Restriction map for pF1KB7243
 (Linear) (Six-base) MAPSORT of: KIBB7243.seq  Check: 488  from: 1  to: 1374



KIBB7243.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     716    1019    1374 
   Size:        716     303     355

  Fragments arranged by size: 

                 716     355     303
 
AflIII A'CryG_T

Cuts at:      0     158    1374 
   Size:        158    1216
 
AleI CACnn'nnGTG

Cuts at:      0     100     208     643    1374 
   Size:        100     108     435     731

  Fragments arranged by size: 

                 731     435     108     100
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403    1374 
   Size:        403     971
 
AlwNI CAG_nnn'CTG

Cuts at:      0     183     225     447     756    1374 
   Size:        183      42     222     309     618

  Fragments arranged by size: 

                 618     309     222     183      42
 
ApaI G_GGCC'C

Cuts at:      0     658     856    1374 
   Size:        658     198     518

  Fragments arranged by size: 

                 658     518     198
 
AvaI C'yCGr_G

Cuts at:      0      48    1168    1374 
   Size:         48    1120     206

  Fragments arranged by size: 

                1120     206      48
 
BamHI G'GATC_C

Cuts at:      0     575    1374 
   Size:        575     799
 
BanI G'GyrC_C

Cuts at:      0     475     505     604    1374 
   Size:        475      30      99     770

  Fragments arranged by size: 

                 770     475      99      30
 
BanII G_rGCy'C

Cuts at:      0     151     485     658     856    1374 
   Size:        151     334     173     198     518

  Fragments arranged by size: 

                 518     334     198     173     151
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      18    1374 
   Size:         18    1356
 
BbvCI CC'TCA_GC

Cuts at:      0     823    1374 
   Size:        823     551
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     750     784    1374 
   Size:        750      34     590

  Fragments arranged by size: 

                 750     590      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     299     496     698    1327    1374 
   Size:        299     197     202     629      47

  Fragments arranged by size: 

                 629     299     202     197      47
 
BlpI GC'TnA_GC

Cuts at:      0     391     757     895    1374 
   Size:        391     366     138     479

  Fragments arranged by size: 

                 479     391     366     138
 
Bme1580I G_kGCm'C

Cuts at:      0     609     658     856    1374 
   Size:        609      49     198     518

  Fragments arranged by size: 

                 609     518     198      49
 
BmgBI CAC'GTC

Cuts at:      0     161    1374 
   Size:        161    1213
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1222    1374 
   Size:       1222     152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     959     963    1374 
   Size:        587     372       4     411

  Fragments arranged by size: 

                 587     411     372       4
 
Bpu10I CC'TnA_GC

Cuts at:      0     823    1374 
   Size:        823     551
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      60     461    1147    1374 
   Size:         60     401     686     227

  Fragments arranged by size: 

                 686     401     227      60
 
BsaHI Gr'CG_yC

Cuts at:      0    1286    1374 
   Size:       1286      88
 
BsaWI w'CCGG_w

Cuts at:      0     585    1374 
   Size:        585     789
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      62      92     932     962    1374 
   Size:         62      30     840      30     412

  Fragments arranged by size: 

                 840     412      62      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     141     246     325     604     757    1165    1374 
   Size:         70      71     105      79     279     153     408     209

  Fragments arranged by size: 

                 408     279     209     153     105      79      71      70
            
 
BseYI C'CCAG_C

Cuts at:      0      39     338    1162    1374 
   Size:         39     299     824     212

  Fragments arranged by size: 

                 824     299     212      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260    1111    1374 
   Size:        260     851     263

  Fragments arranged by size: 

                 851     263     260
 
BsiEI CG_ry'CG

Cuts at:      0    1250    1374 
   Size:       1250     124
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     485     676    1374 
   Size:        107     378     191     698

  Fragments arranged by size: 

                 698     378     191     107
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     954    1374 
   Size:        954     420
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     151     485     609     658     676     856    1374 
   Size:        107      44     334     124      49      18     180     518

  Fragments arranged by size: 

                 518     334     180     124     107      49      44      18
            
 
BspEI T'CCGG_A

Cuts at:      0     585    1374 
   Size:        585     789
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      12    1374 
   Size:         12    1362
 
BsrFI r'CCGG_y

Cuts at:      0     452    1257    1374 
   Size:        452     805     117

  Fragments arranged by size: 

                 805     452     117
 
BsrGI T'GTAC_A

Cuts at:      0    1109    1374 
   Size:       1109     265
 
BssSI C'ACGA_G

Cuts at:      0     769    1129    1374 
   Size:        769     360     245

  Fragments arranged by size: 

                 769     360     245
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     363    1374 
   Size:        363    1011
 
BstYI r'GATC_y

Cuts at:      0     575    1374 
   Size:        575     799
 
Bsu36I CC'TnA_GG

Cuts at:      0    1101    1374 
   Size:       1101     273
 
BtgI C'CryG_G

Cuts at:      0     371     461    1374 
   Size:        371      90     913

  Fragments arranged by size: 

                 913     371      90
 
DraIII CAC_nnn'GTG

Cuts at:      0     874    1374 
   Size:        874     500
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     712    1374 
   Size:        712     662
 
EaeI y'GGCC_r

Cuts at:      0     488    1247    1295    1374 
   Size:        488     759      48      79

  Fragments arranged by size: 

                 759     488      79      48
 
EagI C'GGCC_G

Cuts at:      0    1247    1374 
   Size:       1247     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     172    1363    1374 
   Size:        172    1191      11

  Fragments arranged by size: 

                1191     172      11
 
EcoICRI GAG'CTC

Cuts at:      0     483    1374 
   Size:        483     891
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     959     963    1374 
   Size:        587     372       4     411

  Fragments arranged by size: 

                 587     411     372       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      88    1129    1374 
   Size:         88    1041     245

  Fragments arranged by size: 

                1041     245      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44     222     244     444     623     654     655    1374 
   Size:         44     178      22     200     179      31       1     719

  Fragments arranged by size: 

                 719     200     179     178      44      31      22       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     576    1374 
   Size:        576     798
 
HaeII r_GCGC'y

Cuts at:      0     319    1374 
   Size:        319    1055
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     560     567     592     599     932     964 
   Size:        133      32     395       7      25       7     333      32

Cuts at:    964    1374 
   Size:        410

  Fragments arranged by size: 

                 410     395     333     133      32      32      25       7
                   7
 
HincII GTy'rAC

Cuts at:      0     528    1374 
   Size:        528     846
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     728    1374 
   Size:        496     232     646

  Fragments arranged by size: 

                 646     496     232
 
MscI TGG'CCA

Cuts at:      0    1297    1374 
   Size:       1297      77
 
MslI CAynn'nnrTG

Cuts at:      0     100     208     643    1374 
   Size:        100     108     435     731

  Fragments arranged by size: 

                 731     435     108     100
 
MspA1I CmG'CkG

Cuts at:      0     827     858     894     983    1032    1079    1374 
   Size:        827      31      36      89      49      47     295

  Fragments arranged by size: 

                 827     295      89      49      47      36      31
 
NaeI GCC'GGC

Cuts at:      0    1259    1374 
   Size:       1259     115
 
NgoMIV G'CCGG_C

Cuts at:      0    1257    1374 
   Size:       1257     117
 
NspI r_CATG'y

Cuts at:      0    1224    1374 
   Size:       1224     150
 
PasI CC'CwG_GG

Cuts at:      0     849    1066    1374 
   Size:        849     217     308

  Fragments arranged by size: 

                 849     308     217
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     225     447    1374 
   Size:        225     222     927

  Fragments arranged by size: 

                 927     225     222
 
PfoI T'CCnGG_A

Cuts at:      0      10     959    1374 
   Size:         10     949     415

  Fragments arranged by size: 

                 949     415      10
 
PpuMI rG'GwC_Cy

Cuts at:      0     444    1374 
   Size:        444     930
 
PshAI GACnn'nnGTC

Cuts at:      0     547    1374 
   Size:        547     827
 
PspOMI G'GGCC_C

Cuts at:      0     654     852    1374 
   Size:        654     198     522

  Fragments arranged by size: 

                 654     522     198
 
PspXI vC'TCGA_Gb

Cuts at:      0    1168    1374 
   Size:       1168     206
 
PstI C_TGCA'G

Cuts at:      0     299    1374 
   Size:        299    1075
 
PvuII CAG'CTG

Cuts at:      0     983    1374 
   Size:        983     391
 
SacI G_AGCT'C

Cuts at:      0     485    1374 
   Size:        485     889
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1363    1374 
   Size:       1363      11
 
SbfI CC_TGCA'GG

Cuts at:      0     299    1374 
   Size:        299    1075
 
ScaI AGT'ACT

Cuts at:      0     556    1374 
   Size:        556     818
 
SfcI C'TryA_G

Cuts at:      0     295     708    1374 
   Size:        295     413     666

  Fragments arranged by size: 

                 666     413     295
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     496    1374 
   Size:        496     878
 
SmlI C'TyrA_G

Cuts at:      0    1168    1374 
   Size:       1168     206
 
StuI AGG'CCT

Cuts at:      0      62    1106    1374 
   Size:         62    1044     268

  Fragments arranged by size: 

                1044     268      62
 
StyI C'CwwG_G

Cuts at:      0    1346    1374 
   Size:       1346      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     369     454     592     646     691    1281    1374 
   Size:        369      85     138      54      45     590      93

  Fragments arranged by size: 

                 590     369     138      93      85      54      45
 
TatI w'GTAC_w

Cuts at:      0     554    1109    1374 
   Size:        554     555     265

  Fragments arranged by size: 

                 555     554     265
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94     344     376     860     892    1374 
   Size:         62      32     250      32     484      32     482

  Fragments arranged by size: 

                 484     482     250      62      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1015    1374 
   Size:       1015     359
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     846    1374 
   Size:        846     528
 
XhoI C'TCGA_G

Cuts at:      0    1168    1374 
   Size:       1168     206
 

 Enzymes that do cut:

     AccI   AflIII     AleI     AlfI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrFI    BsrGI    BssSI    BstXI    BstYI   Bsu36I     BtgI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
    PshAI   PspOMI    PspXI     PstI    PvuII     SacI     SapI     SbfI
     ScaI     SfcI     SfiI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BciVI     BclI    BfrBI    BglII     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsiWI     BsmI    BspHI    BsrBI    BsrDI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI     PsiI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XmaI     XmnI     ZraI
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