Restriction map for pF1KB7259
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        AlwI        AlwNI       AscI        
Asp718I     AviII       AvrII       BamHI       BbrPI       
BcgI        BciVI       BclI        BglI        BlnI        
BlpI        Bpu1102I    BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MaeIII      MfeI        MluI        MroI        
MspA1I      MunI        MvnI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tsp45I      Van91I      XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcyI          959  GGCGCC               G[GA]^CG[CT]C
AocI          754  CCTCAGG              CC^T[ACGT]AGG
ApaI          213  GGGCCC               GGGCC^C
AseI         1462  ATTAAT               AT^TAAT
AsnI         1462  ATTAAT               AT^TAAT
Asp700I       243  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1675  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          959  GGCGCC               GGCGC^C
BbvCI         183  CCTCAGC              CCTCAGC(-5/-2)
BglII        1320  AGATCT               A^GATCT
BsaAI        1569  TACGTG               [CT]AC^GT[GA]
BsaBI         859  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         959  GGCGCC               G[GA]^CG[CT]C
BsmFI         800  GGGAC                GGGAC(10/14)
BsmI         1003  GAATGC               GAATGC(1/-1)
BsrDI         821  CATTGC               GCAATG(2/0)
BsrFI          25  ACCGGC               [GA]^CCGG[CT]
BstAPI        306  GCAAGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1320  AGATCT               [GA]^GATC[CT]
Bsu36I        754  CCTCAGG              CC^T[ACGT]AGG
Cfr10I         25  ACCGGC               [GA]^CCGG[CT]
DraIII        980  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          532  CTCTTC               CTCTTC(1/4)
Eco81I        754  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      1391  ATGCAT               ATGCA^T
HgaI           13  GACGC                GACGC(5/10)
Hin1I         959  GGCGCC               G[GA]^CG[CT]C
HincII        667  GTTGAC               GT[CT]^[GA]AC
HindII        667  GTTGAC               GT[CT]^[GA]AC
KasI          959  GGCGCC               G^GCGCC
Ksp632I       532  CTCTTC               CTCTTC(1/4)
MaeII        1570  ACGT                 A^CGT
MamI          859  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1320  AGATCT               [GA]^GATC[CT]
NarI          959  GGCGCC               GG^CGCC
NsiI         1391  ATGCAT               ATGCA^T
PaeR7I        747  CTCGAG               C^TCGAG
PflFI        1675  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          520  GAGTC                GAGTC(4/5)
PpuMI        1405  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        1462  ATTAAT               AT^TAAT
SacI          728  GAGCTC               GAGCT^C
ScaI          137  AGTACT               AGT^ACT
SexAI         287  ACCTGGT              A^CC[AT]GGT
SfoI          959  GGCGCC               GGC^GCC
StuI          751  AGGCCT               AGG^CCT
Tth111I      1675  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          357  CCACCTGGAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          747  CTCGAG               C^TCGAG
XhoII        1320  AGATCT               [GA]^GATC[CT]
XmnI          243  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          593  GTCTAC               GT^[AC][GT]AC
             1672  GTAGAC              
AflII         837  CTTAAG               C^TTAAG
             1086  CTTAAG              
AhdI          802  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              901  GACATTATGTC         
Alw44I         21  GTGCAC               G^TGCAC
              325  GTGCAC              
Aor51HI        65  AGCGCT               AGC^GCT
               83  AGCGCT              
ApaLI          21  GTGCAC               G^TGCAC
              325  GTGCAC              
AspEI         802  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              901  GACATTATGTC         
AvaI           49  CCCGAG               C^[CT]CG[GA]G
              747  CTCGAG              
BalI          293  TGGCCA               TGG^CCA
              354  TGGCCA              
BcnI          171  CCGGG                CC^[GC]GG
             1383  CCGGG               
BfaI          898  CTAG                 C^TAG
             1591  CTAG                
BfrI          837  CTTAAG               C^TTAAG
             1086  CTTAAG              
BsmAI         568  GAGAC                GTCTC(1/5)
             1097  GAGAC               
BsoBI          49  CCCGAG               C^[CT]CG[GA]G
              747  CTCGAG              
BspMI         315  GCAGGT               ACCTGC(4/8)
             1127  GCAGGT              
BsrBI         682  CCGCTC               CCGCTC(-3/-3)
              114  GAGCGG              
BstXI         692  CCAGTCATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              717  CCATGGCAGTGG        
DraII         213  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1405  AGGACCC             
DsaI          380  CCATGG               C^C[GA][CT]GG
              717  CCATGG              
Eam1105I      802  GACCACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              901  GACATTATGTC         
Eco47III       65  AGCGCT               AGC^GCT
               83  AGCGCT              
EcoO109I      213  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1405  AGGACCC             
MaeI          898  CTAG                 C^TAG
             1591  CTAG                
MluNI         293  TGGCCA               TGG^CCA
              354  TGGCCA              
MscI          293  TGGCCA               TGG^CCA
              354  TGGCCA              
NciI          171  CCGGG                CC^[GC]GG
             1383  CCGGG               
NcoI          380  CCATGG               C^CATGG
              717  CCATGG              
NspI          207  GCATGT               [GA]CATG^[CT]
             1389  ACATGC              
TfiI          376  GATTC                G^A[AT]TC
             1025  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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