Restriction map for pF1KB7293
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AvrII       BamHI       BbeI        BciVI       
BfaI        BfrI        BlnI        BsaWI       BseAI       
BsiWI       BsmI        BspEI       BspLU11I    BsrBI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MroI        
MunI        NarI        NcoI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        1359  CGCG                 CG^CG
AflIII       2342  ACGTGT               A^C[GA][CT]GT
AhdI         3032  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1287  GTGCAC               G^TGCAC
ApaLI        1287  GTGCAC               G^TGCAC
AspEI        3032  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3012  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         158  TGCGCA               TGC^GCA
BbrPI        2026  CACGTG               CAC^GTG
BcgI         1013  GCACCTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1367  TGATCA               T^GATCA
BglII        2846  AGATCT               A^GATCT
BsaAI        2026  CACGTG               [CT]AC^GT[GA]
BsaBI         516  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         179  CGATCG               CG[GA][CT]^CG
Bsp1407I     2993  TGTACA               T^GTACA
BspDI         181  ATCGAT               AT^CGAT
BspHI        2318  TCATGA               T^CATGA
BsrFI         320  GCCGGC               [GA]^CCGG[CT]
BsrGI        2993  TGTACA               T^GTACA
BssSI         331  CACGAG               CACGAG(-5/-1)
BstUI        1359  CGCG                 CG^CG
Cfr10I        320  GCCGGC               [GA]^CCGG[CT]
ClaI          181  ATCGAT               AT^CGAT
DrdI          967  GACATTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3032  GACCTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1789  CTCTTC               CTCTTC(1/4)
FbaI         1367  TGATCA               T^GATCA
FspI          158  TGCGCA               TGC^GCA
HincII       2815  GTCAAC               GT[CT]^[GA]AC
HindII       2815  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1789  CTCTTC               CTCTTC(1/4)
MamI          516  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1359  CGCG                 CG^CG
NaeI          320  GCCGGC               GCC^GGC
NdeI          197  CATATG               CA^TATG
NgoMIV        320  GCCGGC               G^CCGGC
PflFI        3012  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2026  CACGTG               CAC^GTG
PmlI         2026  CACGTG               CAC^GTG
PstI         1260  CTGCAG               CTGCA^G
PvuI          179  CGATCG               CGAT^CG
RcaI         2318  TCATGA               T^CATGA
SapI         1788  GCTCTTC              GCTCTTC(1/4)
SexAI        1753  ACCTGGT              A^CC[AT]GGT
SspBI        2993  TGTACA               T^GTACA
TaqI          182  TCGA                 T^CGA
Tsp509I      1831  AATT                 ^AATT
Tth111I      3012  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       182  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

ApaI          633  GGGCCC               GGGCC^C
             1948  GGGCCC              
BbvCI        1872  CCTCAGC              CCTCAGC(-5/-2)
             2004  CCTCAGC             
BglI          990  GCCTGACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1307  GCCACTACGGC         
BsgI          265  CTGCAC               GTGCAG(16/14)
               45  GTGCAG              
BsmBI          22  GAGACG               CGTCTC(1/5)
             2930  GAGACG              
BsrDI         786  CATTGC               GCAATG(2/0)
             2136  CATTGC              
BstAPI        146  GCACCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2049  GCAGAGGATGC         
BstEII        540  GGTGACC              G^GT[ACGT]ACC
             1084  GGTGACC             
BstPI         540  GGTGACC              G^GT[ACGT]ACC
             1084  GGTGACC             
EcoO65I       540  GGTGACC              G^GT[ACGT]ACC
             1084  GGTGACC             
SacI         1570  GAGCTC               GAGCT^C
             2062  GAGCTC              
SfcI         1103  CTATAG               C^T[GA][CT]AG
             1260  CTGCAG              
SmlI          277  CTCAAG               C^T[CT][GA]AG
             1090  CTCAAG              
TfiI          429  GATTC                G^A[AT]TC
             2693  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SphI        
StuI        StyI        Tru9I       TseI        Tsp45I      
TspRI       Van91I      XcmI        XhoII       
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