Restriction map for pF1KB7267
 (Linear) (Six-base) MAPSORT of: KIBB7267.seq  Check: 7689  from: 1  to: 963



KIBB7267.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     756     963 
   Size:        756     207
 
Acc65I G'GTAC_C

Cuts at:      0     213     963 
   Size:        213     750
 
AfeI AGC'GCT

Cuts at:      0     913     963 
   Size:        913      50
 
AflII C'TTAA_G

Cuts at:      0      67     963 
   Size:         67     896
 
AflIII A'CryG_T

Cuts at:      0     588     963 
   Size:        588     375
 
AgeI A'CCGG_T

Cuts at:      0     216     963 
   Size:        216     747
 
AlwNI CAG_nnn'CTG

Cuts at:      0     795     963 
   Size:        795     168
 
ApaI G_GGCC'C

Cuts at:      0     418     963 
   Size:        418     545
 
ApaLI G'TGCA_C

Cuts at:      0     727     963 
   Size:        727     236
 
AvaI C'yCGr_G

Cuts at:      0      19     148     296     304     559     963 
   Size:         19     129     148       8     255     404

  Fragments arranged by size: 

                 404     255     148     129      19       8
 
AvrII C'CTAG_G

Cuts at:      0     339     628     963 
   Size:        339     289     335

  Fragments arranged by size: 

                 339     335     289
 
BanI G'GyrC_C

Cuts at:      0     213     219     225     240     300     405     768     963 
   Size:        213       6       6      15      60     105     363     195

  Fragments arranged by size: 

                 363     213     195     105      60      15       6       6
            
 
BanII G_rGCy'C

Cuts at:      0     155     295     418     963 
   Size:        155     140     123     545

  Fragments arranged by size: 

                 545     155     140     123
 
BbeI G_GCGC'C

Cuts at:      0     244     304     963 
   Size:        244      60     659

  Fragments arranged by size: 

                 659     244      60
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      43     963 
   Size:         43     920
 
BbvCI CC'TCA_GC

Cuts at:      0     889     963 
   Size:        889      74
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     634     668     925     959     963 
   Size:        634      34     257      34       4

  Fragments arranged by size: 

                 634     257      34      34       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0     102     751     963 
   Size:        102     649     212

  Fragments arranged by size: 

                 649     212     102
 
Bme1580I G_kGCm'C

Cuts at:      0     268     418     731     963 
   Size:        268     150     313     232

  Fragments arranged by size: 

                 313     268     232     150
 
BmgBI CAC'GTC

Cuts at:      0     943     963 
   Size:        943      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     229     963 
   Size:        229     734
 
Bpu10I CC'TnA_GC

Cuts at:      0     757     889     963 
   Size:        757     132      74

  Fragments arranged by size: 

                 757     132      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     963 
   Size:         61     902
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     315     963 
   Size:        315     648
 
BsaAI yAC'GTr

Cuts at:      0     591     963 
   Size:        591     372
 
BsaHI Gr'CG_yC

Cuts at:      0     241     301     963 
   Size:        241      60     662

  Fragments arranged by size: 

                 662     241      60
 
BsaWI w'CCGG_w

Cuts at:      0     216     963 
   Size:        216     747
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     651     963 
   Size:        651     312
 
BseYI C'CCAG_C

Cuts at:      0     504     963 
   Size:        504     459
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900     928     963 
   Size:        900      28      35

  Fragments arranged by size: 

                 900      35      28
 
BsiEI CG_ry'CG

Cuts at:      0     248     640     963 
   Size:        248     392     323

  Fragments arranged by size: 

                 392     323     248
 
BsiHKAI G_wGCw'C

Cuts at:      0     731     963 
   Size:        731     232
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     268     295     418     731     963 
   Size:        155     113      27     123     313     232

  Fragments arranged by size: 

                 313     232     155     123     113      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     756     963 
   Size:        756     207
 
BsrBI CCG'CTC

Cuts at:      0     717     963 
   Size:        717     246
 
BsrFI r'CCGG_y

Cuts at:      0      96     204     216     222     243     963 
   Size:         96     108      12       6      21     720

  Fragments arranged by size: 

                 720     108      96      21      12       6
 
BssHII G'CGCG_C

Cuts at:      0     105     481     963 
   Size:        105     376     482

  Fragments arranged by size: 

                 482     376     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     409     963 
   Size:        409     554
 
Bsu36I CC'TnA_GG

Cuts at:      0     814     963 
   Size:        814     149
 
BtgI C'CryG_G

Cuts at:      0      31     963 
   Size:         31     932
 
BtsI GCAGTG_nn'

Cuts at:      0     353     963 
   Size:        353     610
 
EaeI y'GGCC_r

Cuts at:      0     245     806     963 
   Size:        245     561     157

  Fragments arranged by size: 

                 561     245     157
 
EagI C'GGCC_G

Cuts at:      0     245     963 
   Size:        245     718
 
EcoO109I rG'GnC_Cy

Cuts at:      0     188     414     963 
   Size:        188     226     549

  Fragments arranged by size: 

                 549     226     188
 
FspI TGC'GCA

Cuts at:      0     471     963 
   Size:        471     492
 
FspAI rTGC'GCAy

Cuts at:      0     471     963 
   Size:        471     492
 
HaeII r_GCGC'y

Cuts at:      0     148     244     304     520     757     915     963 
   Size:        148      96      60     216     237     158      48

  Fragments arranged by size: 

                 237     216     158     148      96      60      48
 
KasI G'GCGC_C

Cuts at:      0     240     300     963 
   Size:        240      60     663

  Fragments arranged by size: 

                 663     240      60
 
KpnI G_GTAC'C

Cuts at:      0     217     963 
   Size:        217     746
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     338     963 
   Size:        153     185     625

  Fragments arranged by size: 

                 625     185     153
 
MscI TGG'CCA

Cuts at:      0     808     963 
   Size:        808     155
 
NaeI GCC'GGC

Cuts at:      0      98     245     963 
   Size:         98     147     718

  Fragments arranged by size: 

                 718     147      98
 
NarI GG'CG_CC

Cuts at:      0     241     301     963 
   Size:        241      60     662

  Fragments arranged by size: 

                 662     241      60
 
NdeI CA'TA_TG

Cuts at:      0     783     963 
   Size:        783     180
 
NgoMIV G'CCGG_C

Cuts at:      0      96     243     963 
   Size:         96     147     720

  Fragments arranged by size: 

                 720     147      96
 
PfoI T'CCnGG_A

Cuts at:      0     434     963 
   Size:        434     529
 
PpuMI rG'GwC_Cy

Cuts at:      0     188     963 
   Size:        188     775
 
PspOMI G'GGCC_C

Cuts at:      0     414     963 
   Size:        414     549
 
PspXI vC'TCGA_Gb

Cuts at:      0      19     963 
   Size:         19     944
 
PstI C_TGCA'G

Cuts at:      0     203     683     932     963 
   Size:        203     480     249      31

  Fragments arranged by size: 

                 480     249     203      31
 
SbfI CC_TGCA'GG

Cuts at:      0     932     963 
   Size:        932      31
 
SfcI C'TryA_G

Cuts at:      0     199     679     867     928     963 
   Size:        199     480     188      61      35

  Fragments arranged by size: 

                 480     199     188      61      35
 
SfoI GGC'GCC

Cuts at:      0     242     302     963 
   Size:        242      60     661

  Fragments arranged by size: 

                 661     242      60
 
SmaI CCC'GGG

Cuts at:      0     561     963 
   Size:        561     402
 
SmlI C'TyrA_G

Cuts at:      0      19      67      76     963 
   Size:         19      48       9     887

  Fragments arranged by size: 

                 887      48      19       9
 
StyI C'CwwG_G

Cuts at:      0     339     628     963 
   Size:        339     289     335

  Fragments arranged by size: 

                 339     335     289
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      16      48     416     448     598     630     963 
   Size:         16      32     368      32     150      32     333

  Fragments arranged by size: 

                 368     333     150      32      32      32      16
 
XhoI C'TCGA_G

Cuts at:      0      19     963 
   Size:         19     944
 
XmaI C'CCGG_G

Cuts at:      0     559     963 
   Size:        559     404
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI    AflII   AflIII     AgeI    AlwNI     ApaI
    ApaLI     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BstXI   Bsu36I     BtgI
     BtsI     EaeI     EagI EcoO109I     FspI    FspAI    HaeII     KasI
     KpnI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     PfoI
    PpuMI   PspOMI    PspXI     PstI     SbfI     SfcI     SfoI     SmaI
     SmlI     StyI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AhdI     AleI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI     BaeI    BamHI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI     BplI     BpmI    BsaBI    BsaXI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI    Hin4I   HincII  HindIII     HpaI
     MfeI     MluI     MslI   MspA1I     NcoI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI     ZraI
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