Restriction map for pF1KB7293
 (Linear) (Six-base) MAPSORT of: KIBB7293.seq  Check: 1937  from: 1  to: 3090



KIBB7293.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     305    2883    3090 
   Size:        305    2578     207

  Fragments arranged by size: 

                2578     305     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     936    1617    3090 
   Size:        936     681    1473

  Fragments arranged by size: 

                1473     936     681
 
AflIII A'CryG_T

Cuts at:      0    2342    3090 
   Size:       2342     748
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3037    3090 
   Size:       3037      53
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     313    3090 
   Size:        313    2777
 
AlwNI CAG_nnn'CTG

Cuts at:      0     861    1935    2437    3090 
   Size:        861    1074     502     653

  Fragments arranged by size: 

                1074     861     653     502
 
ApaI G_GGCC'C

Cuts at:      0     637    1952    3090 
   Size:        637    1315    1138

  Fragments arranged by size: 

                1315    1138     637
 
ApaLI G'TGCA_C

Cuts at:      0    1287    3090 
   Size:       1287    1803
 
AvaI C'yCGr_G

Cuts at:      0     388    2188    2735    2927    3090 
   Size:        388    1800     547     192     163

  Fragments arranged by size: 

                1800     547     388     192     163
 
BanI G'GyrC_C

Cuts at:      0     189     367     604     750     987    1012    1240    1987 
   Size:        189     178     237     146     237      25     228     747

Cuts at:   1987    2419    3090 
   Size:        432     671

  Fragments arranged by size: 

                 747     671     432     237     237     228     189     178
                 146      25
 
BanII G_rGCy'C

Cuts at:      0      92     236     637     652    1514    1574    1840    1952 
   Size:         92     144     401      15     862      60     266     112

Cuts at:   1952    2066    3090 
   Size:        114    1024

  Fragments arranged by size: 

                1024     862     401     266     144     114     112      92
                  60      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1428    1910    2270    2518    3090 
   Size:       1428     482     360     248     572

  Fragments arranged by size: 

                1428     572     482     360     248
 
BbvCI CC'TCA_GC

Cuts at:      0    1873    2005    3090 
   Size:       1873     132    1085

  Fragments arranged by size: 

                1873    1085     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1002    1036    3090 
   Size:       1002      34    2054

  Fragments arranged by size: 

                2054    1002      34
 
BclI T'GATC_A

Cuts at:      0    1367    3090 
   Size:       1367    1723
 
BglI GCCn_nnn'nGGC

Cuts at:      0     996    1313    3090 
   Size:        996     317    1777

  Fragments arranged by size: 

                1777     996     317
 
BglII A'GATC_T

Cuts at:      0    2846    3090 
   Size:       2846     244
 
BlpI GC'TnA_GC

Cuts at:      0      76    1568    2674    2741    3090 
   Size:         76    1492    1106      67     349

  Fragments arranged by size: 

                1492    1106     349      76      67
 
Bme1580I G_kGCm'C

Cuts at:      0     194     637     753    1291    1952    1990    2087    2983 
   Size:        194     443     116     538     661      38      97     896

Cuts at:   2983    3090 
   Size:        107

  Fragments arranged by size: 

                 896     661     538     443     194     116     107      97
                  38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1923    2698    3090 
   Size:       1923     775     392

  Fragments arranged by size: 

                1923     775     392
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    2050    3020    3028    3090 
   Size:        721    1329     970       8      62

  Fragments arranged by size: 

                1329     970     721      62       8
 
Bpu10I CC'TnA_GC

Cuts at:      0    1873    2005    2060    3090 
   Size:       1873     132      55    1030

  Fragments arranged by size: 

                1873    1030     132      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1075    3090 
   Size:        262     813    2015

  Fragments arranged by size: 

                2015     813     262
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     712     801     888    1668    2191    3004    3090 
   Size:        712      89      87     780     523     813      86

  Fragments arranged by size: 

                 813     780     712     523      89      87      86
 
BsaAI yAC'GTr

Cuts at:      0    2028    3090 
   Size:       2028    1062
 
BsaBI GATnn'nnATC

Cuts at:      0     520    3090 
   Size:        520    2570
 
BsaHI Gr'CG_yC

Cuts at:      0      25      35    2159    3090 
   Size:         25      10    2124     931

  Fragments arranged by size: 

                2124     931      25      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283     829     859     922     952    3001    3031 
   Size:        253      30     546      30      63      30    2049      30

Cuts at:   3031    3090 
   Size:         59

  Fragments arranged by size: 

                2049     546     253      63      59      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     406    1389    1738    1830    2485    2548    2601    2872 
   Size:        406     983     349      92     655      63      53     271

Cuts at:   2872    3090 
   Size:        218

  Fragments arranged by size: 

                 983     655     406     349     271     218      92      63
                  53
 
BseYI C'CCAG_C

Cuts at:      0     124     221     325     410    1235    1292    2385    2983 
   Size:        124      97     104      85     825      57    1093     598

Cuts at:   2983    3090 
   Size:        107

  Fragments arranged by size: 

                1093     825     598     124     107     104      97      85
                  57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     250    3090 
   Size:         66     184    2840

  Fragments arranged by size: 

                2840     184      66
 
BsiEI CG_ry'CG

Cuts at:      0     182    3090 
   Size:        182    2908
 
BsiHKAI G_wGCw'C

Cuts at:      0     206    1291    1574    1790    1943    2033    2066    2747 
   Size:        206    1085     283     216     153      90      33     681

Cuts at:   2747    3074    3090 
   Size:        327      16

  Fragments arranged by size: 

                1085     681     327     283     216     206     153      90
                  33      16
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      16    2924    3090 
   Size:         16    2908     166

  Fragments arranged by size: 

                2908     166      16
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     194     206     236     637     652     753    1291 
   Size:         92     102      12      30     401      15     101     538

Cuts at:   1291    1514    1574    1790    1840    1943    1952    1990    2033 
   Size:        223      60     216      50     103       9      38      43

Cuts at:   2033    2066    2087    2747    2983    3074    3090 
   Size:         33      21     660     236      91      16

  Fragments arranged by size: 

                 660     538     401     236     223     216     103     102
                 101      92      91      60      50      43      38      33
                  30      21      16      15      12       9
 
BspHI T'CATG_A

Cuts at:      0    2318    3090 
   Size:       2318     772
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     272     305     476     740    1535    2173    2883    3090 
   Size:        272      33     171     264     795     638     710     207

  Fragments arranged by size: 

                 795     710     638     272     264     207     171      33
            
 
BsrDI GCAATG_nn'

Cuts at:      0     785    2135    3090 
   Size:        785    1350     955

  Fragments arranged by size: 

                1350     955     785
 
BsrFI r'CCGG_y

Cuts at:      0     320    3090 
   Size:        320    2770
 
BsrGI T'GTAC_A

Cuts at:      0    2993    3090 
   Size:       2993      97
 
BssSI C'ACGA_G

Cuts at:      0     331    3090 
   Size:        331    2759
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152    2055    3090 
   Size:        152    1903    1035

  Fragments arranged by size: 

                1903    1035     152
 
BstEII G'GTnAC_C

Cuts at:      0     540    1084    3090 
   Size:        540     544    2006

  Fragments arranged by size: 

                2006     544     540
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     684     745     825    1344    1548    1782    2211    2460 
   Size:        684      61      80     519     204     234     429     249

Cuts at:   2460    3090 
   Size:        630

  Fragments arranged by size: 

                 684     630     519     429     249     234     204      80
                  61
 
BstYI r'GATC_y

Cuts at:      0    2165    2606    2846    3090 
   Size:       2165     441     240     244

  Fragments arranged by size: 

                2165     441     244     240
 
BtgI C'CryG_G

Cuts at:      0     683     791    1730    3090 
   Size:        683     108     939    1360

  Fragments arranged by size: 

                1360     939     683     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1527    1548    1755    2058    3090 
   Size:       1527      21     207     303    1032

  Fragments arranged by size: 

                1527    1032     303     207      21
 
BtsI GCAGTG_nn'

Cuts at:      0      70      87    3090 
   Size:         70      17    3003

  Fragments arranged by size: 

                3003      70      17
 
ClaI AT'CG_AT

Cuts at:      0     182    3090 
   Size:        182    2908
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     582     617    3090 
   Size:        582      35    2473

  Fragments arranged by size: 

                2473     582      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      44     705    1286    1757    2093    3090 
   Size:         44     661     581     471     336     997

  Fragments arranged by size: 

                 997     661     581     471     336      44
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     973    3090 
   Size:        973    2117
 
EaeI y'GGCC_r

Cuts at:      0     692     815    1049    1130    1160    2022    2252    2577 
   Size:        692     123     234      81      30     862     230     325

Cuts at:   2577    2625    3090 
   Size:         48     465

  Fragments arranged by size: 

                 862     692     465     325     234     230     123      81
                  48      30
 
EarI CTCTTCn'nnn_

Cuts at:      0    1795    3090 
   Size:       1795    1295
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     979    1951    3090 
   Size:        979     972    1139

  Fragments arranged by size: 

                1139     979     972
 
EcoICRI GAG'CTC

Cuts at:      0    1572    2064    3090 
   Size:       1572     492    1026

  Fragments arranged by size: 

                1572    1026     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721     936    1617    2050    3020    3028    3090 
   Size:        721     215     681     433     970       8      62

  Fragments arranged by size: 

                 970     721     681     433     215      62       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280     736    1450    1894    2173    2464    2890    3019 
   Size:        280     456     714     444     279     291     426     129

Cuts at:   3019    3090 
   Size:         71

  Fragments arranged by size: 

                 714     456     444     426     291     280     279     129
                  71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     729    1095    1121    1209    1948    1949    2145    2457 
   Size:        729     366      26      88     739       1     196     312

Cuts at:   2457    3024    3090 
   Size:        567      66

  Fragments arranged by size: 

                 739     729     567     366     312     196      88      66
                  26       1
 
FspI TGC'GCA

Cuts at:      0     160    3090 
   Size:        160    2930
 
FspAI rTGC'GCAy

Cuts at:      0     160    3090 
   Size:        160    2930
 
HaeII r_GCGC'y

Cuts at:      0      57    1711    2414    2444    3090 
   Size:         57    1654     703      30     646

  Fragments arranged by size: 

                1654     703     646      57      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     253     285     829     861     922     954 
   Size:         76      32     145      32     544      32      61      32

Cuts at:    954    2652    2684    3001    3023    3033    3055    3090 
   Size:       1698      32     317      22      10      22      35

  Fragments arranged by size: 

                1698     544     317     145      76      61      35      32
                  32      32      32      32      22      22      10
 
HincII GTy'rAC

Cuts at:      0    2817    3090 
   Size:       2817     273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1622    2477    2709    3090 
   Size:       1622     855     232     381

  Fragments arranged by size: 

                1622     855     381     232
 
MscI TGG'CCA

Cuts at:      0     694     817    1132    1162    2024    3090 
   Size:        694     123     315      30     862    1066

  Fragments arranged by size: 

                1066     862     694     315     123      30
 
MslI CAynn'nnrTG

Cuts at:      0     299     790    1342    1546    1729    1780    2212    2323 
   Size:        299     491     552     204     183      51     432     111

Cuts at:   2323    3090 
   Size:        767

  Fragments arranged by size: 

                 767     552     491     432     299     204     183     111
                  51
 
MspA1I CmG'CkG

Cuts at:      0      50     103    1304    1461    1637    2389    3090 
   Size:         50      53    1201     157     176     752     701

  Fragments arranged by size: 

                1201     752     701     176     157      53      50
 
NaeI GCC'GGC

Cuts at:      0     322    3090 
   Size:        322    2768
 
NdeI CA'TA_TG

Cuts at:      0     198    3090 
   Size:        198    2892
 
NgoMIV G'CCGG_C

Cuts at:      0     320    3090 
   Size:        320    2770
 
NspI r_CATG'y

Cuts at:      0     159     304     441    3090 
   Size:        159     145     137    2649

  Fragments arranged by size: 

                2649     159     145     137
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     643     984    1139    1935    3090 
   Size:        643     341     155     796    1155

  Fragments arranged by size: 

                1155     796     643     341     155
 
PfoI T'CCnGG_A

Cuts at:      0     622     737    3090 
   Size:        622     115    2353

  Fragments arranged by size: 

                2353     622     115
 
PmlI CAC'GTG

Cuts at:      0    2028    3090 
   Size:       2028    1062
 
PpuMI rG'GwC_Cy

Cuts at:      0     729    1095    1121    1209    2145    3024    3090 
   Size:        729     366      26      88     936     879      66

  Fragments arranged by size: 

                 936     879     729     366      88      66      26
 
PspOMI G'GGCC_C

Cuts at:      0     633    1948    3090 
   Size:        633    1315    1142

  Fragments arranged by size: 

                1315    1142     633
 
PstI C_TGCA'G

Cuts at:      0    1264    3090 
   Size:       1264    1826
 
PvuI CG_AT'CG

Cuts at:      0     182    3090 
   Size:        182    2908
 
PvuII CAG'CTG

Cuts at:      0     103    1304    1461    1637    3090 
   Size:        103    1201     157     176    1453

  Fragments arranged by size: 

                1453    1201     176     157     103
 
SacI G_AGCT'C

Cuts at:      0    1574    2066    3090 
   Size:       1574     492    1024

  Fragments arranged by size: 

                1574    1024     492
 
SanDI GG'GwC_CC

Cuts at:      0    1121    3090 
   Size:       1121    1969
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1795    3090 
   Size:       1795    1295
 
SexAI A'CCwGG_T

Cuts at:      0    1753    3090 
   Size:       1753    1337
 
SfcI C'TryA_G

Cuts at:      0    1103    1260    3090 
   Size:       1103     157    1830

  Fragments arranged by size: 

                1830    1103     157
 
SmlI C'TyrA_G

Cuts at:      0     277    1090    3090 
   Size:        277     813    2000

  Fragments arranged by size: 

                2000     813     277
 
SphI G_CATG'C

Cuts at:      0     159     304     441    3090 
   Size:        159     145     137    2649

  Fragments arranged by size: 

                2649     159     145     137
 
StuI AGG'CCT

Cuts at:      0     557     937    2119    2549    3090 
   Size:        557     380    1182     430     541

  Fragments arranged by size: 

                1182     557     541     430     380
 
StyI C'CwwG_G

Cuts at:      0     848    1115    2453    2574    3090 
   Size:        848     267    1338     121     516

  Fragments arranged by size: 

                1338     848     516     267     121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     235     522     676     768     789    1306    1658    3090 
   Size:        235     287     154      92      21     517     352    1432

  Fragments arranged by size: 

                1432     517     352     287     235     154      92      21
            
 
TatI w'GTAC_w

Cuts at:      0    2993    3090 
   Size:       2993      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2327    2359    3090 
   Size:       2327      32     731

  Fragments arranged by size: 

                2327     731      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3015    3090 
   Size:       3015      75
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     702     900    1059    1155    1829    2211    3090 
   Size:        702     198     159      96     674     382     879

  Fragments arranged by size: 

                 879     702     674     382     198     159      96
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV     NspI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI     PvuI    PvuII
     SacI    SanDI     SapI    SexAI     SfcI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BciVI    BfrBI    BmgBI     BmtI     BplI    BsaWI    BsiWI
     BsmI    BspEI    BsrBI   BssHII    BstBI  BstZ17I   Bsu36I     DraI
     EagI    EcoRI    EcoRV     FalI     FseI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NcoI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI    RsrII    SacII     SalI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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