA               T^GTACA
SspI          913  AATATT               AAT^ATT
Tru9I        3170  TTAA                 T^TAA
Tth111I      3040  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        3559  CCCGGG               C^CCGGG
XmaI         3559  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I       1595  GTGCAC               G^TGCAC
             1885  GTGCAC              
ApaLI        1595  GTGCAC               G^TGCAC
             1885  GTGCAC              
BamHI         391  GGATCC               G^GATCC
             2606  GGATCC              
BbeI          305  GGCGCC               GGCGC^C
             1284  GGCGCC              
BbrPI         163  CACGTG               CAC^GTG
             1213  CACGTG              
BbsI         3628  GAAGAC               GAAGAC(2/6)
             3214  GTCTTC              
BcgI         2902  CGAGTGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              999  GCAGGTGTGTCG        
BciVI        2804  GGATAC               GTATCC(6/5)
             3474  GGATAC              
BlpI         1048  GCTGAGC              GC^T[ACGT]AGC
             1344  GCTCAGC             
Bpu1102I     1048  GCTGAGC              GC^T[ACGT]AGC
             1344  GCTCAGC             
BpuAI        3628  GAAGAC               GAAGAC(2/6)
             3214  GTCTTC              
BsaAI         163  CACGTG               [CT]AC^GT[GA]
             1213  CACGTG              
BsrBI         571  GAGCGG               CCGCTC(-3/-3)
              742  GAGCGG              
BsrDI        2424  CATTGC               GCAATG(2/0)
             3019  CATTGC              
CelII        1048  GCTGAGC              GC^T[ACGT]AGC
             1344  GCTCAGC             
EcoNI         993  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1290  CCTGGTGCAGG         
KasI          305  GGCGCC               G^GCGCC
             1284  GGCGCC              
NarI          305  GGCGCC               GG^CGCC
             1284  GGCGCC              
NcoI          822  CCATGG               C^CATGG
             2348  CCATGG              
PmaCI         163  CACGTG               CAC^GTG
             1213  CACGTG              
PmlI          163  CACGTG               CAC^GTG
             1213  CACGTG              
SfoI          305  GGCGCC               GGC^GCC
             1284  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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