Restriction map for pF1KB7267
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AhdI        ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        BamHI       BbrPI       
BciVI       BclI        BglII       BlpI        BpmI        
Bpu1102I    BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
HphI        Ksp632I     KspI        MamI        MfeI        
MflI        MluI        MroI        MslI        MspA1I      
MunI        NcoI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        213  GGTACC               G^GTACC
AfaI          214  GTAC                 GT^AC
AflII          67  CTTAAG               C^TTAAG
AflIII        588  ACACGT               A^C[GA][CT]GT
AgeI          216  ACCGGT               A^CCGGT
Alw44I        727  GTGCAC               G^TGCAC
AlwI          818  GGATC                GGATC(4/5)
AlwNI         790  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          813  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       911  AGCGCT               AGC^GCT
ApaI          414  GGGCCC               GGGCC^C
ApaLI         727  GTGCAC               G^TGCAC
Asp718I       213  GGTACC               G^GTACC
AspHI         727  GTGCAC               G[AT]GC[AT]^C
AviII         469  TGCGCA               TGC^GCA
BalI          806  TGGCCA               TGG^CCA
BbsI           36  GAAGAC               GAAGAC(2/6)
BbvCI         888  CCTCAGC              CCTCAGC(-5/-2)
BfrI           67  CTTAAG               C^TTAAG
BpuAI          36  GAAGAC               GAAGAC(2/6)
BsaAI         589  CACGTA               [CT]AC^GT[GA]
BsaI          309  GGTCTC               GGTCTC(1/5)
BsaWI         216  ACCGGT               [AT]^CCGG[AT]
BseRI         660  CTCCTC               GAGGAG(10/8)
BsiHKAI       727  GTGCAC               G[AT]GC[AT]^C
BsmAI         310  GTCTC                GTCTC(1/5)
BspMI         765  GCAGGT               ACCTGC(4/8)
BsrBI         715  GAGCGG               CCGCTC(-3/-3)
BstXI         402  CCAGGCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        813  CCTGAGG              CC^T[ACGT]AGG
DsaI           31  CCGTGG               C^C[GA][CT]GG
EagI          245  CGGCCG               C^GGCCG
EclXI         245  CGGCCG               C^GGCCG
Eco47III      911  AGCGCT               AGC^GCT
Eco52I        245  CGGCCG               C^GGCCG
Eco81I        813  CCTGAGG              CC^T[ACGT]AGG
FspI          469  TGCGCA               TGC^GCA
KpnI          213  GGTACC               GGTAC^C
MaeIII        827  GTAAC                ^GT[ACGT]AC
MboII          36  GAAGA                GAAGA(8/7)
MluNI         806  TGGCCA               TGG^CCA
MscI          806  TGGCCA               TGG^CCA
MseI           68  TTAA                 T^TAA
NdeI          782  CATATG               CA^TATG
PaeR7I         19  CTCGAG               C^TCGAG
PinAI         216  ACCGGT               A^CCGGT
PpuMI         187  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI          214  GTAC                 GT^AC
SbfI          927  CCTGCAGG             CCTGCA^GG
SfaNI         458  GCATC                GCATC(5/9)
SmaI          559  CCCGGG               CCC^GGG
Sse8387I      927  CCTGCAGG             CCTGCA^GG
TfiI          613  GATTC                G^A[AT]TC
Tru9I          68  TTAA                 T^TAA
XhoI           19  CTCGAG               C^TCGAG
XmaCI         559  CCCGGG               C^CCGGG
XmaI          559  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          240  GGCGCC               G[GA]^CG[CT]C
              300  GGCGCC              
AvrII         339  CCTAGG               C^CTAGG
              628  CCTAGG              
BbeI          240  GGCGCC               GGCGC^C
              300  GGCGCC              
BcgI          645  CGAAGCGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              936  CGAGGGACGTGC        
BfaI          340  CTAG                 C^TAG
              629  CTAG                
BglI           96  GCCGGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              745  GCCTTCGAGGC         
BlnI          339  CCTAGG               C^CTAGG
              628  CCTAGG              
BsaHI         240  GGCGCC               G[GA]^CG[CT]C
              300  GGCGCC              
BsgI          915  CTGCAC               GTGCAG(16/14)
              907  GTGCAG              
BsiEI         245  CGGCCG               CG[GA][CT]^CG
              637  CGACCG              
BssHII        105  GCGCGC               G^CGCGC
              481  GCGCGC              
DpnII         669  GATC                 ^GATC
              819  GATC                
DraII         187  AGGACCC              [GA]G^G[ACGT]CC[CT]
              413  GGGGCCC             
EaeI          245  CGGCCG               [CT]^GGCC[GA]
              806  TGGCCA              
EcoO109I      187  AGGACCC              [GA]G^G[ACGT]CC[CT]
              413  GGGGCCC             
EcoT14I       339  CCTAGG               C^C[AT][AT]GG
              628  CCTAGG              
Hin1I         240  GGCGCC               G[GA]^CG[CT]C
              300  GGCGCC              
KasI          240  GGCGCC               G^GCGCC
              300  GGCGCC              
MaeI          340  CTAG                 C^TAG
              629  CTAG                
MaeII         590  ACGT                 A^CGT
              942  ACGT                
MboI          669  GATC                 ^GATC
              819  GATC                
NaeI           96  GCCGGC               GCC^GGC
              243  GCCGGC              
NarI          240  GGCGCC               GG^CGCC
              300  GGCGCC              
NdeII         669  GATC                 ^GATC
              819  GATC                
NgoMIV         96  GCCGGC               G^CCGGC
              243  GCCGGC              
PleI          359  GACTC                GAGTC(4/5)
              526  GAGTC               
Sau3AI        669  GATC                 ^GATC
              819  GATC                
SfoI          240  GGCGCC               GGC^GCC
              300  GGCGCC              
StyI          339  CCTAGG               C^C[AT][AT]GG
              628  CCTAGG              
TaqI           20  TCGA                 T^CGA
              749  TCGA                
Tsp509I       582  AATT                 ^AATT
              594  AATT                
TthHB8I        20  TCGA                 T^CGA
              749  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NlaIV       PstI        Sau96I      ScrFI       
SfcI        SmlI        TseI        TspRI       
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