(Circular) (Six-base) MAPSORT of: pF1KB7272.seq Check: 2397 from: 1 to: 4439 pF1KB7272 4439 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 461 461 Size: 4439 AccI GT'mk_AC Cuts at: 1477 1477 Size: 4439 Acc65I G'GTAC_C Cuts at: 1455 1455 Size: 4439 AclI AA'CG_TT Cuts at: 4279 4279 Size: 4439 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 397 766 899 2264 2696 3049 3711 397 Size: 369 133 1365 432 353 662 1125 Fragments arranged by size: 1365 1125 662 432 369 353 133 AflIII A'CryG_T Cuts at: 1791 3591 3872 1791 Size: 1800 281 2358 Fragments arranged by size: 2358 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 469 469 Size: 4439 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 721 753 721 Size: 32 4407 AlwNI CAG_nnn'CTG Cuts at: 240 320 673 1083 1744 3182 240 Size: 80 353 410 661 1438 1497 Fragments arranged by size: 1497 1438 661 410 353 80 ApaI G_GGCC'C Cuts at: 205 653 205 Size: 448 3991 ApaLI G'TGCA_C Cuts at: 1360 3277 1360 Size: 1917 2522 ApoI r'AATT_y Cuts at: 510 1073 1443 3792 4048 510 Size: 563 370 2349 256 901 Fragments arranged by size: 2349 901 563 370 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4439 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4439 AvaI C'yCGr_G Cuts at: 1367 1459 1367 Size: 92 4347 BamHI G'GATC_C Cuts at: 1464 1464 Size: 4439 BanI G'GyrC_C Cuts at: 173 1455 1869 2117 2152 173 Size: 1282 414 248 35 2460 Fragments arranged by size: 2460 1282 414 248 35 BanII G_rGCy'C Cuts at: 205 653 765 953 1453 2483 205 Size: 448 112 188 500 1030 2161 Fragments arranged by size: 2161 1030 500 448 188 112 BbeI G_GCGC'C Cuts at: 1873 2121 1873 Size: 248 4191 BbvCI CC'TCA_GC Cuts at: 1354 1354 Size: 4439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 612 646 90 Size: 34 488 34 3883 Fragments arranged by size: 3883 488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2337 3393 4439 2337 Size: 1056 1046 2337 Fragments arranged by size: 2337 1056 1046 BclI T'GATC_A Cuts at: 1959 1959 Size: 4439 BglI GCCn_nnn'nGGC Cuts at: 112 142 112 Size: 30 4409 BglII A'GATC_T Cuts at: 1954 3991 1954 Size: 2037 2402 BlpI GC'TnA_GC Cuts at: 241 1551 241 Size: 1310 3129 Bme1580I G_kGCm'C Cuts at: 205 653 1364 2064 2157 3281 205 Size: 448 711 700 93 1124 1363 Fragments arranged by size: 1363 1124 711 700 448 93 BmrI ACTGGGnnnn_n' Cuts at: 249 504 1651 1732 2067 4211 249 Size: 255 1147 81 335 2144 477 Fragments arranged by size: 2144 1147 477 335 255 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2806 2806 Size: 4439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1061 1203 1061 Size: 142 4297 Bpu10I CC'TnA_GC Cuts at: 674 1354 674 Size: 680 3759 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1615 2494 3208 3506 1615 Size: 879 714 298 2548 Fragments arranged by size: 2548 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1051 4143 1051 Size: 3092 1347 BsaAI yAC'GTr Cuts at: 1792 1792 Size: 4439 BsaBI GATnn'nnATC Cuts at: 6 1953 3990 6 Size: 1947 2037 455 Fragments arranged by size: 2037 1947 455 BsaHI Gr'CG_yC Cuts at: 1870 2118 2820 4316 1870 Size: 248 702 1496 1993 Fragments arranged by size: 1993 1496 702 248 BsaWI w'CCGG_w Cuts at: 1628 2149 2827 3238 3385 3659 1628 Size: 521 678 411 147 274 2408 Fragments arranged by size: 2408 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 721 751 721 Size: 30 4409 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1147 1264 1147 Size: 117 4322 BseYI C'CCAG_C Cuts at: 1326 1864 2622 3287 3695 1326 Size: 538 758 665 408 2070 Fragments arranged by size: 2070 758 665 538 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 693 693 Size: 4439 BsiEI CG_ry'CG Cuts at: 104 137 264 1981 2027 3257 104 Size: 33 127 1717 46 1230 1286 Fragments arranged by size: 1717 1286 1230 127 46 33 BsiHKAI G_wGCw'C Cuts at: 247 765 1364 1453 2231 2421 3281 247 Size: 518 599 89 778 190 860 1405 Fragments arranged by size: 1405 860 778 599 518 190 89 BsmBI CGTCTCn'nnnn_ Cuts at: 255 284 255 Size: 29 4410 Bsp1286I G_dGCh'C Cuts at: 205 247 653 765 953 1364 1453 2064 2157 Size: 42 406 112 188 411 89 611 93 Cuts at: 2157 2231 2421 2483 3281 205 Size: 74 190 62 798 1363 Fragments arranged by size: 1363 798 611 411 406 190 188 112 93 89 74 62 42 BspHI T'CATG_A Cuts at: 2871 2871 Size: 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 461 812 1489 1703 2005 2386 461 Size: 351 677 214 302 381 2514 Fragments arranged by size: 2514 677 381 351 302 214 BsrBI CCG'CTC Cuts at: 50 2731 2785 4269 4434 50 Size: 2681 54 1484 165 55 Fragments arranged by size: 2681 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2351 2351 Size: 4439 BsrFI r'CCGG_y Cuts at: 111 170 2437 2618 111 Size: 59 2267 181 1932 Fragments arranged by size: 2267 1932 181 59 BssSI C'ACGA_G Cuts at: 769 975 2710 3418 769 Size: 206 1735 708 1790 Fragments arranged by size: 1790 1735 708 206 BstAPI GCAn_nnn'nTGC Cuts at: 1816 1816 Size: 4439 BstBI TT'CG_AA Cuts at: 508 2800 508 Size: 2292 2147 BstXI CCAn_nnnn'nTGG Cuts at: 936 1039 936 Size: 103 4336 BstYI r'GATC_y Cuts at: 1 1464 1931 1954 2289 2535 2841 2853 2939 Size: 1463 467 23 335 246 306 12 86 Cuts at: 2939 3991 1 Size: 1052 449 Fragments arranged by size: 1463 1052 467 449 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1263 1263 Size: 4439 BtgI C'CryG_G Cuts at: 140 210 536 551 1161 1289 140 Size: 70 326 15 610 128 3290 Fragments arranged by size: 3290 610 326 128 70 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2389 2570 2733 2389 Size: 181 163 4095 Fragments arranged by size: 4095 181 163 BtsI GCAGTG_nn' Cuts at: 1183 1284 1682 1183 Size: 101 398 3940 Fragments arranged by size: 3940 398 101 DraI TTT'AAA Cuts at: 1438 1438 Size: 4439 DraIII CAC_nnn'GTG Cuts at: 402 1157 402 Size: 755 3684 DrdI GACnn_nn'nnGTC Cuts at: 2145 3489 2145 Size: 1344 3095 EaeI y'GGCC_r Cuts at: 134 570 2024 2198 2589 2616 3961 134 Size: 436 1454 174 391 27 1345 612 Fragments arranged by size: 1454 1345 612 436 391 174 27 EagI C'GGCC_G Cuts at: 134 2024 134 Size: 1890 2549 EarI CTCTTCn'nnn_ Cuts at: 1241 2462 2672 1241 Size: 1221 210 3008 Fragments arranged by size: 3008 1221 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2647 3389 3535 4249 2647 Size: 742 146 714 2837 Fragments arranged by size: 2837 742 714 146 EcoICRI GAG'CTC Cuts at: 763 1451 763 Size: 688 3751 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 397 766 899 1061 1203 2264 2696 3049 3711 Size: 369 133 162 142 1061 432 353 662 Cuts at: 3711 397 Size: 1125 Fragments arranged by size: 1125 1061 662 432 369 353 162 142 133 EcoO109I rG'GnC_Cy Cuts at: 202 366 650 1109 1578 202 Size: 164 284 459 469 3063 Fragments arranged by size: 3063 469 459 284 164 EcoRI G'AATT_C Cuts at: 1073 1443 1073 Size: 370 4069 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 4439 FspI TGC'GCA Cuts at: 2220 2220 Size: 4439 HaeII r_GCGC'y Cuts at: 1873 2121 3351 4122 1873 Size: 248 1230 771 2190 Fragments arranged by size: 2190 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 559 591 2281 2311 2313 2343 559 Size: 32 1690 30 2 30 2655 Fragments arranged by size: 2655 1690 32 30 30 2 HincII GTy'rAC Cuts at: 1478 1478 Size: 4439 HindIII A'AGCT_T Cuts at: 1000 1000 Size: 4439 KasI G'GCGC_C Cuts at: 1869 2117 1869 Size: 248 4191 KpnI G_GTAC'C Cuts at: 1459 1459 Size: 4439 MluI A'CGCG_T Cuts at: 3872 3872 Size: 4439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 480 830 863 3198 3382 480 Size: 350 33 2335 184 1537 Fragments arranged by size: 2335 1537 350 184 33 MscI TGG'CCA Cuts at: 572 2200 572 Size: 1628 2811 MslI CAynn'nnrTG Cuts at: 588 773 2428 2555 588 Size: 185 1655 127 2472 Fragments arranged by size: 2472 1655 185 127 MspA1I CmG'CkG Cuts at: 142 1550 1864 2224 3008 3253 142 Size: 1408 314 360 784 245 1328 Fragments arranged by size: 1408 1328 784 360 314 245 NaeI GCC'GGC Cuts at: 113 172 2620 113 Size: 59 2448 1932 Fragments arranged by size: 2448 1932 59 NarI GG'CG_CC Cuts at: 1870 2118 1870 Size: 248 4191 NcoI C'CATG_G Cuts at: 551 551 Size: 4439 NdeI CA'TA_TG Cuts at: 1429 1429 Size: 4439 NgoMIV G'CCGG_C Cuts at: 111 170 2618 111 Size: 59 2448 1932 Fragments arranged by size: 2448 1932 59 NotI GC'GGCC_GC Cuts at: 134 134 Size: 4439 NspI r_CATG'y Cuts at: 1492 1992 3595 1492 Size: 500 1603 2336 Fragments arranged by size: 2336 1603 500 PasI CC'CwG_GG Cuts at: 861 861 Size: 4439 PciI A'CATG_T Cuts at: 3591 3591 Size: 4439 PflMI CCAn_nnn'nTGG Cuts at: 351 351 Size: 4439 PfoI T'CCnGG_A Cuts at: 1219 1219 Size: 4439 PmeI GTTT'AAAC Cuts at: 1438 1438 Size: 4439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 721 753 2885 2917 721 Size: 32 2132 32 2243 Fragments arranged by size: 2243 2132 32 32 PpuMI rG'GwC_Cy Cuts at: 366 366 Size: 4439 PspOMI G'GGCC_C Cuts at: 201 649 201 Size: 448 3991 PstI C_TGCA'G Cuts at: 1486 2171 1486 Size: 685 3754 PvuI CG_AT'CG Cuts at: 104 104 Size: 4439 PvuII CAG'CTG Cuts at: 1864 2224 1864 Size: 360 4079 RsrII CG'GwC_CG Cuts at: 2634 2634 Size: 4439 SacI G_AGCT'C Cuts at: 765 1453 765 Size: 688 3751 SacII CC_GC'GG Cuts at: 143 143 Size: 4439 SalI G'TCGA_C Cuts at: 1476 1476 Size: 4439 SapI GCTCTTCn'nnn_ Cuts at: 2462 2672 2462 Size: 210 4229 SbfI CC_TGCA'GG Cuts at: 1486 1486 Size: 4439 ScaI AGT'ACT Cuts at: 274 3956 274 Size: 3682 757 SfcI C'TryA_G Cuts at: 33 1482 2167 3135 3326 33 Size: 1449 685 968 191 1146 Fragments arranged by size: 1449 1146 968 685 191 SfoI GGC'GCC Cuts at: 1871 2119 1871 Size: 248 4191 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4439 SmaI CCC'GGG Cuts at: 1461 1461 Size: 4439 SmlI C'TyrA_G Cuts at: 1594 2509 3223 3485 1594 Size: 915 714 262 2548 Fragments arranged by size: 2548 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4439 SphI G_CATG'C Cuts at: 1492 1992 1492 Size: 500 3939 StyI C'CwwG_G Cuts at: 362 551 816 1573 1927 362 Size: 189 265 757 354 2874 Fragments arranged by size: 2874 757 354 265 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2022 2155 2614 2823 2022 Size: 133 459 209 3638 Fragments arranged by size: 3638 459 209 133 TatI w'GTAC_w Cuts at: 272 3954 272 Size: 3682 757 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1215 1247 3956 3988 1215 Size: 32 2709 32 1666 Fragments arranged by size: 2709 1666 32 32 Tth111I GACn'n_nGTC Cuts at: 2236 2236 Size: 4439 XbaI T'CTAG_A Cuts at: 1470 1470 Size: 4439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 594 1934 594 Size: 1340 3099 XmaI C'CCGG_G Cuts at: 1459 1459 Size: 4439 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AgeI AhdI AleI AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NheI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI